Navigation Links
Comparison of Performance between the AlphaQuest - HTS and the Fusion α Multilabel Reader for Detection of cAMP

By Laura Gaidar, Kristin Prasauckas,
Kelly Carter-Allen and Loraine Upham
PerkinElmer Life Sciences, Inc.

Introduction

AlphaScreen technology is a highly sensitive, non-isotopic homogenous assay method developed for assay development and high throughput screening applications. PerkinElmer Life Sciences has developed two instruments for the detection of AlphaScreen reagents. The AlphaQuest-HTS system measures 96-, 384- and 1536-well plates with four detectors simultaneously to maximize throughput, and is dedicated to the detection of AlphaScreen reagents. The Fusion α Multilabel Reader is a multi-detection system, capable of measuring absorbance (Vis and UV), prompt fluorescence, fluorescence polarization, time-resolved fluorescence, luminescence and AlphaScreen reagents in 96-, 384- and, in most modes, 1536-well plates, with a single detector. The following study was conducted to compare the performance of each system for the detection of cAMP using the AlphaScreen cAMP detection kit.

Materials and Methods

The cAMP standard curves were prepared as described in the AlphaScreen cAMP detection kit (PerkinElmer #6760600M) technical data sheet instructions. Unlabeled cAMP (Sigma, St. Louis, MO #A9501) was used to compete against the biotinylated cAMP provided in the AlphaScreen cAMP kit. Total reagent volume was 25 μl in 384-well white OptiPlate (PerkinElmer #6005214). Competition of unlabeled cAMP at various concentrations against a fixed concentration of biotinylated cAMP was measured using two Fusion α systems and an AlphaQuest-HTS system. Figure 1 illustrates the AlphaScreen cAMP assay(1). One unknown cAMP sample was also run with each curve to test the accuracy of the interpolation. A regression analysis of the raw and transformed values was performed to determine how closely the ou
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
2. Performance Comparisons of Commercial RT-PCR Systems
3. A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7 Cell Lines
4. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
5. TripleMaster PCR System: Direct Comparison with Competitors
6. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
7. Comparison of different protein quantitation methods
8. Comparison of Different Methods of mRNA Quantitation
9. Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A
10. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
11. Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A
Post Your Comments:
(Date:9/15/2014)... State University have developed a technique for controlling the ... voltages, opening the door to a new generation of ... hinges on the fact that the oxide "skin" of ... acts as a surfactant, lowering the surface tension ... researchers used a liquid metal alloy of gallium and ...
(Date:9/15/2014)... MORRISVILLE, NC (PRWEB) September 15, 2014 ... newly developed deep imaging OCT system for the optical ... , The EnvisuTM S4410 SDOCT for Contact ... measurements for standard and high complexity contact lens, IOL ... allows imaging of a complete contact lens immersed in ...
(Date:9/15/2014)... The chemistry, taste, and health effects of tea can vary ... the nonprofit American Botanical Council (ABC). Recent research by ... on the phytochemical compounds in tea ( Camellia sinensis ) ... in the Yunnan province of southwestern ... future of medicinal botanicals. Dr. Ahmed,s report on ...
(Date:9/15/2014)... septiembre de 2014 La segunda anual International ... 12 al 18 de octubre. Como iniciativa conjunta de la ... compañías miembro s, la IPAW se ha diseñado para: ... a la colección de fuentes de plasma , Reconocimiento ... y mejorar las vidas , Aumento del conocimiento sobre ...
Breaking Biology Technology:Researchers control surface tension to manipulate liquid metals 2Bioptigen Introduces Deep Imaging OCT System for Contact Lens Metrology 2Tea Taste and Quality Affected by Climate Change 2Tea Taste and Quality Affected by Climate Change 3Tea Taste and Quality Affected by Climate Change 4La International Plasma Awareness Week será una celebración para los donantes 2La International Plasma Awareness Week será una celebración para los donantes 3
... Ondine Biopharma,Corporation (the "Company" or "Ondine", TSX: ... photodisinfection based products, today,announced its financial results ... 2008., "During the past quarter we ... photodisinfection technology and we are leveraging our,successes ...
