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Combat RNase Contamination in the Lab

ted nucleases and either SUPERaseIn or RI. Both the SUPERaseIn and the RI were added at a concentration of 1 U/l.


The most widely used RNase inhibitor is placental ribonuclease inhibitor (RI), sold as Ribonuclease Inhibitor Protein. SUPERaseIn is distinct from RI in that it has a more robust interaction with RNases, does not require DTT for activity, and inhibits a broader range of RNases. While placental ribonuclease inhibitor is effective only against RNase A-type enzymes (e.g., RNase A, B, and C), SUPERaseIn also inhibits RNase I and T1. SUPERaseIn does not interfere with other enzymes such as RNA polymerases, reverse transcriptase or Taq polymerase. Additionally, SUPERaseIn is active up to 65C and at pH 5.58.5.


RNase-free Buffers and Reagents
It is essential that any buffer or reagent added to purified RNA be free of RNase contamination. A simple alternative to the tedious preparation of RNase-free solutions is to purchase certified RNase-free water, buffers, and reagents such as those offered by Ambion. After carefully isolating the RNA, it is crucial that the pellet be resuspended and stored in an RNase-free solution. Ambion's molecular biology reagents are tested under extremely stringent conditions for consistent lot-to-lot quality. Our quality control philosophy is to subject our reagent products to conditions 10-100 times more stringent
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