Navigation Links
Comparison of Performance between the AlphaQuest - HTS and the Fusion α Multilabel Reader for Detection of cAMP

strument were observed as shown in Figure 4.

Discussion and Conclusions

The AlphaQuest-HTS is a high throughput-screening instrument designed to measure exclusively AlphaScreen reagents in miniaturized formats. Although it is capable of measuring AlphaScreen samples in 96-, 384-well format for assay development purposes, it is not capable of measuring other assay methods. For this reason, the Fusion system was developed to offer versatility and AlphaScreen capability in one system. The versatility of the Fusion system provides the ability to create and optimize assays based on methods currently being used such as prompt fluorescence, absorbance, FP and luminescence. In addition, the Fusion can now be equipped with the AlphaScreen option, which creates the opportunity for assay developers to delve into this unique chemistry, while continuing to use current assay methods.

The significant difference between the Fusion α and the AlphaQuest-HTS for the detection of AlphaScreen assays is in the throughput capabilities as shown in Table 2. When the first AlphaScreen assay is ready to screen, the AlphaQuest-HTS incorporates two lasers and four PMTs to measure four samples simultaneously for screening purposes, and can measure almost 1000 samples/hour.

Graphical representations of the percent bound curves, similar IC50 values, signal to noise, signal to background and ability to detect similar concentrations for an unknown sample confirm that the AlphaQuest-HTS and the Fusion α obtain the same results for a given assay. In addition, the regression analysis of raw data and percent bound values showed an extremely high correlation between the two instruments. This means that any assay that is developed on a Fusion α can be screened directly in the AlphaQuest-HTS without further development and optimization.