... 14 Palatin Technologies,Inc. (Amex: PTN ) ... clinical,trial of PL-3994, a novel, long-acting natriuretic peptide ... heart failure (HF). The,Phase 2a trial was a ... volunteers with controlled hypertension who,received the medication or ...
... professor of aerospace and ocean engineering at Virginia Tech, ... E. Powe Junior Faculty Award. The award will support ... are new material and structural systems that have the ... "I believe these innovative materials will lead to new ...
Cached Biology Technology:Ondine Biopharma Announces Second Quarter 2008 Financial Results 2Ondine Biopharma Announces Second Quarter 2008 Financial Results 3Ondine Biopharma Announces Second Quarter 2008 Financial Results 4Ondine Biopharma Announces Second Quarter 2008 Financial Results 5Ondine Biopharma Announces Second Quarter 2008 Financial Results 6Ondine Biopharma Announces Second Quarter 2008 Financial Results 7Ondine Biopharma Announces Second Quarter 2008 Financial Results 8Ondine Biopharma Announces Second Quarter 2008 Financial Results 9Ondine Biopharma Announces Second Quarter 2008 Financial Results 10Ondine Biopharma Announces Second Quarter 2008 Financial Results 11Ondine Biopharma Announces Second Quarter 2008 Financial Results 12Ondine Biopharma Announces Second Quarter 2008 Financial Results 13Ondine Biopharma Announces Second Quarter 2008 Financial Results 14Ondine Biopharma Announces Second Quarter 2008 Financial Results 15Ondine Biopharma Announces Second Quarter 2008 Financial Results 16Ondine Biopharma Announces Second Quarter 2008 Financial Results 17Palatin Technologies, Inc. Reports Positive Results of Phase 2a Clinical Study with PL-3994 for the Treatment of Heart Failure 2Palatin Technologies, Inc. Reports Positive Results of Phase 2a Clinical Study with PL-3994 for the Treatment of Heart Failure 3
(Date:9/15/2014)... PULLMAN, Wash.Washington State University researchers have found "the most ... that can be used to transfer valuable genes ... the way for breeders to develop wheat varieties with ... a legion of genetic tools that can reduce crop ... hurdles and controversy of Genetically Modified Organisms, or GMOs., ...
(Date:9/15/2014)... understand how the fish communities might shift under ... of environmental data to inform future decisions relating ... said Paula Whitfield, a research ecologist at NOAA,s ... lead author of the study. , The North ... where historically, both temperate and tropical species live, ...
(Date:9/15/2014)... the Department of Science and Technology in Society in ... Virginia Tech, has won a 2014 National Science Foundation ... and problems of creating a global nuclear emergency response ... Nuclear Power Plant in March 2011 was a turning ... Schmid said. Three of the plant,s six nuclear reactors ...
Breaking Biology News(10 mins):WSU researchers find 'most famous wheat gene' 2Study finds warming Atlantic temperatures could increase range of invasive species 2'Nuclear disasters don't respect national boundaries' 2
... German . Scientists at the Max Planck ... insights for stem cell research which are also applicable to ... treatments. As Rolf Kemler,s research group discovered, a molecular link ... telomeres and a signalling pathway known as the Wnt/β-signalling pathway. ...
... smaller than the nano as it is the equivalent of ... green algae of this imperceptible size existing in the Bilbao ... estuary. This has enabled him to identify six genera and ... been catalogued in these waters. He has also put forward ...
... toward treating disease with minute capsules containing not drugs ... making the drug. In an article in ACS, journal ... nano-sized capsules that contain the genetically coded instructions, plus ... that can be switched on with an external signal. ...
Cached Biology News:A nanoscopic look at the estuary's green algae 2A nanoscopic look at the estuary's green algae 3
Immunogen: Secretory component isolated from human colostrum. Storage: 4 C...
... PinPoint™ Vector Sequencing Primer is designed for ... Vectors (Cat.# V2031, V2051, V2061). The primer ... at nucleotides 325343, approximately 4050 base pairs ... can be used to determine if an ...
Negative Control, eluted and packaged aseptically in 20l of 10mM Tris-HCL, pH 8.0, 1 mM EDTA, 5 years at either 4°C or -20°C...
Medium for cryopreserving subcutaneous or omental preadipocytes...
Biology Products: