Navigation Links
Comparison of Different Methods of mRNA Quantitation


FeatureNuclease Protection AssaysNorthern BlottingRT-PCRIn Situ HybridizationRelative and Absolute Quantitation
Yes
Yes
Yes
Yes
Detection Limit (Copies of mRNA)
4,000-5,000
10,000
1 (theoretical)
Medium
Level of Optimization Required
Medium
Medium
High
High
Sample Size
100 g max
30 g max
Not critical
Tissue sections
Detect Multiple Transcripts
Yes, in same tube
Yes, ge nerally requires stripping and reprobing
Yes, in same tube
No
Localizes mRNA Expression within Tissues/Cells
No
No
No
Yes
Mapping Studies
Yes
No
No
No
Resolve Comigrating mRNAs
Yes
No
Yes
No
Sizing mRNAs
No
Yes
No
No
Detection of Alternatively Spliced Transcripts
No
Yes
No
No
Distinguish Between Members of Multi-Gene Families
Yes
Only if they are different sizes
Only if they are characterized
No
Tolerates Partially Degraded RNA
Yes
No
Yes
Yes
Advantages
  • Can use up to 100 g of sample RNA
  • Ease of multiprobing
  • Tolerates partially degraded RNA
  • RNA undergoes minimal processing
  • Technique can be easily evaluated at various points in procedure
  • Most sensitive technique available
  • Localized mRNA within tissues
  • Tolerates partially degraded RNA

'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
2. Performance Comparisons of Commercial RT-PCR Systems
3. A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7 Cell Lines
4. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
5. TripleMaster PCR System: Direct Comparison with Competitors
6. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
7. Comparison of different protein quantitation methods
8. Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A
9. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
10. Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A
11. Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:7/14/2017)... ... July 14, 2017 , ... Sonic Manufacturing Technologies ... Technologies has installed a solar system on its roof top. “We will be ... of Sonic, Kenneth Raab stated. The company’s proud history of social responsibility and ...
(Date:7/14/2017)... Denville, USA/ Martinsried, Germany (PRWEB) , ... July ... ... a newly developed standardized solution for sample preparation of proteins to clean peptides ... the Diagenode Bioruptor® sonication system. , The PreOmics iST Kit is based ...
(Date:7/13/2017)... , ... July 13, 2017 , ... ... surgical instruments in Orthopedics, has announced today the completion of a major transaction ... to drive organic and external growth. The alliance fuels In’Tech Medical’s service offerings ...
(Date:7/13/2017)... , ... July 13, 2017 , ... ... programs at Philadelphia’s University City Science Center, Christopher Laing, MRCVS, Ph.D. has been ... Austin, Texas. Dr. Laing will become the first Executive Director at the ...
Breaking Biology Technology:
(Date:4/17/2017)... Florida , April 17, 2017 NXT-ID, ... technology company, announces the filing of its 2016 Annual Report on ... and Exchange Commission. ... on Form 10-K is available in the Investor Relations section of ... as on the SEC,s website at http://www.sec.gov . ...
(Date:4/11/2017)... PALM BEACH GARDENS, Fla. , April 11, ... biometric identity management and secure authentication solutions, today ... million contract by Intelligence Advanced Research Projects Activity ... technologies for IARPA,s Thor program. "Innovation ... the onset and IARPA,s Thor program will allow ...
(Date:4/11/2017)... , April 11, 2017 NXT-ID, ... security technology company, announces the appointment of independent Directors Mr. ... to its Board of Directors, furthering the company,s corporate ... ... NXT-ID, we look forward to their guidance and benefiting from ...
Breaking Biology News(10 mins):