Navigation Links
Comparison of Performance between the AlphaQuest - HTS and the Fusion α Multilabel Reader for Detection of cAMP

By Laura Gaidar, Kristin Prasauckas,
Kelly Carter-Allen and Loraine Upham
PerkinElmer Life Sciences, Inc.

Introduction

AlphaScreen technology is a highly sensitive, non-isotopic homogenous assay method developed for assay development and high throughput screening applications. PerkinElmer Life Sciences has developed two instruments for the detection of AlphaScreen reagents. The AlphaQuest-HTS system measures 96-, 384- and 1536-well plates with four detectors simultaneously to maximize throughput, and is dedicated to the detection of AlphaScreen reagents. The Fusion α Multilabel Reader is a multi-detection system, capable of measuring absorbance (Vis and UV), prompt fluorescence, fluorescence polarization, time-resolved fluorescence, luminescence and AlphaScreen reagents in 96-, 384- and, in most modes, 1536-well plates, with a single detector. The following study was conducted to compare the performance of each system for the detection of cAMP using the AlphaScreen cAMP detection kit.

Materials and Methods

The cAMP standard curves were prepared as described in the AlphaScreen cAMP detection kit (PerkinElmer #6760600M) technical data sheet instructions. Unlabeled cAMP (Sigma, St. Louis, MO #A9501) was used to compete against the biotinylated cAMP provided in the AlphaScreen cAMP kit. Total reagent volume was 25 μl in 384-well white OptiPlate (PerkinElmer #6005214). Competition of unlabeled cAMP at various concentrations against a fixed concentration of biotinylated cAMP was measured using two Fusion α systems and an AlphaQuest-HTS system. Figure 1 illustrates the AlphaScreen cAMP assay(1). One unknown cAMP sample was also run with each curve to test the accuracy of the interpolation. A regression analysis of the raw and transformed values was performed to determine how closely the ou
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
2. Performance Comparisons of Commercial RT-PCR Systems
3. A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7 Cell Lines
4. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
5. TripleMaster PCR System: Direct Comparison with Competitors
6. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
7. Comparison of different protein quantitation methods
8. Comparison of Different Methods of mRNA Quantitation
9. Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A
10. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
11. Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A
Post Your Comments:
(Date:8/28/2014)... --  SunTrust Robinson Humphrey (STRH) today announced ... equity research team in biotechnology and oilfield services.  ... research demonstrates our commitment to providing our valued ... investment decision making," said Biff Woodruff , ... make significant investments in differentiated content at a ...
(Date:8/28/2014)... 2014 A new market research ... is available from Strategic Consulting, Inc., the leading ... Microbiology Market Review, Fourth Edition: Global Review of ... tracks and compares microbiology test volumes, market values ... and Asia, and forecasts future volumes and market ...
(Date:8/27/2014)... 27, 2014 Varian Medical Systems today ... current Salt Lake City manufacturing facility and allow for ... to ultimately create 1,000 new full time jobs in ... “Utah is home to hardworking people with immense engineering ... with Varian’s success in the manufacturing of our state-of-the-art ...
(Date:8/27/2014)... Aug. 27, 2014   MSC , a healthcare ... today announced the appointment of Mary Beth Loesch ... 28 years of experience preparing companies for rapid growth ... Executive Vice President of Corporate Development and Healthcare. In ... business , as well as corporate strategy and marketing. ...
Breaking Biology Technology:SunTrust Robinson Humphrey Expands Equity Research in Healthcare and Energy 2SunTrust Robinson Humphrey Expands Equity Research in Healthcare and Energy 32 Billion Industrial Microbiology Tests Conducted for Product Quality and Safety Worldwide 22 Billion Industrial Microbiology Tests Conducted for Product Quality and Safety Worldwide 3Varian Medical Systems Groundbreaking on Facility Expansion Triggers Influx of New Jobs to Salt Lake 2Varian Medical Systems Groundbreaking on Facility Expansion Triggers Influx of New Jobs to Salt Lake 3MSC names Mary Beth Loesch President and CEO 2
