Navigation Links
Combine High Yield, Great Accuracy and the,,,Prevention of Carry-over Contamination by Using,,,the Novel Expand High FidelityPLUS PCR System

Roche Applied Science pioneered the blending of thermostable DNA polymerases and the associated buffer systems. The well-known Expand High Fidelity and Expand Long Template PCR Systems were the first commercially available blends to provide amplification of fragments up to 5kb and 20 kb, respectively. The Expand High FidelityPLUS PCR System is a next-generation blend consisting of Taq DNA Polymerase and a novel proofreading protein, isolated and characterized by Roche Applied Science. This protein mediates proofreading activity but has no polymerase activity itself.

The synergy between the mechanism of the proofreading protein and the processivity of Taq DNA Polymerase is the key to Expand High FidelityPLUS Enzyme Blends ability to
  • deliver high yield combined with improved accuracy
  • incorporate dUTP to prevent carry-over contamination when performing amplifications up to 5 kb.
The combination of these characteristics makes the Expand HiFiPLUS Enzyme Blend the product of choice for the simultaneous amplification of a larger number of targets.

Various thermostable DNA polymerases as individual enzymes or blends offer good yield or accuracy. However, the Expand HiFiPLUS Enzyme Blend is the only formulation that delivers high yield and great fidelity for amplification of fragments up to 5 kb.

