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Comparison of different protein,,,quantitation methods

This application note compares the accuracy and reproducibility of absolute protein quantitation of the Agilent 2100 bioanalyzer in combination with the Protein 200 Plus assay to the batch-based methods Lowry and Bradford as well as to denaturing gel-elctrophoresis (SDSPAGE). The results showed that the Agilent 2100 bioanalyzer provides fast and reliable absolute quantitation data comparable to the other methods investigated.
Determination of protein concentration is a routine procedure in many research laboratories. For example, it is required to calculate and monitor the protein yield after various enrichment or purification processes as well as to optimize and standardize downstream experiments such as protein-protein- interaction studies.
The Agilent 2100 bioanalyzer, developed in collaboration with Caliper Technologies Corp., provides a compact lab-on-a-chip system for the rapid and automated analysis of proteins, DNA, RNA and cells. Lab-on-a-chip technology integrates multiple experimental procedures, such as sample handling, separation staining/ destaining, detection and analysis in a single process. Together with the Protein 200 Plus LabChip kit sizing and analysis of proteins ranging in size from 14 to 200 kDa is possible. In addition, it has the ability to analyze relative quantitation, based on internal standards in each sample, and absolute quantitation based on user-defined calibration standards.
This application note compares the accuracy and reproducibility of absolute quantitation determined using the Protein 200 Plus assay for the following three methods: Lowry assay, Bradford assay and denaturing gel electrophoresis (SDS-PAGE) together with a documentation and analysis system.'"/>

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