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Comparison of Different Methods of mRNA Quantitation

Tolerates Partially Degraded RNA
Yes
No
Yes
Yes
Advantages
  • Can use up to 100 g of sample RNA
  • Ease of multiprobing
  • Tolerates partially degraded RNA
  • RNA undergoes minimal processing
  • Technique can be easily evaluated at various points in procedure
  • Most sensitive technique available
  • Localized mRNA within tissues
  • Tolerates partially degraded RNA

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Preparation Note: Prepared by method of Wimmer, E., et al., Biochemistry, 7, 2623 (1968). Quality Clarifier: Acceptor Activity: approx. 1000 picomoles phenylalanine per A 260 unit (not determined b...
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