Navigation Links
Comparison of Growth Techniques and Media for the Purpose of Plasmid ,,, Isolation from E. coli Using the Eppendorf ,,, Perfectprep Plasmid Mini ,,, Kit

Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit

Joel Lopez, Melissa Higgins, Chere Griffin and Vince Prezioso, PhD
BioSystems Laboratory, Brinkmann Instruments, Inc., Westbury, New York

Introduction

Isolation and purification of plasmid DNA from bacteria involves three steps: growth of the bacterial culture, harvesting and lysis of the bacteria, and purification of plasmid DNA. Growth of the bacterial cultures is a key factor in obtaining high quality DNA. Using Eppendorfs Thermomixer R in conjunction with Eppendorfs LidBac is an ideal combination.1,2 The LidBac provides excellent gas interchange through a 0.22 m hydrophobic membrane while preventing contamination. The LidBac also allows for growth in standard microcentrifuge tubes, enhanced by excellent heat transmission of the Thermomixer R and suitable Thermoblock.

Objectives

This paper will present an alternative method of growing bacteria in a more reliable manner and at a lower overall cost due to the direct use of the culture vessel in the isolation procedure. The LidBac and Thermomixer R is a dependable combination that provides researchers with an easy-to-use system to grow bacterial cultures directly on the bench top in small quantities.

