( ous amounts (in ng) of human genomic DN...)Combine High Yield, Great Accuracy and the,,,Prevention of Carry-over Contamination by Using,,,the Novel Expand High FidelityPLUS PCR System

ous amounts (in ng) of human genomic DNA were used to amplify a 4.8 kb fragment from the tissue plasminogen activator (tPA) gene. The manufacturers recommended reaction conditions were strictly followed. The result shows that Expand HiFiPLUS produced the best sensitivity, specificity, and yield, even from as little as 1 ng human genomic DNA.



Avoid Cross-Contamination and Obtain Reliable Results

Cross-contamination is a common problem in every lab. Until now, carry-over prevention via the incorporation of dUTP and subsequent treatment with Uracil-DNA Glycosylase was only possible with Taq DNA Polymerase for fragments up to 2.5 kb. The Expand HiFiPLUS Enzyme blend now enables the incorporation of dUTP in fragments up to 5 kb. This makes Expand HiFiPLUS the enzyme of choice for high-fidelity, highyield amplification of longer fragments.



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