Navigation Links
Codon Bias-Adjusted BL21 Derivatives for Protein Expression


High-level expression of difficult heterologous proteins

Carsten-Peter Carstens Anna Waesche
Stratagene

Stratagene has created variants of the Epicurian Coli BL21-Gold cells * called the BL21-CodonPlus -RIL series** of strains, which contain extra copies of the E. coli argU, ileY, and leuW tRNA genes. This modification allows for a high-level expression of proteins that are difficult to express in conventional E. coli hosts due to the codon bias of the gene of interest. The BL21-CodonPlus-RIL strains can eliminate the need to move the gene of interest into eukaryotic expression systems.

BL21-Gold(DE3) cells are the strain of choice for high-level production of heterologous proteins expressed from the T7-promoter. These strains have been engineered to contain an inducible T7-RNA polymerase. They are also EndA- to allow high-quality miniprep DNA preparation.1 BL21 competent cells are derivatives of E. coliB and lack the Lon protease and the ompT protease genes that may cause instability and degradation of the recombinant protein.

Table 1
Rarest Codons (from E. coli) Preferred by Selected Organisms

Codon frequencies are expressed as codons used per 1000 codons encountered. A complete compilation of codon usage of the sequences placed in the gene bank database can be found at http://www.dna.affrc.go.jp/~nakamura/codon.html Codon frequencies of more than 15 codons/1000 codons are shown in bold to help identify a codon bias that may cause problems for high-level expression in E. coli. The arginine codons AGG and AGA are recognized by the same tRNA (pr
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
2. New BL21-CodonPlus Cells Correct Codon Bias in GC-Rich Genomes
3. High-efficiency Derivatives of the BL21 Series for Protein Expression
4. A New C-Terminal GST Vector for Protein Production in S. pombe
5. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
6. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
7. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
8. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
9. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
10. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
11. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Codon Bias Adjusted Derivatives for Protein Expression

(Date:12/22/2014)... Dec. 22, 2014  ( www.competitivehealth.com ) — Competitive ... medical bill review and advocacy service, has signed an ... WellCard Savings discount health services marketplace. 63% ... more than they expected to pay. As part of ... costs, WellCard Savings is pleased to offer medical bill ...
(Date:12/22/2014)... 22, 2014 Fertility Associates of Memphis ... Bank USA , a network of more than 20 ... partnership, patients have fast access to a national pool ... IVF . Fertility Associates of Memphis is the only ... treatment option. , Dr. William Kutteh , director ...
(Date:12/19/2014)... BioPlus Specialty Pharmacy (BioPlus), one ... of Nick Maroulis, Pharm.D. to the newly created position ... In this position, Dr. Maroulis will continue to manage ... of our multi-site pharmacies as the company expands. Dr. ... that time he has served in many different capacities ...
(Date:12/19/2014)... Dec. 19, 2014 Bina Technologies, Inc. (Bina, ... that they have been acquired by Roche (SIX: RO, ... privately held company that provides a big data platform ... (NGS) data for the academic and translational research markets. ... and will continue to focus on development of their ...
Breaking Biology Technology:WellCard Savings Announces CoPatient Medical Bill Review Service for Members 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 3BioPlus Specialty Pharmacy Promotes Dr. Nick Maroulis to Vice President of Specialty Pharmacy Services 2Bina Technologies is acquired by Roche. 2Bina Technologies is acquired by Roche. 3
... Aligns Operations for Enhanced Performance, DECATUR, ... (NYSE: ADM ) chairman, chief executive ... organizational restructure of the,Company, designed to enhance ... responsibility for long-term success. The reorganization creates ...
... oximetry technology to significantly reduce, false alarms without missing ... ... Aug. 22 Masimo (Nasdaq:,MASI), the inventor of Pulse CO-Oximetry ... partnership with Apria,Healthcare to provide pulse oximetry products and accessories ...
... symbol: SBS, - Canadian Biotechnology Company confirms commercial ... AI Milano variant and clarifies status of native ... - SemBioSys Genetics Inc.,(TSX:SBS), a biotechnology company developing ... diseases, today updated a release,issued on July 12, ...
Cached Biology Technology:ADM Reorganizes Operations 2ADM Reorganizes Operations 3ADM Reorganizes Operations 4Apria Healthcare Chooses Masimo SET Pulse Oximetry for Use in the Company's Home Health Care Business 2Apria Healthcare Chooses Masimo SET Pulse Oximetry for Use in the Company's Home Health Care Business 3SemBioSys updates Apo AI development program 2SemBioSys updates Apo AI development program 3SemBioSys updates Apo AI development program 4
(Date:12/11/2014)... 2014  That blood pressure plays a role in human ... the medical term for high blood pressure – was first ... inflatable cuff that,s used in measuring blood pressure was invented ... new about hypertension, its triggers and its effects. In fact, ... and the best ways to treat it. For ...
(Date:12/10/2014)... 9, 2014  Valencell, a leader in performance biometric ... staggering demand from its licensees for highly accurate, clinically ... not solely coming from fitness and health sectors, but ... "A wearable is only as useful as the biometric ... ultimate driver in long-term mass consumer adoption of wearable ...
(Date:12/10/2014)... Research and Markets ( http://www.researchandmarkets.com/research/xclcvw/biometrics_market ... "Biometrics Market in Japan 2014-2018" report to ... The integration of biometrics and ... and upgradation of the driver,s license is one ... Besides the aforementioned projects, biometrics is being used ...
Breaking Biology News(10 mins):Research points to need for new approaches to treatment of high blood pressure 2Research points to need for new approaches to treatment of high blood pressure 3Research points to need for new approaches to treatment of high blood pressure 4Wearable Technology Products Demand Highly Accurate Biometric Technology 2Biometrics Market in Japan 2014-2018: Key Vendors are DDS, Fujitsu, Hitachi and NEC 2
... sole proprietors of their nests, some tropical species time ... a 150-year-old evolutionary mystery, says Queens University Biology professor ... confirms one of Charles Darwins more controversial theories ... many experts that different species can arise, unhindered, ...
... The Shifting Baselines Toolset is presented to the public and ... , When: Public symposium 1pm to 5:15pm, Wednesday 14th November ... city campus. , The meeting will be launched with a ... for interviews on the 14th and on other days by ...
... 13, 2007) A major study that will provide a ... be conducted by the La Jolla Institute for Allergy ... The five-year study, funded by the National Institute ... National Institutes of Health, could lead to revolutionary new ...
Cached Biology News:'Time-sharing' tropical birds key to evolutionary mystery 2How much fish have we got? 2How much fish have we got? 3NIH selects LIAI for major study on allergy molecular causes and possible treatments 2NIH selects LIAI for major study on allergy molecular causes and possible treatments 3NIH selects LIAI for major study on allergy molecular causes and possible treatments 4
Human ROR1 Affinity Purified Polyclonal Ab...
Ultra-sensitive, non-radioactive measurement of COX-I and COX-II activity...
Phospho-p70 S6 Kinase (T229) Affinity Purified PAb...
Recombinant Ovine (Sheep) Growth Hormone, Ultra Pure...
Biology Products: