Navigation Links
Combine High Yield, Great Accuracy and the,,,Prevention of Carry-over Contamination by Using,,,the Novel Expand High FidelityPLUS PCR System

Roche Applied Science pioneered the blending of thermostable DNA polymerases and the associated buffer systems. The well-known Expand High Fidelity and Expand Long Template PCR Systems were the first commercially available blends to provide amplification of fragments up to 5kb and 20 kb, respectively. The Expand High FidelityPLUS PCR System is a next-generation blend consisting of Taq DNA Polymerase and a novel proofreading protein, isolated and characterized by Roche Applied Science. This protein mediates proofreading activity but has no polymerase activity itself.

The synergy between the mechanism of the proofreading protein and the processivity of Taq DNA Polymerase is the key to Expand High FidelityPLUS Enzyme Blends ability to
  • deliver high yield combined with improved accuracy
  • incorporate dUTP to prevent carry-over contamination when performing amplifications up to 5 kb.
The combination of these characteristics makes the Expand HiFiPLUS Enzyme Blend the product of choice for the simultaneous amplification of a larger number of targets.

Various thermostable DNA polymerases as individual enzymes or blends offer good yield or accuracy. However, the Expand HiFiPLUS Enzyme Blend is the only formulation that delivers high yield and great fidelity for amplification of fragments up to 5 kb.

Expand the Performance of PCRs up to 5 kb

Expand HiFiPLUS Enzyme Blend successfully challenged four products from different suppliers, and proved to be the blend delivering the best sensitivity and specificity for the amplification of fragments up to 5 kb (Figure). Vari ous amounts (in ng) of human genomic DNA were used to amplify a 4.8 kb fragment from the tissue plasminogen activator (tPA) gene. The manufacturers recommended reaction conditions were strictly followed. The result shows that Expand HiFiPLUS produced the best sensitivity, specificity, and yield, even from as little as 1 ng human genomic DNA.

Avoid Cross-Contamination and Obtain Reliable Results

Cross-contamination is a common problem in every lab. Until now, carry-over prevention via the incorporation of dUTP and subsequent treatment with Uracil-DNA Glycosylase was only possible with Taq DNA Polymerase for fragments up to 2.5 kb. The Expand HiFiPLUS Enzyme blend now enables the incorporation of dUTP in fragments up to 5 kb. This makes Expand HiFiPLUS the enzyme of choice for high-fidelity, highyield amplification of longer fragments.



Page: All 1 2

Related biology technology :

1. Greater Amplification Specificity with New Hot Start PCR Enzyme
2. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
3. Enjoy a Greater Sense of Security with PCR-Grade Water
4. 96 Well Pipetting Precision and Accuracy Using the RapidPlate 96/384 Workstation
5. 384 Well Pipetting Precision and Accuracy Testing Using the Sciclone Advanced Liquid Handler
6. Preventing Carry-over Contamination with Uracil-DNA Glycosylase
7. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
8. Combat RNase Contamination in the Lab
9. The Fastest, Simplest, and Most Effective Way to Remove DNA Contamination
10. Testing Compressed Air Lines for Microbiological Contamination
11. Testing Compressed Air Lines for Microbiological Contamination
Post Your Comments:

(Date:6/23/2016)... ... 2016 , ... Mosio, a leader in clinical research patient ... and Retention Tips.” Partnering with experienced clinical research professionals, Mosio revisits the hurdle ... and strategies for clinical researchers. , “The landscape of how patients receive and ...
(Date:6/23/2016)... 2016 /PRNewswire/ - FACIT has announced the creation ... biotechnology company, Propellon Therapeutics Inc. ("Propellon" or "the ... a portfolio of first-in-class WDR5 inhibitors for the ... WDR5 represent an exciting class of therapies, possessing ... for cancer patients. Substantial advances have been achieved ...
(Date:6/23/2016)... ... June 23, 2016 , ... ... and Mold) microbial test has received AOAC Research Institute approval 061601. , “This ... introduced last year,” stated Bob Salter, Vice President of Regulatory and Industrial Affairs. ...
(Date:6/23/2016)... , June 23, 2016   EpiBiome , ... secured $1 million in debt financing from Silicon Valley ... up automation and to advance its drug development efforts, ... new facility. "SVB has been an incredible ... the services a traditional bank would provide," said Dr. ...
Breaking Biology Technology:
(Date:6/2/2016)... 2, 2016 The Department of Transport ... the 44 million US Dollar project, for the , ... including Personalization, Enrolment, and IT Infrastructure , to ... production and implementation of Identity Management Solutions. Numerous renowned international ... Decatur was selected for the most compliant ...
(Date:6/2/2016)... , June 2, 2016 Perimeter ... Platforms, Unmanned Systems, Physical Infrastructure, Support & Other Service  ... visiongain offers comprehensive analysis of the global ... market will generate revenues of $17.98 billion in 2016. ... DVTEL Inc, a leader in software and hardware technologies ...
(Date:5/24/2016)... Ampronix facilitates superior patient care by providing unparalleled technology to leaders of the medical ... premium product recently added to the range of products distributed by Ampronix. ... ... ... Ampronix News ...
Breaking Biology News(10 mins):