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Combat RNase Contamination in the Lab

than those experienced under normal use.


Detection
Sensitive Detection of RNases
If you need to detect RNases, then RNaseAlert is the kit of choice. This patent-pending technology reports RNase activity in a convenient and sensitive assay that delivers fast, accurate results in real time. The RNaseAlert Kit uses a novel RNA substrate tagged with a fluorescent reporter molecule (fluor) on one end and a quencher on the other. In the absence of RNases, the physical proximity of the quencher dampens fluorescence from the fluor to extremely low levels. When RNases are present, however, the RNA substrate is cleaved and the fluor and quencher are spatially separated in solution. This causes the fluor to emit a bright green signal (when excited by light of the appropriate wavelength) that can be readily detected by eye (with illumination on a UV box) or with a filter-based or monochromator-based fluorometer. The sequence of the RNaseAlert Substrate has been carefully optimized for sensitive detection of several RNases, including RNase A, RNase T1, RNase I, micrococcal nuclease, S1 nuclease, mung bean nuclease, and Benzonase.

RNaseAlert is provided in two convenient kit formats. The RNaseAlert Lab Test Kit is suitable for testing small sample numbers and can be used to ensure that solutions, tubes, tips, etc. are RNase-free. The RNaseAlert QC System is designed for
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