Comparison of Growth Techniques and Media for the Purpose of Plasmid ,,, Isolation from E. coli Using the Eppendorf ,,, Perfectprep Plasmid Mini ,,, Kit
of Bacterial Cultures in 15 ml Conical Tubes and LidBac
Figure 1. Comparison of E. coli cultivation in LB, 2X YT and
TB mediums using 15 ml tubes and LidBac. A. Growth curves of bacterial
cultures in LB media. B. Growth curves of bacterial cultures in 2X YT
media. C. Growth curves of bacterial cultures in TB media.
In the experiments shown, the culture was allowed to proceed to the log
phase. Figure 1 shows a comparison of cultures grown in 15 ml culture
tubes and the Eppendorf LidBac in three different media. In all cases,
growth curves of bacterial cultures were higher in the samples grown in
LidBac. Clearly, the cultures experienced better growth when LidBac was
used as the culture vessel, regardless of the media.
The table in Figure 2 demonstrates that the growth rates achieved were
directly proportional to the yield of plasmid DNA after purification.
A 1.4- to 3-fold increase is seen in these samples, depending on the media
used. As expected, the greatest differences were seen in the samples grown
in TB, which also had the greatest differences in growth curves (Figure
One of the critical factors related to the difference in growth rates
is the superior air exchange characteristic of the hydrophobic 0.22 m
membrane of LidBac. In contrast, the 15 ml culture tubes require loose
capping to prevent contamination, thus limiting air exchange within the
tube. Increased aeration and improved growth rates seen with the LidBac
culture can also be attributed to the increased agitation rate that can
be achieved with the Thermomixer R compared to conventional incubatPage: All 1 2 3 4 Related biology technology :1
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