... ROI Opportunity for Consumers in Shortest Period of ... examines the potential investment in targeted therapies that ... new report released today by the Deloitte Center ... adoption of personalized medicine to produce a positive ...
... Technology and consulting firm, Fadel Partners Inc., a certified ... over year revenue growth of 13% for 2008, and ... The company,s report stands in contrast to analyst estimates ... the U.S. IT services industry overall, as the economic ...
... Allow Development of Accurate Blood Test for Common Genetic ... Artemis Health Inc., a privately-held company dedicated to ... that it has acquired a co-exclusive worldwide license from ... prenatal diagnostic tests based on pioneering research from the ...
Cached Biology Technology:Adoption of Personalized Medicine May Yield Significant ROI; Time to Achieve Benefit Varies Among U.S. Health Care Stakeholders, According to Deloitte Report 2Adoption of Personalized Medicine May Yield Significant ROI; Time to Achieve Benefit Varies Among U.S. Health Care Stakeholders, According to Deloitte Report 3Adoption of Personalized Medicine May Yield Significant ROI; Time to Achieve Benefit Varies Among U.S. Health Care Stakeholders, According to Deloitte Report 4Fadel Partners Announces 13% Growth Despite IT Services Industry Downtrend 2Artemis Health Licenses Prenatal Cell-Free DNA Analysis Technology from Stanford University 2Artemis Health Licenses Prenatal Cell-Free DNA Analysis Technology from Stanford University 3
(Date:8/29/2014)... such as the prolific bloomer Emiliania huxleyi , ... discovered. The finding contradicts the common view that ... on scarce supplies of thiamine in the ocean to ... about the ocean," says CIFAR Senior Fellow Alexandra ... paper with CIFAR fellows John Archibald (Dalhousie ...
(Date:8/29/2014)... them, fold them: modern materials that are light, flexible ... artificial skin or electronic paper. , Making such concepts ... a new way of working with copper nanowires and ... , Previous success in the field of ultra-lightweight "aerogel ... gold and silver nanowires. , By turning instead ...
(Date:8/28/2014)... app called TX Invasives is now available from the ... Texas at Austin for identifying harmful non-native plant, insect ... in the United States cause about $137 billion in ... crops, clog waterways, kill native plants and more. , ... for reporting where invasive species occur, we,re more likely ...
Breaking Biology News(10 mins):Not all phytoplankton in the ocean need to take their vitamins 2Not all phytoplankton in the ocean need to take their vitamins 3Copper shines as flexible conductor 2Mobile app makes ID of harmful plants, insects in Texas a snap 2
... communities and the growth of sedimentary rock at Mammoth ... surprising discovery about the geological record of life and ... of sedimentary rock are dated, and how scientists might ... found microbes change the rate at which calcium carbonate ...
... Titled Assessment of Climate Change for the Baltic Sea Basin ... survey of past and possible future climate change in the ... in northern and mid-Europe. One of the key findings is ... by up to five degrees Celsius from now to 2100. ...
... oceans, lakes and even wet soil, diatoms are unicellular ... Curiously, these tiny phytoplankton could be harboring the next ... cell walls by laying down submicron-sized lines of silica, ... semiconductor industrysilicon. If we can genetically control that process, ...
Cached Biology News:Hot springs microbes hold key to dating sedimentary rocks, researchers say 2Climate change in the Baltic Sea basin -- past, present and future 2Climate change in the Baltic Sea basin -- past, present and future 3In diatom, scientists find genes that may level engineering hurdle 2
... Lambda Protein Phosphatase ( ... protein phosphatase with activity ... and tyrosine residues. It ... product of the ORF221 ...
... a high performance spectral scanning multimode ... With the advanced SkanIt Software it ... drug discovery assay development. Varioskan provides ... time-resolved fluorescence and photometric assays and ...
... refrigerated cold traps offers a choice of ... solvents or high volatility solvents. Ideal to ... evaporators. Also used for maintaining vapor-free gas ... in electron microscopes. Can be used as ...
96 Well Plate UB, RE bottom; consistent medium binding affinity for hydrophobic complexes and proteins....
Biology Products: