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Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A

d (Shoup et al., 1997) or cleavage product. Similarly, a minor protein band in addition to the full size recombinant TGEV-M protein was detected by western blot (Figure 1C, lanes 3 and 5).

The results from this study suggest that CE-PAGE can be a useful semi-preparative tool to purify baculovirus-expressed TGEV viral proteins.


Acknowledgements

The authors thank Drs. H. Laude, D. J. Jackwood and D. I. Shoup for providing the recombinant baculovirus plasmid (N) or constructs (S, M).


References

1. Bohl, E. H., et al., Antibody responses in serum, colostrum and milk of swine after infection or vaccination with transmissible gastroenteritis virus, Infect. Immun., 6, 289301 (1972).

2. Lanza, I., et al., Lactogenic immunity and milk antibody isotypes to transmissible gastroenteritis virus in sows exposed to porcine respiratory coronavirus during pregnancy, Am. J. Vet. Res., 56, 739748 (1995).

3. Luckow, V. A. and Summers, M. D., Trends in the development of baculovirus expression vectors, Biotechnol., 6, 4755 (1988).

4. Saif, L. J. and Wesley, R. D., Transmissible gastroenteritis. In: Leman, A.D., et al., (Eds), Diseases of Swine. 7th edn., Iowa State University Press, Ames, pp. 362386 (1992).

5. Shoup, D. I., et al., Active and passive immune responses to transmissible gastroenteritis virus (TGEV) in swine inoculated with recombinant baculovirus-expressed TGEV spike glycoprotein vaccines, Am. J. Vet. Res., 58, 242250 (1997).

6. Stott, D. I., Immunoblotting and dot blotting, J. Immunol. Methods, 119
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