Expand the Performance of PCRs up to 5 kb

Expand HiFiPLUS Enzyme Blend successfully challenged four products from different suppliers, and proved to be the blend delivering the best sensitivity and specificity for the amplification of fragments up to 5 kb (Figure). Vari
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Greater Amplification Specificity with New Hot Start PCR Enzyme
2. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
3. Enjoy a Greater Sense of Security with PCR-Grade Water
4. 96 Well Pipetting Precision and Accuracy Using the RapidPlate 96/384 Workstation
5. 384 Well Pipetting Precision and Accuracy Testing Using the Sciclone Advanced Liquid Handler
6. Preventing Carry-over Contamination with Uracil-DNA Glycosylase
7. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
8. Combat RNase Contamination in the Lab
9. The Fastest, Simplest, and Most Effective Way to Remove DNA Contamination
10. Testing Compressed Air Lines for Microbiological Contamination
11. Testing Compressed Air Lines for Microbiological Contamination
Post Your Comments:
(Date:9/1/2015)... MILWAUKEE , Sept. 1, 2015 ... Eric Floyd , Ph.D., MBA as Chief ... (DLSS).  In this newly created role, Dr. Floyd ... well as a portfolio of pre-commercial and post ... medical affairs and medical communications.  Dr. Floyd brings ...
(Date:9/1/2015)... Sept. 1, 2015   Medfusion, Inc., ... relationship between doctors and patients, today announced a ... N.C. -based venture capital firms.   ... association with Bull City Venture Partners and Hatteras ... Founder and Executive Chairman. "Both firms, experience working ...
(Date:9/1/2015)... ... September 01, 2015 , ... Research ... California Los Angeles, Boston University School of Medicine, Frederick National Laboratory for Cancer ... the world’s first multidisciplinary Open Access journal. , Christopher Ryan PhD, of ...
(Date:8/31/2015)... (PRWEB) , ... August 31, 2015 , ... ... year to bring together supply chain risk management and resiliency practitioners with luminaries ... and share best practices, and be recognized for their innovations and performance excellence. ...
Breaking Biology Technology:Dohmen Life Science Services Appoints Eric Floyd, Ph.D. as Chief Science Officer 2Dohmen Life Science Services Appoints Eric Floyd, Ph.D. as Chief Science Officer 3Medfusion Secures Funding to Continue Evolution and Advance Mobile Strategy 2Medfusion Secures Funding to Continue Evolution and Advance Mobile Strategy 3Synedgen’s Director for Pharmaceutical Manufacturing Publishes New Research 2Synedgen’s Director for Pharmaceutical Manufacturing Publishes New Research 3Global Supply Chain Resiliency Council Announces Incorporation and Web Site Launch 2Global Supply Chain Resiliency Council Announces Incorporation and Web Site Launch 3
... July 21, 2011 The U.S. Food & Drug ... a clinical trial for gene therapy for a rare ... and London, this trial based on preclinical research performed ... gene therapy product, is now going to be launched ...
... July 21, 2011 Advanced Cell Technology, Inc. ("ACT"; ... medicine, announced today that it has entered into an ... Biotech"), a leading South Korea-based biotechnology company, designed to ... venture, Stem Cell & Regenerative Medicine International (SCRMI).  Under ...
... DURHAM, N.C. Researchers at Duke University Medical Center ... of Chlamydia , the bacteria responsible for the ... States. The team, which included Duke University ... the bacteria,s self-defense mechanisms. The therapies that ...
Cached Biology Technology:Genethon and Children's Hospital Boston Get FDA approval for a Wiskott Aldrich Gene Therapy Trial 2Genethon and Children's Hospital Boston Get FDA approval for a Wiskott Aldrich Gene Therapy Trial 3Genethon and Children's Hospital Boston Get FDA approval for a Wiskott Aldrich Gene Therapy Trial 4Genethon and Children's Hospital Boston Get FDA approval for a Wiskott Aldrich Gene Therapy Trial 5ACT Obtains Exclusive License for Hemangioblast Technology in North America 2ACT Obtains Exclusive License for Hemangioblast Technology in North America 3ACT Obtains Exclusive License for Hemangioblast Technology in North America 4ACT Obtains Exclusive License for Hemangioblast Technology in North America 5Newly designed molecule blocks chlamydia bacteria 2Newly designed molecule blocks chlamydia bacteria 3
(Date:8/28/2015)... -- According to a new market research ... (Pen & Paper Based, Hosted, Biometrics), Service, Application (Clinical ... Vertical and Region - Global Forecast to 2020", published ... from USD 2.4 Billion in 2015 to USD 7.5 ... (CAGR) of 25.6% from 2015 to 2020. ...
(Date:8/24/2015)... August 24, 2015 The consulting company ... largest biometrics manufacturer DERMALOG and its customized solutions and products ... Biometrics Company of the Year Award". DERMALOG is particularly successful ... .   -Cross reference: Picture is available at ... On Thursday evening, in South Africa,s ...
(Date:8/20/2015)... 20, 2015   Sensory, Inc ., a ... user experience and security of consumer electronics through ... that its TrulySecure™ is the first ... be FIDO Certified™. The FIDO (Fast ... the FIDO UAF (Universal Authentication Framework) 1.0 specifications, ...
Breaking Biology News(10 mins):Cognitive Assessment and Training Market Worth 7.5 Billion USD by 2020 2Cognitive Assessment and Training Market Worth 7.5 Billion USD by 2020 3Cognitive Assessment and Training Market Worth 7.5 Billion USD by 2020 4Frost & Sullivan Honors DERMALOG with Biometrics Prize for Achievements in Africa 2Frost & Sullivan Honors DERMALOG with Biometrics Prize for Achievements in Africa 3TrulySecure From Sensory Becomes First Face and Voice Biometrics Technology to be FIDO UAF Certified 2TrulySecure From Sensory Becomes First Face and Voice Biometrics Technology to be FIDO UAF Certified 3TrulySecure From Sensory Becomes First Face and Voice Biometrics Technology to be FIDO UAF Certified 4TrulySecure From Sensory Becomes First Face and Voice Biometrics Technology to be FIDO UAF Certified 5
... STATION Researchers at Texas A&M University have confirmed for ... off the Texas coast for at least the past 23 ... to marine life in the area. Steve DiMarco, associate ... dead zones in the Gulf of Mexico for more than ...
... fresh evidence that urbanization in the United States threatens the ... six-year study also refutes one of the most widely accepted ... kinds of birds. Most ecologists have assumed that common ... and raccoons were destroying eggs or killing young birds ...
... Md., April 1, 2008 The U.S. Pharmacopeial (USP) ... dissolution variability of USP Prednisone Lot P Reference Standard ... less variability in USP Prednisone Lot P tablets than ... dosage forms plays a critical role in drug manufacturing ...
Cached Biology News:Researchers confirm dead zone off Texas coast since 1985 2Some migratory birds can't find success in urban areas, study finds 2Some migratory birds can't find success in urban areas, study finds 3Prednisone tablets less variable than marketed drugs 2
... Specificity: This antibody recognizes murine ... cross-react with other related IRF ... with murine IRF7 protein was ... of the 293T cells transfected ...
... PS603. Immunogen: Synthetic phosphopeptide ... the phosphorylated Ser603 residue of ... Specific for synapsin I when ... Reactivity: Rat (positive control: ...
MMP-20 Immunogen: Activated recombinant human MMP-2 Storage: -20 C, Avoid Freeze/Thaw Cycles...
karyopherin alpha2 (2G7)...
Biology Products: