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Comparison of Growth Techniques and Media for the Purpose of Plasmid ,,, Isolation from E. coli Using the Eppendorf ,,, Perfectprep Plasmid Mini ,,, Kit

ors and shakers.

Plasmid DNA Quantity Purified from Bacterial Cultures

Plasmid Yield

Figure 2. Comparison of plasmid yields in the different media and culture vessels.

Conclusions

After data collection and analysis of a series of experiments comparing bacterial growth in different media using 15 ml culture tubes versus the Eppendorf LidBac, it can be concluded that the Eppendorf Lidion with the Thermomixer R and a rich media is a more efficient and effective method for growing small volumes of bacteria.

References

  1. Eppendorf Thermomixer R and 2.0 ml Thermoblock, Eppendorf AG
  2. LidBac tubes, Eppendorf AG
  3. Deardorff MA, Tan C, Conrad LJ and Klein PS. Frizzled-8 is expressed in the Spemann organizer and plays a role in early morphogenesis. Development. 1998;125:2687-2700.
  4. Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd ed. 1989;3:A1-A3.
  5. ibid.
  6. ibid.
  7. BioPhotometer, Eppendorf AG
  8. Molecular Biology Grade Water, Eppendorf AG


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