Navigation Links
Codon Bias-Adjusted BL21 Derivatives for Protein Expression

justed hcTnT-mut in conventional BL21-Gold(DE3) cells. Hence, using BL21-CodonPlus(DE3)-RIL strains are as efficient as employing the conventional and time-consuming method of synonymous replacement of rare codons to achieve high-level expression of codon-usage restricted recombinant genes.

Fig.2

The simultaneous expression of several rare tRNA genes in BL21-CodonPlus(DE3)-RIL not only provides a generic host for expression of recombinant genes affected by a range of rare codons but is also necessary for some recombinant genes to achieve a high level of expression. In Figure 2, using an archaeal gene as an example, efficient production of this recombinant gene in E. coli hosts requires the simultaneous presence of functional extra copies of both argU and ileY tRNA genes. Variants of the BL21-CodonPlus(DE3)-RIL strains lacking either the argU or ileY gene, while retaining the other tRNA genes, failed to produce the target gene at the same level as observed in the BL21-CodonPlus(DE3)-RIL strain. The functional expression argU and ileY genes in each of thevariant strains were confirmed using expression constructs, which are dependent only on either argU function (hcTnT) or ileY function (3xi) for efficient expression. The artificial construct 3xi is derived from a well-expressed recombinant gene by insertion of three AUA codons (ile) at the N-terminus.

The host cells did not suffer any obvious deleterious effects from the extra copies of tRNA genes. Growth rates and transformation efficiencies were comparable to the parental BL21-Gold cells. When comparing BL21-CodonPlus(DE3)-RIL cells with BL21-Gold(DE3) cells, production of recombinant genes like
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
2. New BL21-CodonPlus Cells Correct Codon Bias in GC-Rich Genomes
3. High-efficiency Derivatives of the BL21 Series for Protein Expression
4. A New C-Terminal GST Vector for Protein Production in S. pombe
5. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
6. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
7. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
8. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
9. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
10. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
11. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Codon Bias Adjusted Derivatives for Protein Expression

(Date:8/20/2014)... analysis of patented university inventions licensed to biotechnology ... to commercialization. To open these roadblocks, the researchers ... during the discovery stage could lead to faster ... frequently derived from discoveries made in university laboratories ... known during clinical trials, which have a high ...
(Date:8/20/2014)... High performance computing (HPC) will be ... climate and Earth system model to address the ... Eight national laboratories, including Lawrence Livermore , ... Atmospheric Research, four academic institutions and one private-sector ... laboratories include Argonne, Brookhaven, Lawrence Berkeley, Los Alamos, ...
(Date:8/20/2014)... August 20, 2014 Not long ago, ... studies should be performed late in the development cycle ... But current regulatory trends suggest that, like many things ... rise. In recent years, multiple drug sponsors have been ... address specific questions during phase I/II. It’s not only ...
(Date:8/20/2014)... GraphDB™ 6.0 from ... the enterprise replication cluster, faster loading speeds, higher ... Elasticsearch. This release happens to coincide with a ... GraphDB™ was formerly known as OWLIM. ... enterprise resilient RDF triplestore will also benefit from ...
Breaking Biology Technology:Early bottlenecks in developing biopharmaceutical products delay commercialization 2Early bottlenecks in developing biopharmaceutical products delay commercialization 3New Project Is the ACME of Addressing Climate Change 2New Project Is the ACME of Addressing Climate Change 3Are Extractable and Leachables going Phase-Appropriate? New Webinar Hosted by Xtalks 2Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 2Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 3Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 4
... Robots today are making a considerable impact on ... to healthcare. Reaching for the human frontier, robotics ... of new emerging domains. Interacting, exploring, and working ... increasingly touch peoples lives. The prospect of practical ...
... DIEGO, May 19 Gen-Probe Incorporated,(Nasdaq: GPRO ... renowned,molecular microbiologist at Stanford University, has been elected ... eight members, including,seven who are not Gen-Probe employees., ... intelligence and enthusiasm,to an already strong Gen-Probe board, ...
... meat and pork processing ... ... Corp.,(OTC Bulletin Board: TKER), a distributor and marketer of proprietary,technology effective ... the Food Safety and Inspection,Service ("FSIS") of the United States Food ...
Cached Biology Technology:Robots moving closer to humans 2Lucy Shapiro, Ph.D., Elected to Gen-Probe Board of Directors 2Lucy Shapiro, Ph.D., Elected to Gen-Probe Board of Directors 3Tasker Products Receives Authorization from USDA for its Tasker Clear for use in Meat and Pork Industries 2Tasker Products Receives Authorization from USDA for its Tasker Clear for use in Meat and Pork Industries 3
(Date:8/20/2014)... scientists have discovered that acral melanomas the rare type ... are genetically distinct from other more common types of ... in the journal Pigment Cell & Melanoma Research . ... hands, soles of the feet, nail-beds and other hairless parts ... it,s not caused by UV damage from the sun. , ...
(Date:8/20/2014)... Virginia Tech,s Wu Feng has built upon a National ... in the Cloud" program, and synergistically complemented it with ... the Air Force on "big computing" for mini-drones and ... Institutes of Health on "big data" for the life ... aspects from each grant, he was able to tell ...
(Date:8/20/2014)... a colony of honeybees grows to about 4,000 members, ... cycle: the building of a special type of comb ... of experts from the Department of Neurobiology and Behaviour ... starts the reproductive cycle of honeybee colonies. The results ... Science of Nature . , Reproduction isn,t always ...
Breaking Biology News(10 mins):Scientists learn more about rare skin cancer that killed Bob Marley 2The internet was delivered to the masses; parallel computing is not far behind 2The internet was delivered to the masses; parallel computing is not far behind 3The internet was delivered to the masses; parallel computing is not far behind 4Worker bees 'know' when to invest in their reproductive future 2
... vibrating in place or taking part in protein folding ... Simulating molecular motions provides researchers with information critical to ... certain diseases, such as Alzheimer,s and Parkinson,s, that result ... researchers needed either supercomputers or large computer clusters to ...
... FALLS CHURCH, Va., Feb. 2 CSC (NYSE: ... of 12 contractors awarded a Biometrics Operations and Support ... Army Biometrics Task Force (BTF). The indefinite-delivery/indefinite-quantity (IDIQ) contract ... orders to meet the mission needs of BTF business ...
... million cancer survivors living in the United States are ... to the cost, and Hispanics and African-Americans are twice ... presented at the American Association for Cancer Research conference ... "These survivors are either going without, or significantly delaying, ...
Cached Biology News:New open-source software permits faster desktop computer simulations of molecular motion 2New open-source software permits faster desktop computer simulations of molecular motion 3CSC Awarded Department of Defense Biometrics Contract 2More than a million cancer survivors declining care due to cost concerns 2
... The Thermo Sequenase Dye Primer Manual Cycle ... with fluorescent dye-labeled primers and high-resolution fluorescence ... followed by standard slab gel electrophoresis and ... tagged sequencing products. The kit components ...
... agitation and temperature control for microbiology and ... independently and is suitable for DASGIP bioreactors ... volume. Its temperature ranges from approximately 10 ... is equipped with an individual electrical heating ...
... SYBR Green II RNA gel stain ... that has bright fluorescence when bound to RNA ... for use with either formaldehyde/agarose or polyacrylamide gels ... µL stains 50 minigels. A 1 mL unit ...
... the data from any one of IITC NIBP ... The final data is sent from the amplifier ... automatic for Systolic, Mean, Heart Rate while the ... always in the position to override the automatic ...
Biology Products: