(Shoup et al., 1997) or cleavage product. Similarly, a minor
protein band in addition to the full size recombinant TGEV-M
protein was detected by western blot (Figure 1C, lanes 3 and 5).
The results from this study suggest that CE-PAGE can be a
useful semi-preparative tool to purify baculovirus-expressed
TGEV viral proteins.
The authors thank Drs. H. Laude, D. J. Jackwood and D. I. Shoup for providing
the recombinant baculovirus plasmid (N) or constructs (S, M).
Page: All 1 2 3 4 5 6 Related biology technology :1
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. A Versatile Power Supply for All Electrophoresis Applications2
. A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis3
. Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System4
. Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis5
. Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System6
. Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System7
. Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System8
. Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System9
. Oligonucleotide Purity Analysis by Capillary Electrophoresis10
. Preparative Nondenaturing Gel Electrophoresis Used in the Purification of an Esterase Involved in Insecticide Resistance, Rev C11
. Preparative Nondenaturing Gel Electrophoresis to Purify NADP-Specific Glutamate Dehydrogenase From Chlorella, Rev B