References


'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
2. Performance Comparisons of Commercial RT-PCR Systems
3. A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7 Cell Lines
4. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
5. TripleMaster PCR System: Direct Comparison with Competitors
6. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
7. Comparison of different protein quantitation methods
8. Comparison of Different Methods of mRNA Quantitation
9. Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A
10. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
11. Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A
Post Your Comments:
(Date:12/2/2008)...DIEGO, Dec. 2 The Patient,Advocat...d. (ASX: IPD), is,pleased to announce the launch ...s MedCare Division, is offering the national, toll...e information and assistance to breast cancer,pat... side effects such as,lymphedema (swelling due to...
(Date:12/2/2008)...ewswire-Asia-FirstCall/ -- Tianyin,Pharmaceutical...and supplier,of modernized traditional Chinese me...ounced the implementation of a new sales and marke... hospital clients into three classes in order,to ... The new strategy has classified distributors ...
(Date:12/2/2008)...RNewswire/ -- Starch Medical Inc, a San Jose,CA-...E approval of its,PerClot(TM) Polysaccharide Hemo...le Polysaccharide Hemostat. Introduction of PerCl...an Union and other select international,markets. ...t will launch,during the 2nd quarter, 2009. , ...
(Date:12/2/2008)... Regado Biosciences, Inc., announc...newly created position of Senior Vice President of...ffective immediately. Ms. McDonald brings to Rega...eutical industry, with expertise heading business ...marketing. She will report to David J. Mazzo, Ph....
Breaking Biology Technology:New Program Offers Breast Cancer Clinicians and Patients Support for Pre-Operative Clinical Assessment and Ongoing Surveillance of Lymphedema 2New Program Offers Breast Cancer Clinicians and Patients Support for Pre-Operative Clinical Assessment and Ongoing Surveillance of Lymphedema 3Tianyin Pharmaceutical Co., Inc. Implements New Sales and Marketing Strategy to Drive Revenue Growth 2Tianyin Pharmaceutical Co., Inc. Implements New Sales and Marketing Strategy to Drive Revenue Growth 3Starch Medical Launches New, Bioinert Surgical Hemostats 2Ellen McDonald Joins Regado Biosciences as Senior Vice President of Business Operations and Chief Business Officer 2
...cessory among their standard travel gear starting ...w passports, mandated by State Department regulati...ate of the information featured on the passport,s ...engthen national security. , ,"The electronic pass...e currently have," said Bureau of Consular Affairs...
...toed a bill that would have banned cloning of huma...legislative director of Wisconsins Right to Life m...a science fiction movie. , ,We,re not talking abou...these embryos . . . not for stem cells but for org...s quoted as saying. , ,I must have missed somethin...
... announced a new eHealth Care Quality and Patient ...ion of electronic medical records in Wisconsin. , ...er of information technology at the most important...nt," Doyle said in a statement, citing statistics ...sary or redundant. "This board will develop a road...
... Council has teamed with JobSightUSA , a top-rank...test, to match job seekers with positions based on...JobSightUSA helps companies that are considering l...and hire professionals, while also assisting forme...he state. The site enables professional jobseekers...
Other Biology Technology:Passports to contain RFID chips next year 2Separating science fiction from reality in the human cloning debate 2Separating science fiction from reality in the human cloning debate 3Wisconsin pushes for adoption of electronic health records 2
(Date:12/2/2008)... 2, 2008 Scientists and engineers from two of the... will come together Dec. 8, along with academician...gies in moving oil and pumping blood. , Much l...pump blood through the body. Both systems need cl...ee of blockages or corrosion, to function with inc...
(Date:12/2/2008)..., N.J. Rutgers, The State University of New Jerse...m the Robert Wood Johnson Foundation to establish ...ts George H. Cook Campus. , Rutgers President Ric...lish the new institute during his Annual Address t...dress, McCormick made it clear the new institute w...
(Date:12/2/2008)... is available in German . , , Sometimes p... tricks. Scientists at the Max Planck Institute fo... the Colorado School of Mines have constructed mic...use to get ships into a bottle where the masts and... it is in the bottle. In the same way, the scienti...
(Date:12/2/2008)...Calif. Dec. 2, 2008 Presbycusis, or age-related ...ng loss. So, why do some people lose their hearin...r a pin drop? , The answer may be in a study r...etics . Advance access is available online at htt... "This is the first ever and largest genome-wide ...
Breaking Biology News(10 mins):Using the other guy's toolkit: Similarities of pumping blood, oil examined 2Rutgers receives US$10 million to establish Institute for Food, Nutrition and Health 2Ship-in-a-bottle kit on a microchip 2Ship-in-a-bottle kit on a microchip 3House Ear Institute, TGen and Belgian researchers identify gene in age-related hearing loss 2Hoviones Sales Grow 11 25 6280 1Hoviones Sales Grow 11 25 6280 2Photos 3A For the First Time Researchers Show Reversal of an Aging Marker in the Human Retina Correlated with Visual Improvement Using a Nutraceutical 22873 1Photos 3A For the First Time Researchers Show Reversal of an Aging Marker in the Human Retina Correlated with Visual Improvement Using a Nutraceutical 22873 2Photos 3A For the First Time Researchers Show Reversal of an Aging Marker in the Human Retina Correlated with Visual Improvement Using a Nutraceutical 22873 3New York Resident Elected President of Lions Clubs International 22871 1New York Resident Elected President of Lions Clubs International 22871 2H5N1 Virus Replikin Gene Counts Indicate a New More Virulent Influenza Cycle Has Begun 22869 1H5N1 Virus Replikin Gene Counts Indicate a New More Virulent Influenza Cycle Has Begun 22869 2
...y and vaccine design, researchers in the Biodesign...sfully turned tobacco plants into vaccine producti...e. The vaccine elicits a protective immune respons... a milestone in the future development of a new va...ped bacterium called Yersinia pestis, no longer in...
... which they believe could ultimately lead to the d...lly modified tobacco. Writing in Plant Biotechnolo...m St George,s, University of London along with res...e overcome a major barrier that has so far frustra...le "bioreactors" for vaccines. , By creating fusio...
...lk, the young of the African amphibian Boulengerul... eating the fat-rich outer layer of their mothers,...rchers that includes University of Michigan biolog...n today,s issue (April 13) of the journal Nature. ... that looks something like an earthworm---are born...
...ncreased over a larger area in the last century th...lished in Science reveals this week. , The study ... dramatically in the Northern Hemisphere in the la...g to the growing evidence of wide-scale global war...he Climatic Research Unit team at the University o...
Other Biology News:Plant-derived vaccines safeguard against deadly plague 2Plant-derived vaccines safeguard against deadly plague 3Do plants have the potential to vaccinate against HIV? 2African amphibians make extreme parental sacrifice: The skin off their backs 2African amphibians make extreme parental sacrifice: The skin off their backs 3
Rat Cardiac Fibroblasts (RCF) (>500,000 cells)
...rmulation of a rinsing buffer for use in autostain... as for manual immunostaining. It was developed in... of Pathology, University of Oklahoma, Oklahoma Ci...sing steps performed when doing immunocytochemical...
Phytohemagglutinin from Invitrogen
...t not structural information the AXIMA-LNR linear ...high throughput QA/QC applications and for high pe...lude oligonucleotide QA/QC, SNP analysis, protein ...d polymer analysis, with the ability to run up to ...
Biology Products: