Navigation Links
Comparison of Performance between the AlphaQuest - HTS and the Fusion α Multilabel Reader for Detection of cAMP

strument were observed as shown in Figure 4.

Discussion and Conclusions

The AlphaQuest-HTS is a high throughput-screening instrument designed to measure exclusively AlphaScreen reagents in miniaturized formats. Although it is capable of measuring AlphaScreen samples in 96-, 384-well format for assay development purposes, it is not capable of measuring other assay methods. For this reason, the Fusion system was developed to offer versatility and AlphaScreen capability in one system. The versatility of the Fusion system provides the ability to create and optimize assays based on methods currently being used such as prompt fluorescence, absorbance, FP and luminescence. In addition, the Fusion can now be equipped with the AlphaScreen option, which creates the opportunity for assay developers to delve into this unique chemistry, while continuing to use current assay methods.

The significant difference between the Fusion α and the AlphaQuest-HTS for the detection of AlphaScreen assays is in the throughput capabilities as shown in Table 2. When the first AlphaScreen assay is ready to screen, the AlphaQuest-HTS incorporates two lasers and four PMTs to measure four samples simultaneously for screening purposes, and can measure almost 1000 samples/hour.

Graphical representations of the percent bound curves, similar IC50 values, signal to noise, signal to background and ability to detect similar concentrations for an unknown sample confirm that the AlphaQuest-HTS and the Fusion α obtain the same results for a given assay. In addition, the regression analysis of raw data and percent bound values showed an extremely high correlation between the two instruments. This means that any assay that is developed on a Fusion α can be screened directly in the AlphaQuest-HTS without further development and optimization.

References


'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
2. Performance Comparisons of Commercial RT-PCR Systems
3. A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7 Cell Lines
4. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
5. TripleMaster PCR System: Direct Comparison with Competitors
6. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
7. Comparison of different protein quantitation methods
8. Comparison of Different Methods of mRNA Quantitation
9. Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A
10. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
11. Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A
Post Your Comments:
(Date:7/10/2014)... 2014 OMICS Group’s 5th International conference ... August, 2014 at Double Tree by Hilton Beijing, China ... in Analytical & Bioanalytical research methods only to facilitate ... this occasion, Dr. Srinubabu Gedela, MD of OMICS Group ... a remarkable one in bringing a unique and international ...
(Date:7/10/2014)...  Kainos Capital, a firm specializing in acquiring and ... has acquired the Slim-Fast brand from Unilever. Unilever will ... the transaction were not disclosed. Slim-Fast is ... markets ready-to-drink shakes, powders, bars and snacks to retail ... in the United Kingdom and ...
(Date:7/10/2014)... may help in the fight against terrorism with the ... of explosives with the use of light and special ... and Actuators B: Chemical , the researchers describe a ... concentrations as low as 6.3 ppm (parts per million). ... minutes. , "Traditionally explosives detection has involved looking for ...
(Date:7/10/2014)... our laptops and electric vehicles could store more energy ... help of a sponge-like silicon material. , Researchers ... used in one of the battery,s electrodes, as silicon ... of graphite. A paper describing the material,s performance as ... Nature Communications . , "Silicon has long been sought ...
Breaking Biology Technology:A Critical Review of Analytical Techniques for Improved Living Standards 2A Critical Review of Analytical Techniques for Improved Living Standards 3A Critical Review of Analytical Techniques for Improved Living Standards 4Kainos Capital Acquires Slim-Fast From Unilever 2Detecting trace amounts of explosives with light 2Silicon sponge improves lithium-ion battery performance 2Silicon sponge improves lithium-ion battery performance 3
... /Xinhua-PRNewswire-FirstCall/ -- China Medical,Technologies, Inc. (the "Company") ... device company that develops, manufactures and markets ... it will report selected,unaudited financial numbers including ... for the second fiscal quarter ended September ...
... Inc. ("Carna"),announced on October 27, 2008, the signing ... ("SUBC"). Under the terms of this,Agreement, SUBC will ... China., With China,s emergence as a strong ... companies have now established,research and production facilities in ...
... value and cost-effectiveness of spinal cord stimulation, ... BSX ) today welcomed an announcement by ... (NICE) recommending,the use of spinal cord stimulation (SCS) ... final guidance, NICE confirmed that,SCS is both safe ...
Cached Biology Technology:China Medical Technologies Announces Reporting Dates for the September Quarter Results and Addresses Investor Inquiries 2China Medical Technologies Announces Reporting Dates for the September Quarter Results and Addresses Investor Inquiries 3Carna Biosciences Signs Distribution Agreement with Shanghai Universal Biotech Company 2UK's NICE Recommends Spinal Cord Stimulation for Patients with Chronic Neuropathic Pain 2UK's NICE Recommends Spinal Cord Stimulation for Patients with Chronic Neuropathic Pain 3UK's NICE Recommends Spinal Cord Stimulation for Patients with Chronic Neuropathic Pain 4UK's NICE Recommends Spinal Cord Stimulation for Patients with Chronic Neuropathic Pain 5
(Date:7/11/2014)... Janeiro, Brazil- In the brains of all vertebrates, ... allows an electric or chemical signal to be ... synapses, which are the most abundant type of ... formation is crucial for learning, memory, perception and ... synapses critical for brain function. For instance, ...
(Date:7/10/2014)... Colo. , June 27, 2014  The ... the National Institute of Standards and Technology (NIST) ... of its academic research and forensic science expert ... The Board is an element of the NIST,s ... members are among the first appointments made to ...
(Date:7/10/2014)... 30, 2014  Aware, Inc. (NASDAQ: AWRE ... previously announced on June 26, 2014 that its Board ... $1.75 per share, or approximately $40 million in total.  ... 2014 and a payment date of July 24, 2014.  ... had set an ex-dividend date for this special cash ...
Breaking Biology News(10 mins):Blame it on the astrocytes 2Ten Members of the American Academy of Forensic Sciences Appointed to Forensic Science Standards Board (FSSB) 2Ten Members of the American Academy of Forensic Sciences Appointed to Forensic Science Standards Board (FSSB) 3NASDAQ Sets Ex-Dividend Date for Aware, Inc.'s Special Cash Dividend of $1.75 Per Share 2
... Physicists, chemists and engineers at the University of Pennsylvania ... of patchy particles, using charged, self-assembling molecules that may ... and perhaps be used in small batteries that store ... electrical charges of calcium ions just like the ...
... retinal prosthesis and fetal tissue transplant, restored some vision ... at Neuroscience 2009, the annual meeting of the Society ... news on brain science and health, may lead to ... an engineered protein restored vision in an animal model ...
... researchers from the United States, Korea, and France has sequenced ... E. coli bacteria, one used to study evolution ... practical applications. The findings will help guide future research and ... classical research that is the foundation of our understanding of ...
Cached Biology News:Penn team uses self-assembly to make molecule-sized particles with patches of charge 2Experimental treatments restore partial vision to blind people 2Genomes of 2 popular research strains of E. coli sequenced 2Genomes of 2 popular research strains of E. coli sequenced 3