( ted nucleases and either SUP...)Combat RNase Contamination in the Lab

The most widely used RNase inhibitor is placental ribonuclease inhibitor (RI), sold as Ribonuclease Inhibitor Protein. SUPERaseIn is distinct from RI in that it has a more robust interaction with RNases, does not require DTT for activity, and inhibits a broader range of RNases. While placental ribonuclease inhibitor is effective only against RNase A-type enzymes (e.g., RNase A, B, and C), SUPERaseIn also inhibits RNase I and T1. SUPERaseIn does not interfere with other enzymes such as RNA polymerases, reverse transcriptase or Taq polymerase. Additionally, SUPERaseIn is active up to 65C and at pH 5.58.5.

RNase-free Buffers and Reagents
It is essential that any buffer or reagent added to purified RNA be free of RNase contamination. A simple alternative to the tedious preparation of RNase-free solutions is to purchase certified RNase-free water, buffers, and reagents such as those offered by Ambion. After carefully isolating the RNA, it is crucial that the pellet be resuspended and stored in an RNase-free solution. Ambion's molecular biology reagents are tested under extremely stringent conditions for consistent lot-to-lot quality. Our quality control philosophy is to subject our reagent products to conditions 10-100 times more stringent
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