Navigation Links
Comparing Fidelity and Performance of Proofreading PCR Enzymes

itor recently introduced a new proofreading PCR enzyme, called PLATINUM Pfx DNA polymerase. Contrary to its trade name, Pfx was derived from the Pyrococcus sp. strain KOD1 (P. sp. KOD) rather than Pyrococcus furiosus (Pfu), the source of Pfu DNA polymerase. KOD DNA polymerase was first described by Tagaki, et al.6, and commercialized for PCR by Toyobo. Phylogenetic analyses have shown that, although reportedly from a Pyrococcus isolate,6 KOD DNA polymerase is more closely related to enzymes from Thermococcus species (e.g., Vent, 9N-7) than Pyrococcus species (Pfu, Deep Vent).7 According to its manufacturer, the Pfx version of KOD DNA polymerase possesses hot start capability provided by neutralizing polymerase antibodies.

The manufacturers claimed that Pfx DNA polymerase exhibits greater accuracy than Pfu DNA polymerase8 (authors cite Tagaki, et al.6). Pfx was also reported to provide comparable sensitivity and higher amplification specificity compared to PfuTurbo DNA polymerase.8 In this report, we present the results of direct fidelity and PCR performance comparisons between PfuTurbo DNA polymerase and Pfx DNA polymerase. PCR performance is assessed with respect to product yield, sensitivity, specificity, and target-length capability.

PCR Fidelity Comparisons

PCR enzyme fidelity has been measured using a number of different methods, including DGGE analyses3 and monitoring phenotypic changes in mutational target genes (lacI1 and p53 2). These studies showed that Pfu DNA polymerase exhibits the lowest intrinsic error rate of any commercial thermostable DNA polymerase. For example, using a PCR mutation assay based upon the lacIOlacZa target gene,1 Pfu DNA polymerase exhibited an average error rate 2-fold lower than that of Vent and Deep Vent DNA polymerases,1 3- to 6-f
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
2. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
3. High-Fidelity PCR with a Novel Polymerase Mixture
4. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
5. Expand High FidelityPLUS PCR System
6. Expand High Fidelity PCR System
7. High Fidelity PCR Master
8. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
9. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
10. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
11. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Comparing Fidelity and Performance Proofreading PCR Enzymes

(Date:6/18/2013)... Tabletop SEMs are inexpensive and easy to ... performance and capability such as small sample sizes, lower ... provide better imaging performance and more analytical capability but ... higher cost of maintenance. The Pemtron PS-230 and ... types of SEM product, offering competitive prices compared with ...
(Date:6/18/2013)... 18, 2013 (PRWEB) June 18, 2013 ... attendance system in North America, has introduced AccountLinx ... data and payroll records between ExakTime’s software and ... automates ExakTime software setup and keeps employee databases ... data from a business’ current payroll software, directly ...
(Date:6/18/2013)... June 18, 2013 DuPont welcomes 48 ... 11th annual DuPont National AgriScience Teachers Ambassador Academy this ... in Chestertown, Md. , The NATAA “Ag ... and is a special project of the National ... Agricultural Educators (NAAE) . The highly recommended agriscience ...
(Date:6/18/2013)... information — Global biopharmaceutical company UCB, Inc. and professional ... of a new Pledge campaign for people with epilepsy ... empowers the millions of Americans living with epilepsy ... to demand more: greater understanding of the condition, better ... seizure control.  More information is available at www.ourtimeourpledge.com ...
Breaking Biology Technology:Nanounity Introduces the Pemtron Range of Compact Scanning Electron Microscopes 2Nanounity Introduces the Pemtron Range of Compact Scanning Electron Microscopes 3ExakTime Introduces AccountLinx Plus 2DuPont Sponsors National AgriScience Ambassador Academy 2DuPont Sponsors National AgriScience Ambassador Academy 3Professional Golfer Jeff Klauk Tees Off New Pledge Campaign for People Living with Epilepsy 2Professional Golfer Jeff Klauk Tees Off New Pledge Campaign for People Living with Epilepsy 3Professional Golfer Jeff Klauk Tees Off New Pledge Campaign for People Living with Epilepsy 4Professional Golfer Jeff Klauk Tees Off New Pledge Campaign for People Living with Epilepsy 5Professional Golfer Jeff Klauk Tees Off New Pledge Campaign for People Living with Epilepsy 6
... China, April 4, 2008 /Xinhua-PRNewswire-FirstCall/ -- WuXi,PharmaTech ... PharmaTech"), a leading,pharmaceutical, biotechnology and medical device ... China and the United States, today,announced that ... Form F-1 with the,Securities and Exchange Commission ...
... April 4, 2008 Scientists in fields,as diverse ... from the Olympus FluoroPoint(TM) single molecule fluorescence,detection system ... of laboratories around the world, the system, with ... of use, is now,available for the first time ...
... (Nasdaq: IDMI ) announced today the appointment of ... 3, 2008. Mr. Tibbitts is,currently the chief financial officer ... medical device company and in this role has overseen ... as from a clinical,stage company to the current commercial ...
Cached Biology Technology:WuXi PharmaTech (Cayman) Inc. Announces Filing of Registration Statement For Follow-On and Secondary Offering 2Olympus FluoroPoint(TM) System Provides High-Sensitivity Single Molecule and Protein Interaction Analysis for Screening and Research 2Olympus FluoroPoint(TM) System Provides High-Sensitivity Single Molecule and Protein Interaction Analysis for Screening and Research 3IDM Pharma Appoints Gregory J. Tibbitts to Board of Directors 2IDM Pharma Appoints Gregory J. Tibbitts to Board of Directors 3
(Date:6/17/2013)... French . A ... Alzheimer,s disease can be reversed with treatment. The study by ... Neuro, at McGill University and at Universit de Montral found ... brain of mice with advanced Alzheimer,s disease (AD) recovers memory ... of Neuroinflammation in May, also suggest an underlying mechanism ...
(Date:6/16/2013)... together and move around the body called ,chase and ... scientists at UCL. , Published in Nature Cell ... occurs when cancer cells interact with healthy cells in order ... cancer cells recruit healthy cells and use them to travel ... it could be controlled to design new therapies against cancer ...
(Date:6/14/2013)... students have difficulty understanding and explaining how evolution ... the subject, researchers at Michigan State University developed ... the molecular changes underlying an evolving characteristic to ... researchers, Peter J. T. White, Merle K. Heidemann, ... the scenarios into a cellular and molecular biology ...
Breaking Biology News(10 mins):Blocking overactive receptor in Alzheimer's recovers memory loss and more 2'Chase and run' cell movement mechanism explains process of metastasis 2Teaching complete evolutionary stories increases learning 2
... Biotechnology and Biological Sciences Research Council (BBSRC) at the University ... explain why the earliest evidence of complex multicellular animal life ... on the planet rose sharply from 3% to their modern ... Chris Schofield, has found that humans share a method of ...
... indicates an increased risk for developing breast cancer can ... according to a new study featuring work from researchers ... and their colleagues. The multidisciplinary team found that ... more likely to be present in breast cancer patients ...
... 2010) Whitehead Institute Founding Member Rudolf Jaenisch has been ... Massachusetts General Hospital (MGH). By tradition, the ... the prize with Dr. Shinya Yamanaka, who is on the ... Institutes in San Francisco. "The Warren Prize is the ...
Cached Biology News:550 million years ago rise in oxygen drove evolution of animal life 2Key information about breast cancer risk and development is found in 'junk' DNA 2Key information about breast cancer risk and development is found in 'junk' DNA 3Whitehead Member Rudolf Jaenisch to receive MGH's Warren Triennial Prize 2
Mouse polyclonal antibody to MCFD2 - multiple coagulation factor deficiency 2...
Anti (porcine) Vitronectin...
... to work in collaboration with Pall Life ... filters in a variety of membrane chemistries. ... use on the Caliper TPWII, MultiDose, Prelude, ... for our Automation Certified Syringe Filters are ...
... to BMP2 BMP2 belongs to ... superfamily. Bone morphogenic protein induces bone ... for the autosomal dominant disease of ... Immunogen: Synthetic peptide: PSDEVLSEFELRLLSM conjugated ...
Biology Products: