Navigation Links
Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance


Characterize gene distribution in your cDNA

Brenda Rogers Douglas McKenzie
Stratagene

We introduce six pairs of control RT-PCR primers that are designed to detect specific human gene transcripts with various expression levels. These primer sets provide insight into whether cDNA synthesis has been effective for all abundance classes of transcripts and whether full-length or near full-length cDNA species are present for those genes. The RT-PCR Primers for Genes of Varying Abundance Levels (human) consist of one primer pair each for a high- and medium-abundance gene transcript, and primer pairs for each of four low-abundance gene transcripts. The primer pairs are positioned toward the 5 end of the transcript to provide information about the effectiveness of first-strand synthesis. These RT-PCR primers span intron/exon junction(s) and are designed for use under high-stringency annealing conditions (60C). The primers are designed to have minimal self-complementarity and minimal capacity for self-priming or formation of primer-dimers. To complement the control RT-PCR primers, we also introduce probes for the same transcripts, so analysis of libraries can be conducted using standard probe technologies.

Generating cDNA from RNA is one of the principal methods used in gene discovery and gene expression analysis. While cDNA libraries have always played an important role in conventional screening technologies, they took on added importance in 1991 with the advent of the shotgun single pass, a partial sequencing of entire cDNA libraries.1 This new approach generated large collections of expressed sequence tags (ESTs) that did not correspond to known genes. The impact of this technology on new gene discovery has been enormous.

