Navigation Links
Comparison of Growth Techniques and Media for the Purpose of Plasmid ,,, Isolation from E. coli Using the Eppendorf ,,, Perfectprep Plasmid Mini ,,, Kit

Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit

Joel Lopez, Melissa Higgins, Chere Griffin and Vince Prezioso, PhD
BioSystems Laboratory, Brinkmann Instruments, Inc., Westbury, New York

Introduction

Isolation and purification of plasmid DNA from bacteria involves three steps: growth of the bacterial culture, harvesting and lysis of the bacteria, and purification of plasmid DNA. Growth of the bacterial cultures is a key factor in obtaining high quality DNA. Using Eppendorfs Thermomixer R in conjunction with Eppendorfs LidBac is an ideal combination.1,2 The LidBac provides excellent gas interchange through a 0.22 m hydrophobic membrane while preventing contamination. The LidBac also allows for growth in standard microcentrifuge tubes, enhanced by excellent heat transmission of the Thermomixer R and suitable Thermoblock.

Objectives

This paper will present an alternative method of growing bacteria in a more reliable manner and at a lower overall cost due to the direct use of the culture vessel in the isolation procedure. The LidBac and Thermomixer R is a dependable combination that provides researchers with an easy-to-use system to grow bacterial cultures directly on the bench top in small quantities.

Materials and Methods

Cell Cultivation and Culture Medias

Escherichia coli (E. coli) strain DH5a containing Plasmid pCS2++ were inoculated into 1.5 ml of LB (Luria-Bertani Medium), TB (Terrific Broth) and 2X YT and cultivated in membrane
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
2. Performance Comparisons of Commercial RT-PCR Systems
3. A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7 Cell Lines
4. TripleMaster PCR System: Direct Comparison with Competitors
5. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
6. Comparison of different protein quantitation methods
7. Comparison of Different Methods of mRNA Quantitation
8. Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A
9. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
10. Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A
11. Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach
Post Your Comments:
(Date:7/11/2014)... 2014 Research and Markets  has announced ... - Global Industry Analysis, Size, Share, Growth, Trends and ... http://photos.prnewswire.com/prnh/20130307/600769 Transfection ... DNA or RNA) into cells. This technology helps the ... and protein metabolism by affecting the nuclear genes. The ...
(Date:7/11/2014)... 11, 2014  Researchers have pioneered a revolutionary ... new technology, called Virtual Finger, allows scientists to ... neurons and synapses using the flat surface of ... 3D imaging studies orders of magnitude more efficient, ... level across many areas of experimental biology. The ...
(Date:7/10/2014)... (PRWEB) July 10, 2014 Deep ... Century Medicine Forum on ‘Commercialising Longevity Research’ and ... entrepreneurs to the London Bioscience Innovation Center for ... Foundation and Aging Analytics Ltd. The meeting highlighted ... in translational research for age-related disease, as well ...
(Date:7/10/2014)... Robert Harman, DVM, Founder and CEO of Vet-Stem, ... announce the relaunch of his highly informative blog, now named ... “ What are Stem Cells ?” Dr. Harman’s purpose ... in the basics of stem cell therapy so that pet ... of treatment when considering regenerative medicine. , A veterinarian by ...
Breaking Biology Technology:International Transfection Technology Market - Industry Analysis, Size, Share, Growth, Trends and Forecast to 2019 2Virtual Finger Enables Scientists To Navigate And Analyze 3D Images Of Complex Biological Structures 2Virtual Finger Enables Scientists To Navigate And Analyze 3D Images Of Complex Biological Structures 3DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 2DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 3DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 4DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 5DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 6Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 2Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 3
... Amarin Corporation plc,(NASDAQ: AMRND ) today announced that, ... 1 for 10 reverse split of each of its ... close of business,yesterday, January 17, 2008., Thomas Lynch, ... pleased to report that over 95% of the votes ...
... ... now means that all the population is susceptible to ... the disease, - Increased concerns over "second wave" of vCJD in individuals with much ... longer incubation times - P-Capt(R) prion reduction filter still the only proven method of protecting ...
... Calif. University of California, Irvine researchers have identified ... cerebral cortex, a finding that could lead to stem ... as stroke and Alzheimers. , Dr. Edwin Monuki, doctoral ... of Pathology & Laboratory Medicine and Developmental & Cell ...
Cached Biology Technology:Amarin Provides Business Update and Announces Implementation of Reverse Stock Split 2Amarin Provides Business Update and Announces Implementation of Reverse Stock Split 3Amarin Provides Business Update and Announces Implementation of Reverse Stock Split 4Amarin Provides Business Update and Announces Implementation of Reverse Stock Split 5Amarin Provides Business Update and Announces Implementation of Reverse Stock Split 6Amarin Provides Business Update and Announces Implementation of Reverse Stock Split 7Amarin Provides Business Update and Announces Implementation of Reverse Stock Split 8Amarin Provides Business Update and Announces Implementation of Reverse Stock Split 9ProMetic and MacoPharma announce successful completion of P-Capt(R) prion reduction filter clinical studies 2ProMetic and MacoPharma announce successful completion of P-Capt(R) prion reduction filter clinical studies 3ProMetic and MacoPharma announce successful completion of P-Capt(R) prion reduction filter clinical studies 4ProMetic and MacoPharma announce successful completion of P-Capt(R) prion reduction filter clinical studies 5Discovery of 'creator' gene for cerebral cortex points to potential stem cell treatments 2
(Date:7/11/2014)... Shenzhen, China Researchers from Salk Institute for Biological ... time evaluated the safety and reliability of the ... a new method, TALEN-HDAdV, which could significantly increased ... (hiPSC). This study published online in Cell ... for stem cell-based gene therapy. , The combination ...
(Date:7/11/2014)... Researchers have pioneered a revolutionary new way ... called Virtual Finger, allows scientists to move through ... synapses using the flat surface of their computer ... studies orders of magnitude more efficient, saving time, ... many areas of experimental biology. The software and ...
(Date:7/11/2014)... is evidence that under the normal circumstances, ... development, maintain neuronal environment, and exhibit therapeutic ... neurodegenerative disease. Previous studies have found that ... after chemical induction have reduced viability, which ... Prof. Xiaodong Yuan, Kailuan General Hospital, Hebei ...
Breaking Biology News(10 mins):A new genome editing method brings the possibility of gene therapies closer to reality 2Virtual finger enables scientists to navigate and analyze complex 3D images 2
... 4, 2013   MedNet Solutions , a global life ... is pleased to announce its sponsorship of, participation in, ... 10-13 in Chicago .  MedNet is ... conference...the largest and most prestigious clinical data management event ...
... anti-cancer drugs and other medicines inside soil microbes and other ... given humanity more than 50 percent of today,s prescription drugs. ... cover story in Chemical & Engineering News , the ... Jarvis, C&EN senior editor, points out that natural products are ...
... useful medical information beyond what current newborn screening already ... being funded by the Eunice Kennedy Shriver National Institute ... National Human Genome Research Institute (NHGRI), both parts of ... to four grantees have been made in fiscal year ...
Cached Biology News:MedNet Solutions To Sponsor The SCDM Annual Conference For The Eighth Straight Year 2NIH program explores the use of genomic sequencing in newborn healthcare 2NIH program explores the use of genomic sequencing in newborn healthcare 3NIH program explores the use of genomic sequencing in newborn healthcare 4