Navigation Links
Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance

w-abundance primer set must be individually determined.

How the RT-PCR Primers May Be Used

Figure 1

When analyzing cDNA quality, we used these control RT-PCR primers many different ways: For example, in Figure 1 and Figure 2, we used a number of the control primer sets to conduct a PCR analysis on lambda libraries derived from several human tissues. We wanted to detect an amplification product, as opposed to evaluate the relative amounts of different transcripts. Consequently, we used a large number of cycles (35 cycles) to maximize the signal. Under these conditions, the plateau effect obscured any quantitative relationship that might exist between the abundance of the transcript and accumulation of PCR product. The results in Figure 1 demonstrate that transcripts for b-actin, g-actin, and protein phosphatase 1 catalytic subunit are present in libraries generated from the lung, muscle, spleen, heart, and liver RNA. Other experiments confirmed their expression in the ovary, testis, cerebellum, and pancreas (data not shown).

Figure 2 shows PCR results obtained with primer pairs for the low-abundance genes ARF1 and ARF3 when tested on lambda libraries derived from the lung and spleen. In every instance, the size of the PCR product was consistent with cDNA, not gDNA, as the source of the PCR signal. These results (Figure 1 and Figure 2 and data not shown) are consistent with the broad tissue distribution of these transcripts and the ability of the control RT-PCR primers to detect their presence.

As an alternative
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
2. Human t-PA Control Primer Set
3. Rapid PCR* Using Active Tube Temperature Control On Thermos PCR Machines
4. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
5. Control Your siRNA Research
6. Designing Controls for siRNA Experiments
7. RNAi: The Controls You Need
8. RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control
9. Long-Term Instrument Control and Connectivity Solutions
10. Rapamycin Optimized in 100 Liter Fermentor Using BioCommand Control Strategys
11. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
Post Your Comments:
(Date:9/17/2014)... 17, 2014 Shimadzu Scientific ... Chromatograph (2D-LC), which offers a powerful separation ... The system provides a new level of ... is beneficial to scientists analyzing pharmaceutical impurities, ... products. , Comprehensive 2D-LC combines two independent ...
(Date:9/17/2014)... , Sept. 17, 2014 BioClinica®, Inc., ... eClinical Solutions, today announced three of its experts are ... for Clinical Data Management to be held September 28 ... . The Company also announced the innovative ... overall quality in clinical trials acquired earlier this year, ...
(Date:9/17/2014)... Hussey Copper , a leader in ... copper, announces Gilmour Academy Ice Arena,s conversion of more ... solid touch surface to help protect against bacteria.  MD-Cu ... that kills greater than 99.9% of MRSA, E-coli and ... , Innovative Gilmour Academy ...
(Date:9/17/2014)... RXi Pharmaceuticals Corporation (NASDAQ: ... developing and commercializing innovative therapies addressing major unmet ... has been granted a patent by the United ... delivery system for delivering its "original" RNAi compounds ... treatment of diseases with an inflammatory component. Potential ...
Breaking Biology Technology:Shimadzu’s New Nexera-e Two Dimensional Comprehensive Liquid Chromatograph Empowers Laboratories to Separate Even the Most Complex Mixtures 2BioClinica to Present at 20th Annual SCDM Conference 2BioClinica to Present at 20th Annual SCDM Conference 3Innovative Gilmour Academy Ice Arena changes more than 200 fixtures to Hussey Copper's MD-Cu29 antimicrobial copper to help protect against bacteria 2Innovative Gilmour Academy Ice Arena changes more than 200 fixtures to Hussey Copper's MD-Cu29 antimicrobial copper to help protect against bacteria 3RXi Pharmaceuticals Strengthens Intellectual Property Portfolio 2RXi Pharmaceuticals Strengthens Intellectual Property Portfolio 3RXi Pharmaceuticals Strengthens Intellectual Property Portfolio 4
... 25 Scimitar Equity, LLC issues a ... VOQP), entitled,"Creating Value; Turnaround Leverages Product Opportunities ... review is available at website: http://www.scimitarequity.com ... Jersey-based biotechnology company,dedicated to becoming a recognized ...
... 25 Mymetics,Corporation (OTC: MYMX), will be holding an ... PM New York Time to update all shareholders,on the ... Form 10-Q and to,address recent press articles., You ... at +1-866-591-4361 PIN 815,726 and from Europe at +41-800-329-326 ...
... 23 Eisai Corporation of,North America and its ... Food and Drug Administration (FDA) has approved a ... prevention of,chemotherapy-induced nausea and vomiting (CINV). ALOXI Capsules ... prevention of acute nausea and,vomiting following initial and ...
Cached Biology Technology:VioQuest Pharmaceuticals, Inc Q2/08 Review Issued by Scimitar Equity, LLC 2FDA Approves ALOXI(R) (Palonosetron HCl) Capsules for Prevention of Acute Chemotherapy-induced Nausea and Vomiting 2FDA Approves ALOXI(R) (Palonosetron HCl) Capsules for Prevention of Acute Chemotherapy-induced Nausea and Vomiting 3
(Date:9/16/2014)... The 36 Pit Fire began on September 13, 2014. ... It is currently over 2,300 acres in size and ... The 36 Pit Fire was calm Monday night due to ... establishing an anchor point and beginning to construct a fireline ... The following resources are working on the fire: , 7 ...
(Date:9/16/2014)... same viruses that make us sick can take up ... a sneeze, cough or other troublesome symptom, according to ... St. Louis. , On average, healthy individuals carry ... researchers report online in BioMed Central Biology . ... the diversity of viruses in healthy people. , ...
(Date:9/16/2014)... for the dinosaurs 66 million years ago decimated the ... extent than their deciduous peers, according to a study ... the journal PLOS Biology . , Applying ... fossilized leaves of angiosperms flowering plants excluding ... ecology of a diverse plant community thriving during a ...
Breaking Biology News(10 mins):Healthy humans make nice homes for viruses 2Healthy humans make nice homes for viruses 3Meteorite that doomed dinosaurs remade forests 2Meteorite that doomed dinosaurs remade forests 3Meteorite that doomed dinosaurs remade forests 4
... percent as compared to last year,s record size. According to measurements ... 27.7 million tonnes, compared to the 2006 record ozone loss of ... area and the depth of the ozone hole. The area of ... 220 Dobson Units is 24.7 million sq km, roughly the ...
... vaccine delivered through painless microneedles in patches applied ... to delivery through hypodermic needles, according to researchers ... Technology. Using new grants from the National Institutes ... five years, researchers from the two institutions plan ...
... help may be on the way for beleaguered growers ... Many varieties of the widely grown bottlegourd (Lagenaria siceraria) ... (ZYMV), a scourge of commercial cucurbits that includes pumpkins, ... ZYMV infects cucurbits throughout North America and in other ...
Cached Biology News:2007 ozone hole 'smaller than usual' 22007 ozone hole 'smaller than usual' 3Flu vaccine in painless skin patches under development at Emory, Georgia Tech with NIH grants 2Battling virus disease of watermelon with bottlegourds 2
HOXC11 Antibody...
HOXC11 Antibody...
PPOX Immunogen: PPOX (AAH02357, 378 a.a. ~ 478 a.a) partial recombinant protein with GST tag. Storage: -20 C, Avoid Freeze/Thaw Cycles *Available for distribution in the US only...
Rabbit anti MMP-13, Hinge Region MMP-13 Hinge Region...
Biology Products: