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Combat RNase Contamination in the Lab


Trace amounts of ribonuclease (RNase) contamination can sabotage experiments. Since RNases are ubiquitous in the laboratory environment--on your skin, in the air, on anything touched by bare hands or on anything left open to the air--it is important to make an effort to prevent or eliminate RNase contamination. However, determining which piece of equipment or solution is responsible for RNase contamination can be frustrating and time-consuming. Ambion provides a complete arsenal of products to help prevent, detect, and eliminate RNase contamination in molecular biology experiments.


Prevention
SUPERaseIn: The Ultimate RNase Inhibitor
SUPERaseIn RNase Inhibitor (patent pending) is a protein-based inhibitor of nonhuman origin that noncovalently binds and inhibits the most common and troublesome RNases including RNase A, B, C, 1 and T1. SUPERaseIn can be used in any application where RNase contamination could be problematic. It is ideal for in vitro transcription and translation, cDNA synthesis, RT-PCR, and preparation of RNase-free antibodies. Because it inhibits a broader range of RNases than traditional RNase inhibitors, SUPERaseIn is the most effective RNase inhibitor available, providing a higher level of protection against degradation (Figure 1).


Figure 1. SUPERaseIn vs. Placental Ribonuclease Inhibitor (RI). A 32P-labeled RNA probe was incubated for 30 minutes at 37C in the presence of the indica
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An excellent carbonyl-reactive FRET quencher paired with Trp or Tyr. Minimum Purity: 95%. Abs(max): ~350 nm; Em(max): none. Solvent System:DMSO or DMF....
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