Materials and Methods

Cell Cultivation and Culture Medias

Escherichia coli (E. coli) strain DH5a containing Plasmid pCS2++ were inoculated into 1.5 ml of LB (Luria-Bertani Medium), TB (Terrific Broth) and 2X YT and cultivated in membrane
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
2. Performance Comparisons of Commercial RT-PCR Systems
3. A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7 Cell Lines
4. TripleMaster PCR System: Direct Comparison with Competitors
5. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
6. Comparison of different protein quantitation methods
7. Comparison of Different Methods of mRNA Quantitation
8. Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A
9. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
10. Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A
11. Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach
Post Your Comments:
(Date:5/28/2015)... May 28, 2015 BioNorth Texas ... and representing the life sciences industry in northern ... Directors meeting at The Foundry in Dallas on ... organization brought together a broad spectrum of biotech ... the organization's early successes in partnering with key ...
(Date:5/28/2015)... MARLBOROUGH, Mass. , May 28, 2015 ... company focused on discovering and developing innovative therapeutics, primarily ... the pricing of a public offering of 26 million ... per share for gross proceeds of approximately $10.4 million. ... stock, (ii) a 13-month overallotment purchase right to purchase ...
(Date:5/28/2015)... 2015  GenVec, Inc. (Nasdaq: GNVC ) today ... Swirsky , will present a company overview at the ... on Thursday, June 4, 2015. The ... be available at the following link:  http://wsw.com/webcast/jeff88/gnvc .  ... To access, visit www.genvec.com , click on "Investors ...
(Date:5/28/2015)... The Fertility Centers of New England is proud to ... on a new and expanded role as Medical Director. ... oversight and quality assurance for the practice. , “We ... of experienced reproductive endocrinologists,” said Fertility Centers of New ... expertise and compassionate, individualized care have helped set FCNE ...
Breaking Biology Technology:BioNorthTX Holds Inaugural Board of Directors Meeting 2BioNorthTX Holds Inaugural Board of Directors Meeting 3RXi Pharmaceuticals Announces $10 Million Public Offering of Common Stock and Warrants 2RXi Pharmaceuticals Announces $10 Million Public Offering of Common Stock and Warrants 3GenVec To Present At The Jefferies Healthcare Conference 2Danielle Vitiello, MD of Fertility Centers of New England, Named New Medical Director 2
... store all the films ever made on a device the ... few metallic atoms could make it possible to build radically ... components for spintronics devices. There,s just one obstacle on the ... temperatures a few hairs above absolute zero. Now a chemistry ...
... Ga., March 28, 2011 MiMedx Group, Inc.  (OTC ... manufacturer and marketer of patent protected biomaterial-based products and ... results for the year ended December 31, 2010. ... changed its fiscal year to coincide with the calendar ...
... DRIVE4COPD, the nation,s single largest awareness campaign for ... Healthcare Campaign of the Year 2011.  The ... honors in the public relations industry, given each year ...  Boehringer Ingelheim Pharmaceuticals, Inc. is the founding sponsor of ...
Cached Biology Technology:Heavy metals open path to high temperature nanomagnets 2MiMedx Group Announces 2010 Results 2MiMedx Group Announces 2010 Results 3MiMedx Group Announces 2010 Results 4MiMedx Group Announces 2010 Results 5MiMedx Group Announces 2010 Results 6MiMedx Group Announces 2010 Results 7DRIVE4COPD Wins PRWeek Healthcare Campaign of the Year 2011 2DRIVE4COPD Wins PRWeek Healthcare Campaign of the Year 2011 3DRIVE4COPD Wins PRWeek Healthcare Campaign of the Year 2011 4
(Date:4/27/2015)... 27, 2015 NXT-ID, Inc. (NASDAQ: NXTD ... focused on the growing mobile commerce market, announces that ... customers the first week of May, 2015 and will ... May. Gino Pereira , Chief ... the company as Wocket® enters the consumer market. We ...
(Date:4/21/2015)... High crime rate, increasing ... access control systems market in Saudi Arabia ... published report by TechSci Research " Saudi Arabia Access Control ... systems market in Saudi Arabia ... control systems market in the Kingdom is growing at ...
(Date:4/20/2015)... April 20, 2015 The announcement ... Glenbeigh Records Management (GRM), Ireland,s ... Islamic Bank. Having built up an impressive track record of ... leading player within the records management sector in ... double is GCC staffbase and employ a further eight staff ...
Breaking Biology News(10 mins):Wocket, the Smartest Wallet You Will Ever Own, Announces Shipment to Pre-order Customers 2Wocket, the Smartest Wallet You Will Ever Own, Announces Shipment to Pre-order Customers 3Saudi Arabia Access Control Systems Market to Touch USD1.2 Billion by 2020 Says TechSci Research 2Saudi Arabia Access Control Systems Market to Touch USD1.2 Billion by 2020 Says TechSci Research 3Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 2Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 3
... A tissue-repair-and-regeneration pathway in the human body, including wound ... properly. Genetically engineered mice fail to develop lungs ... are knocked out. The findings are described this week ... know the answer to a seemingly simple question: What ...
... and city officials recently signed a memorandum of understanding ... temperate rainforest education and research center in Juneau. The ... will stimulate and develop temperate rainforest education and research. ... of the University of Alaska Southeast, University of Alaska ...
... NOAA Fisheries scallop survey off the northeastern U.S. coast ... "recruit" sea scallops and ocean quahogs on Georges Bank ... Mid-Atlantic for 2009. This survey, which included the first ... underwater imaging system from the same vessel, also shows ...
Cached Biology News:How to make a lung 2New collaborative center to provide education, research on temperate rainforests 2Annual survey shows high numbers of seed scallops on Georges Bank, low numbers in Mid-Atlantic 2Annual survey shows high numbers of seed scallops on Georges Bank, low numbers in Mid-Atlantic 3Annual survey shows high numbers of seed scallops on Georges Bank, low numbers in Mid-Atlantic 4
... Luminescent Detection System is intended for use ... generated by an in-house validated method for ... selected target of interest. • ... is a hybridization capture assay designed to ...
... for cell culture scale-up, these culture chambers ... - 10 9 range and are ... spinner flasks. • High mechanical • strength ... prior to shipping • Certified nonpyrogenic and ...
Mouse monoclonal [7B9] to beta II Tubulin ( Abpromise for all tested applications). Antigen: Synthetic peptide, CEEEEGEDEA found at the c-terminus of beta II tubulin. Entrez GeneID: 10383 ...
...
Biology Products: