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Codon Bias-Adjusted BL21 Derivatives for Protein Expression

Jun-N-terminal kinase (JNK) or l-phosphatase, that are well expressed in conventional BL21 cells, were indistinguishable between the two strains (Figure 1).

Conclusions

Stratagenes new BL21-CodonPlus series of competent cells allows high-level expression of recombinant genes that are restricted in their expression in conventional E. coli due to codon usage bias. Many proteins can be expressed in BL21-CodonPlus-RIL cells instead of moving into a different expression system.

Acknowledgements

The authors thank Alan Greener, Connie Hansen, and Xiuyuan Hu.

REFERENCES

  1. Jerpseth, M., et al. (1998) Strategies 11: 3-4.

  2. Kane, J.F. (1995) Curr. Opin. Biotechnol. 6: 494-500.

  3. Forman, M.D., et al. (1998) Protein Sci. 7: 500-503.

  4. Hu, X., et al. (1996) Protein Expr. Purif. 7: 289-293.

* Patent pending
** Patents pending


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