Navigation Links
Codon Bias-Adjusted BL21 Derivatives for Protein Expression

10.9

18.4

6.0

9.8

12.6

5.2

Although strains of the BL21-Gold series are the most convenient and effective bacterial hosts for protein production presently available, expression of heterologous recombinant genes in E. coli is frequently limited when the codon usage in the recombinant gene differs from host cells (Table 1). Forced high-level expression of a gene, with codons that are rarely used by E. coli, results in depletion of the internal tRNA pools.2 As a consequence, translation of the recombinant RNA species is delayed, leading to its enhanced degradation. The result of rare codon usage is most frequently seen as ineffective to nondetectable production of the target protein and the formation of aborted translation products that have the appearance of proteolytic degradation. In addition, rare codons may also affect the quality of the produced proteins by inducing translation frameshifts, codon skipping, and frequent misincorporation of amino acids. Misincorporation rates of up to 40% have been reported at specific rare arginine codons.3

The deleterious effects of rarely used codons have been most thoroughly documented for the arginine codons AGA and AGG, which are the rarest codons in E. coli. However, codons for isoleucine (AUA), leucine (CUA), and proline (CCC) have also been demonstrated to affect protein production and quality in E. coli hosts. The expression of affected recombinant genes can be salvaged either by synonymous replacement of the rare codons with more frequently used codons u
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
2. New BL21-CodonPlus Cells Correct Codon Bias in GC-Rich Genomes
3. High-efficiency Derivatives of the BL21 Series for Protein Expression
4. A New C-Terminal GST Vector for Protein Production in S. pombe
5. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
6. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
7. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
8. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
9. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
10. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
11. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Codon Bias Adjusted Derivatives for Protein Expression

(Date:7/10/2014)... sit upside down forever. Nanophysics makes sure of that. Mechanics ... just published in Physical Review E . Knowledge that ... that seem to be able to sit still forever, and ... years. We will soon be able to buy smart new ... But the fact is, sooner or later the grip is ...
(Date:7/10/2014)... alliance will see the two universities and NPL collaborate ... business and industry and their complementary academic strengths., Working ... to shape the scientific priorities of the UK. The ... to 300 high-calibre PhD students, will provide a pipeline ... the potential of the Laboratory will be grown through ...
(Date:1/15/2014)... 2014  Bill Jacobs Automotive, a group of car dealerships headquartered ... the Heartland Blood Center and offering free oil change coupons ... place Saturday, Jan. 18 at the Bill Jacobs Cadillac and ... (Photo: http://photos.prnewswire.com/prnh/20140115/MN46637 )  The Bill Jacobs ...
(Date:1/15/2014)... 2014 Look inside the new Preferred ... the lab, from fluid handling to instruments to supplies. ... when you order. , Preferred Solutions features a ... the L/S® model for precise flow control and dispensing ...
Breaking Biology Technology:Even geckos can lose their grip 2Universities of Surrey and Strathclyde selected as strategic partners in the future operation of the National Physical Laboratory 2Universities of Surrey and Strathclyde selected as strategic partners in the future operation of the National Physical Laboratory 3Join the Bill Jacobs Auto blood drive and receive a free oil change 2Cole-Parmer Begins 2014 with the Release of Preferred Solutions 2
... ... ... ... ...
... ... ... ... ...
... ... ... ... ...
Cached Biology Technology:Citius Tech Launches BI-Clinical MUSE for Advanced "Meaningful Use" Reporting and Data Submission, and Performance Management for Healthcare ISVs 2Citius Tech Launches BI-Clinical MUSE for Advanced "Meaningful Use" Reporting and Data Submission, and Performance Management for Healthcare ISVs 3Citius Tech Launches BI-Clinical MUSE for Advanced "Meaningful Use" Reporting and Data Submission, and Performance Management for Healthcare ISVs 4Elsevier Addresses Today's Healthcare Information Crisis With a Renewed Focus on Improving Quality and Efficiency 2Elsevier Addresses Today's Healthcare Information Crisis With a Renewed Focus on Improving Quality and Efficiency 3Elsevier Addresses Today's Healthcare Information Crisis With a Renewed Focus on Improving Quality and Efficiency 4Elsevier Addresses Today's Healthcare Information Crisis With a Renewed Focus on Improving Quality and Efficiency 5Elsevier Addresses Today's Healthcare Information Crisis With a Renewed Focus on Improving Quality and Efficiency 6Elsevier Addresses Today's Healthcare Information Crisis With a Renewed Focus on Improving Quality and Efficiency 7Elsevier Addresses Today's Healthcare Information Crisis With a Renewed Focus on Improving Quality and Efficiency 8Elsevier Addresses Today's Healthcare Information Crisis With a Renewed Focus on Improving Quality and Efficiency 9Peer-reviewed Articles Further Define Clusterin and the Role of a Clusterin Inhibitor, OGX-011, for the Treatment of Cancer 2Peer-reviewed Articles Further Define Clusterin and the Role of a Clusterin Inhibitor, OGX-011, for the Treatment of Cancer 3Peer-reviewed Articles Further Define Clusterin and the Role of a Clusterin Inhibitor, OGX-011, for the Treatment of Cancer 4Peer-reviewed Articles Further Define Clusterin and the Role of a Clusterin Inhibitor, OGX-011, for the Treatment of Cancer 5Peer-reviewed Articles Further Define Clusterin and the Role of a Clusterin Inhibitor, OGX-011, for the Treatment of Cancer 6Peer-reviewed Articles Further Define Clusterin and the Role of a Clusterin Inhibitor, OGX-011, for the Treatment of Cancer 7
(Date:7/11/2014)... the brains of all vertebrates, information is transmitted ... or chemical signal to be passed from one ... the most abundant type of synapse, can be ... for learning, memory, perception and cognition, and the ... brain function. For instance, every time we ...
(Date:7/10/2014)... YORK , June 27, 2014  Pomerantz ... Provectus Biopharmaceuticals, Inc. ("Provectus" or the "Company")(NYSE-MKT: PVCT) ... filed in United States District Court, Middle District ... is on behalf of a class consisting of ... acquired Provectus securities between December 17, 2013 and ...
(Date:7/10/2014)... , July 2, 2014  Unisys Corporation,s (NYSE: ... Unisys Nederland N.V., today announced that it has been selected ... and manage a new Basic Provision Biometrics solution for penitentiaries ... Unisys won the contract with a solution based on its ... The term of the contract is up to seven years, ...
Breaking Biology News(10 mins):Blame it on the astrocytes 2SHAREHOLDER ALERT: Pomerantz Law Firm Announces the Filing of a Class Action against Provectus Biopharmaceuticals, Inc. and Certain Officers - PVCT 2SHAREHOLDER ALERT: Pomerantz Law Firm Announces the Filing of a Class Action against Provectus Biopharmaceuticals, Inc. and Certain Officers - PVCT 3Unisys Delivers New Basic Provision Biometrics Solution to Dutch Custodial Institutions Service 2Unisys Delivers New Basic Provision Biometrics Solution to Dutch Custodial Institutions Service 3
... Friday, October 7, 2011, Cleveland: Researchers have ... diseases like asthma, rheumatoid arthritis, psoriasis, inflammatory bowel ... this pathway may provide opportunities for tailored treatments ... of this pathway was the work of an ...
... researchers from the University of Modena and Reggio Emilia ... that in aphids the two X chromosomes have a different ... . Aphids are insects with a sex determination ... in females and a single X chromosome (XO) in males. ...
... Biological invasions, i.e. the spread of introduced, non-native species, ... bring out the importance of economic activities on ecological ... variety of the interaction of economics with invasion science ... problems. Three researchers, lead by Mark Williamson from ...
Cached Biology News:Chromosome inheritance? Not the same for all the chromosomes 2
... Mouse monoclonal [6331] to Salmonella typhimurium ... family Enterobacteriaceae. The genus is composed of Gram-negative ... possess 3 major antigens; the "H" or flagellar ... somatic antigen (part of the LPS moiety) and ...
... mg/L sodium pyruvate, 50 mg/L streptomycin sulfate, ... calcium chloride, and magnesium chloride. The use ... tissue culture is generally attributed to early ... Ringer developed a solution of inorganic salts ...
... 1 is a dramatic breakthrough in accurate, small-volume ... 10nL resolution, the micro 1 has unique advantages ... the micro 1 never needs re-calibration as do ... programming on an LCD Display , Graphical Row ...
... Rabbit polyclonal to Rad51 ( ... Antigen: Synthetic ... to N terminal amino acids 15-29 ... Entrez Gene ID: 5888 ...
Biology Products: