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Confirming RNAi Effects Is Now Easier Than Ever

d Library siRNA products. Each gene specific assay is designed to work under universal thermal cycling conditions and does not require optimization prior to use.


Figure 1. Knockdown of Survivin with a Silencer Validated siRNA and Verification with TaqMan Gene Expression Assays. HeLa cells were transfected with 30 nM of a Silencer Validated siRNA targeting survivin (BIRC5) or Silencer Negative Control #1 in quadruplicate. Forty-eight hours later, two sets of samples were stained with DAPI and analyzed by fluorescence microscopy. Total RNA was isolated from the other two sample sets using RNAqueous MAG-96 and then converted to cDNA. Target cDNA levels were analyzed in duplicate using a TaqMan Gene Expression Assay for survivin (Hs00153353_m1). Data shown here represent duplicate assays from single transfections. Survivin mRNA levels were reduced 80% in the survivin siRNA treated cells relative to control siRNA treated cells. Knockdown of survivin induced morphological changes to cell nuclei. The insets show representative cells from the survivin and control siRNA treated samples.


To make it easier than ever to find TaqMan Gene Expression Assays for your siRNA experiment, Ambion's online siRNA Database now provides links to individual gene expression assays on the Applied Biosystems' website. The siRNA Database provides information about Ambion's Silencer Pre-designed siRNAs targeting >98% of all human, mouse, and rat genes in NCBI's RefSeq database, as well as '"/>

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Related biology technology :

1. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
2. Effects of Thermal Cycler Well-To-Well Temperature Uniformity on PCR Results
3. Measuring Gene Silencing Effects by RT-PCR Without RNA Isolation

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