Similarly, the generation of normalized and sub
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
2. Human t-PA Control Primer Set
3. Rapid PCR* Using Active Tube Temperature Control On Thermos PCR Machines
4. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
5. Control Your siRNA Research
6. Designing Controls for siRNA Experiments
7. RNAi: The Controls You Need
8. RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control
9. Long-Term Instrument Control and Connectivity Solutions
10. Rapamycin Optimized in 100 Liter Fermentor Using BioCommand Control Strategys
11. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
Post Your Comments:
(Date:11/26/2014)... 2014 PMG Research, Inc. announced ... is a network of sites that provide clinical research services ... million patient lives through its partnerships with large physician practices ... hub site locations in the Southeastern United States and spans ... the board will be held by: Dr. Robert ...
(Date:11/26/2014)... CA (PRWEB) November 25, 2014 Silicon ... joins forces with RPM Alliance , provider of ... pharmaceutical companies. With the latest addition to its CRO ... free validated EDC system to India. , “We ... a robust and customizable EDC platform which is both ...
(Date:11/26/2014)... November 25, 2014 Global biostimulants ... 2019 growing at an estimated CAGR of 12.5%. ... healthy food products with least ecological impacts, farmers ... fertilizers to boost crop yield more effectively & ... that can improve the return on investments. , ...
(Date:11/26/2014)... and WILMINGTON, DE (PRWEB) November 26, 2014 ... Annual MPN Heroes recognition reception on December 5, 2014, ... Hematology’s annual meeting. The MPN Heroes event will honor ... in the field of myeloproliferative neoplasms (MPNs). , ... "Nashville," will discuss his family's cancer journey at the ...
Breaking Biology Technology:PMG Research Names Medical Advisory Board 2PMG Research Names Medical Advisory Board 3RPM Alliance Joins Clinovo’s CRO Partnership Program, Further Strengthening Its Presence in Southern California and India 22019 Biostimulants Market to Grow at a CAGR of 12.5% Says a New Research Report Available at RnRMarketResearch.com 22019 Biostimulants Market to Grow at a CAGR of 12.5% Says a New Research Report Available at RnRMarketResearch.com 32019 Biostimulants Market to Grow at a CAGR of 12.5% Says a New Research Report Available at RnRMarketResearch.com 4CURE Magazine and Incyte to Honor MPN Heroes 2CURE Magazine and Incyte to Honor MPN Heroes 3CURE Magazine and Incyte to Honor MPN Heroes 4
... ... for 87-Acre R&D/Manufacturing Facility , ... Philadelphia, Pa. (Vocus) September 8, 2009 -- BPG Properties, Ltd. ... 2750 Morris Road , a 705,000 square foot highly specialized research and development/manufacturing facility ...
... PriceSpective, a value strategy consultancy that provides ... pleased to announce that it has hired two senior experts ... President, and Sophie Murdoch, as a Director, will help further ... offerings. , , Colasante, who brings 20 ...
... , INCLINE VILLAGE, Nev., Sept. 8 ... announced revenue guidance for the third quarter ended September 30, ... for the third quarter of 2008. Royalty revenues are based ... $1.6 million for Synagis(R), which is marketed by MedImmune. When ...
Cached Biology Technology:BPG Properties, Ltd. Announces 2750 Morris Road in Lansdale, Pennsylvania is for Sale 2BPG Properties, Ltd. Announces 2750 Morris Road in Lansdale, Pennsylvania is for Sale 3PriceSpective Appoints Colasante as a Vice President and Murdoch as a Director 2PDL BioPharma Provides Third Quarter 2009 Revenue Guidance of Approximately $71 Million 2PDL BioPharma Provides Third Quarter 2009 Revenue Guidance of Approximately $71 Million 3
(Date:11/21/2014)... SAN JOSE, Calif. , Nov. 20, 2014 /PRNewswire/ ... a global leader in microcontroller (MCU) and touch technology ... digital temperature sensors with the widest V ... family delivers higher temperature accuracy and faster I 2 ... nonvolatile registers and serial EEPROM memory making them ideal ...
(Date:11/15/2014)... YORK , Nov. 13, 2014  While we may ... technology Doctor McCoy used in "Star Trek" to gain instant ... tools designed to work with smartphones and tablets for monitoring ... of the healthcare world. This may seem a tad Orwellian ... ready to adopt some of these technological opportunities into their ...
(Date:11/7/2014)... , Nov. 7, 2014  In conjunction with the ... Police Departments, The Community Foundation of the Verdugos has ... DNA JUSTICE PROJECT," a $250,000 initiative to keep the ... phase. In its first six months of existence, the Lab ... evidence support for local law enforcement. "Because our ...
Breaking Biology News(10 mins):Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 2Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 3Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 4Americans May Be Ready for a Brave New World of Healthcare 2Americans May Be Ready for a Brave New World of Healthcare 3Americans May Be Ready for a Brave New World of Healthcare 4Community Foundation Of The Verdugos Unveils Campaign To Fund Glendale-Burbank DNA Justice Project 2
... from the United Kingdom, the Energy Department,s National Renewable ... recently published a paper describing a novel cellulose-degrading enzyme ... commonly known as the gribble. Gribbles are ... to produce their own enzymes instead of using symbiotic ...
... expecting a very large "dead zone" in the Gulf ... in the Chesapeake Bay this year, based on several ... of Michigan, Louisiana State University, and the Louisiana ... of Mexico hypoxic "dead" zone will be between 7,286 ...
... Joshua Obar, Ph.D., Department of Immunology and Infectious ... 2013 ICAAC Young Investigator Award for his research on ... to infection. Obar earned his B.A. in ... on to complete his Ph.D. in Microbiology and Immunology ...
Cached Biology News:Novel enzyme from tiny gribble could prove a boon for biofuels research 2NOAA, partners predict possible record-setting deadzone for Gulf of Mexico 2NOAA, partners predict possible record-setting deadzone for Gulf of Mexico 3NOAA, partners predict possible record-setting deadzone for Gulf of Mexico 4The American Society for Microbiology honors Joshua Obar 2
Anti-MMP-14, catalytic domain, clone LEM-2/15.8 Immunogen: Synthetic peptide: amino acid sequence 218-233 within the catalytic domain Available Date: 38579...
Rabbit anti MMP-13, Hinge Region MMP-13 Hinge Region...
Evolutionarily conserved signaling intermediate in Toll pathway...
Phosphatidylinositol transfer protein beta isoform (PtdIns transfer protein beta) (PtdInsTP) (PI-TP-beta). [Source:Uniprot/SWISSPROT;Acc:P48739] Antigen: Recombinant Protein Epitope Signature Tag...
Biology Products: