Navigation Links
Comparison of Performance between the AlphaQuest - HTS and the Fusion α Multilabel Reader for Detection of cAMP

strument were observed as shown in Figure 4.

Discussion and Conclusions

The AlphaQuest-HTS is a high throughput-screening instrument designed to measure exclusively AlphaScreen reagents in miniaturized formats. Although it is capable of measuring AlphaScreen samples in 96-, 384-well format for assay development purposes, it is not capable of measuring other assay methods. For this reason, the Fusion system was developed to offer versatility and AlphaScreen capability in one system. The versatility of the Fusion system provides the ability to create and optimize assays based on methods currently being used such as prompt fluorescence, absorbance, FP and luminescence. In addition, the Fusion can now be equipped with the AlphaScreen option, which creates the opportunity for assay developers to delve into this unique chemistry, while continuing to use current assay methods.

The significant difference between the Fusion α and the AlphaQuest-HTS for the detection of AlphaScreen assays is in the throughput capabilities as shown in Table 2. When the first AlphaScreen assay is ready to screen, the AlphaQuest-HTS incorporates two lasers and four PMTs to measure four samples simultaneously for screening purposes, and can measure almost 1000 samples/hour.

Graphical representations of the percent bound curves, similar IC50 values, signal to noise, signal to background and ability to detect similar concentrations for an unknown sample confirm that the AlphaQuest-HTS and the Fusion α obtain the same results for a given assay. In addition, the regression analysis of raw data and percent bound values showed an extremely high correlation between the two instruments. This means that any assay that is developed on a Fusion α can be screened directly in the AlphaQuest-HTS without further development and optimization.

References


'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
2. Performance Comparisons of Commercial RT-PCR Systems
3. A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7 Cell Lines
4. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
5. TripleMaster PCR System: Direct Comparison with Competitors
6. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
7. Comparison of different protein quantitation methods
8. Comparison of Different Methods of mRNA Quantitation
9. Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A
10. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
11. Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A
Post Your Comments:
(Date:12/24/2014)... St. Louis, Missouri (PRWEB) December 23, 2014 ... and targeting Swiss biotechnology company, Syngenta, are in the ... for pretrial proceedings. The consolidated case is In Re: ... in the U.S. District Court for the District of ... litigation (MDL) has been handed over to U.S. District ...
(Date:12/24/2014)... (PRWEB) December 23, 2014 ... series is designed to introduce cutting edge ... users and partners of platform upgrades. This ... benefits; empowering users in their quest to ... tools. , According to SoundConnect’s Marketing Manager, ...
(Date:12/24/2014)... According to Ross Selinger, President of ... Global, the national office market continues to be strong, driven ... evidenced by the third quarter’s surprising 3.9% GDP, unemployment decreasing ... costs have held inflation down and the Fed is keeping ... market remaining soft. , This solid economic news is having ...
(Date:12/24/2014)... (PRWEB) December 23, 2014 Nashville Fertility ... was elected treasurer for the American Society for Reproductive ... knowledge and research in reproductive medicine. In his ... seat on the executive board and will have a ... Dr. Hill has actively supported ASRM since 1984 when ...
Breaking Biology Technology:Carey Danis & Lowe Reports on Syngenta GMO Corn Transfer Order 2SoundConnect Launches Leading Edge Business Communications Webinar Series 2SoundConnect Launches Leading Edge Business Communications Webinar Series 3ITRA Global Reports Long Island Is Out of Sync With The National Office Market 2ITRA Global Reports Long Island Is Out of Sync With The National Office Market 3Nashville Fertility Center Announces Dr. George A. Hill Selected to Serve as ASRM Treasurer for 2014-15 2Nashville Fertility Center Announces Dr. George A. Hill Selected to Serve as ASRM Treasurer for 2014-15 3
... -- Wisconsin residents would lose their ability to ... in the state under legislation proposed by state Sen. ... he would introduce legislation similar to that he proposed ... original proposal. He expects broad legislative support for the ...
... Oshkosh Truck Corp . and partners Rockwell Collins ... truck, TerraMax, qualified late Wednesday to compete in the $2 ... across the Mojave Desert on Saturday, Oct. 8. , , ... 2.9-mile qualifying obstacle courses at the California Speedway in Fontana, ...
... -- For some doctors, good bedside manner is ... skill -- one that a Madison medical educator hopes to ... envisions. , ,The development of the game is the next ... professor at the UW-Madison Department of Family Medicine ...
Cached Biology Technology:Bill would ban computers from landfills 2Bill would ban computers from landfills 3Oshkosh's robotic truck qualifies for DARPA challenge 2Video game technology could improve physician-patient interactions 2Video game technology could improve physician-patient interactions 3Video game technology could improve physician-patient interactions 4
(Date:12/5/2014)... 4, 2014  Tute Genomics, a leader in cloud-based ... Series A1 funding led by UK-based Eurovestech. Peak Ventures ... the investment round. "We are at a ... next-generation sequencing and seeks new approaches for the diagnosis, ... , MD MBA, and CEO of Tute Genomics. "One ...
(Date:12/3/2014)... 2, 2014   Marvin Test Solutions , ... solutions for military, aerospace, and manufacturing organizations, today ... successful TS-900 PXI semiconductor test platform . ... high-end systems to customers at a fraction of ... traditional ATE. "Our semiconductor customers ...
(Date:11/21/2014)... -- According to a new market research ... Access Control, Intrusion Detection, Parking Management, Under Vehicle Inspection), ... Geography - Global Forecasts to 2020", published by MarketsandMarkets, ... around $25 Billion in 2014 and is expected to ... of 8.69%. Browse 116 market data Tables ...
Breaking Biology News(10 mins):Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 2Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 3Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 2Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 3Industrial Security Systems Market Worth $38 Billion by 2020 2Industrial Security Systems Market Worth $38 Billion by 2020 3
... Thirty million years ago, before Ethiopia,s mountainous highlands ... zone had warmer soil temperatures, higher rainfall and different ... new research of fossil soils found in the central ... Earth Sciences at Southern Methodist University in Dallas and ...
... software* created at the National Institute of Standards and Technology ... to improve the efficiency of a pneumonia vaccine testing method ... Pneumonia is the world,s leading cause of death in children ... to elderly adults. The leading cause of pneumonia worldwide is ...
... on engineered proteins represent a new frontier for pharmaceutical ... form the basis of the next wonder drug, however, ... produce large quantities of the protein in a highly ... biochemist at the National Institute of Standards and Technology ...
Cached Biology News:Ethiopia's climate 27 million years ago had higher rainfall, warmer soil 2Scientists create NICE solution to pneumonia vaccine testing problems 2Biochemical 'on-switch' could solve protein purification challenge 2
Rat Antibody to Mouse TCR VB8.1,8.2:FITC Recognises the V beta 8.1 and V beta 8.2 determinants of the murine T cell receptor. Specificity: mouse TCR VB8.1 and 8.2...
... Alpha 1 Acid Glycoprotein ... Goats were immunized with highly ... Freund's adjuvant. The antiserum ... ensure specificity. For Research ...
...
... DuoSet ELISA Development kit contains the basic ... ELISAs to measure natural and recombinant canine ... culture supernates and serum.1 Each kit contains ... fifteen 96-well plates, provided that the following ...
Biology Products: