Navigation Links
Codon Bias-Adjusted BL21 Derivatives for Protein Expression


High-level expression of difficult heterologous proteins

Carsten-Peter Carstens Anna Waesche
Stratagene

Stratagene has created variants of the Epicurian Coli BL21-Gold cells * called the BL21-CodonPlus -RIL series** of strains, which contain extra copies of the E. coli argU, ileY, and leuW tRNA genes. This modification allows for a high-level expression of proteins that are difficult to express in conventional E. coli hosts due to the codon bias of the gene of interest. The BL21-CodonPlus-RIL strains can eliminate the need to move the gene of interest into eukaryotic expression systems.

BL21-Gold(DE3) cells are the strain of choice for high-level production of heterologous proteins expressed from the T7-promoter. These strains have been engineered to contain an inducible T7-RNA polymerase. They are also EndA- to allow high-quality miniprep DNA preparation.1 BL21 competent cells are derivatives of E. coliB and lack the Lon protease and the ompT protease genes that may cause instability and degradation of the recombinant protein.

Table 1
Rarest Codons (from E. coli) Preferred by Selected Organisms

Codon frequencies are expressed as codons used per 1000 codons encountered. A complete compilation of codon usage of the sequences placed in the gene bank database can be found at http://www.dna.affrc.go.jp/~nakamura/codon.html Codon frequencies of more than 15 codons/1000 codons are shown in bold to help identify a codon bias that may cause problems for high-level expression in E. coli. The arginine codons AGG and AGA are recognized by the same tRNA (pr
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
2. New BL21-CodonPlus Cells Correct Codon Bias in GC-Rich Genomes
3. High-efficiency Derivatives of the BL21 Series for Protein Expression
4. A New C-Terminal GST Vector for Protein Production in S. pombe
5. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
6. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
7. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
8. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
9. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
10. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
11. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Codon Bias Adjusted Derivatives for Protein Expression

(Date:8/29/2014)... 2014 Three Lawrence Livermore researchers ... Hydrogen Production R&D Award for their research ... using sunlight. , Shared with collaborators from the ... of Nevada, Las Vegas (UNLV), the award recognizes ... photoelectrochemical solar-hydrogen production and corrosion processes. , These ...
(Date:8/29/2014)... Boston, MA, San Antonio, TX (PRWEB) August 29, ... audited, ISO 9001:2008 and ISO 13485:2003 certified, GAMP® ... it has been awarded an international Phase II ... patients with non-Hodgkin’s lymphoma. , Throughout this trial, ... services including, but not limited to, protocol and ...
(Date:8/28/2014)... Best Sanitizers, Inc., the first company to ... asks food processors and food handling professionals to prepare ... currently using to Best Sanitizers’ Alpet® E3 Plus ... fighting cross-contamination and the spread of pathogens in the ... key criteria that make a hand sanitizer most effective, ...
(Date:8/28/2014)... are important devices in spintronics, an electronic which ... electrons but also on their spin and the ... electric into magnetic signals and vice versa. Recently, ... the Institute of Physics at Johannes Gutenberg University ... Prague, and Japan, has for the first time ...
Breaking Biology Technology:Livermore Team Awarded for Hydrogen Production Research 2Intrinsic Imaging Awarded Phase II Clinical Trial to Assess New Treatment for Non-Hodgkin’s Lymphoma 2Food Processors and Food Handlers are Encouraged to Prepare for Fall Harvest by Evaluating Their Current Hand Sanitizer Using Nine Key Criteria 2Food Processors and Food Handlers are Encouraged to Prepare for Fall Harvest by Evaluating Their Current Hand Sanitizer Using Nine Key Criteria 3A new, tunable device for spintronics 2A new, tunable device for spintronics 3
... 10 Cambridge Research &,Instrumentation, Inc. (CRi) announces ... that converts any brightfield or,fluorescent microscope into a ... larger specimens, such as embryos,zebrafish, plants, tissue culture ... onto a fluorescent macroscope. The Nuance 2 system ...
... Faster, Easier,Quantification of Protein Biomarkers in Translational Medicine and Adaptive ... ... WALTHAM, Mass., Dec. 10 Decision Biomarkers Inc (DBI),a leading ... today announced the company,s,PATH(R) technology has been awarded United States ...
... International,(Nasdaq: PRXL ) has appointed Charles A. ... Policy and Strategic Reimbursement Services, a,unit based in ... team of,experts dedicated to helping PAREXEL,s clients provide ... and therapies they require. His,responsibilities include managing reimbursement ...
Cached Biology Technology:CRi Announces Nuance 2, the Next-Generation Multispectral Imaging System for Brightfield and Fluorescent-Based Microscopy 2Decision Biomarkers Awarded U.S. Patent for Protein Array Surface Chemistry 2PAREXEL Appoints Charles A. Stevens as Vice President/General Manager of Health Policy and Strategic Reimbursement and Strengthens Capabilities 2PAREXEL Appoints Charles A. Stevens as Vice President/General Manager of Health Policy and Strategic Reimbursement and Strengthens Capabilities 3PAREXEL Appoints Charles A. Stevens as Vice President/General Manager of Health Policy and Strategic Reimbursement and Strengthens Capabilities 4PAREXEL Appoints Charles A. Stevens as Vice President/General Manager of Health Policy and Strategic Reimbursement and Strengthens Capabilities 5
(Date:8/29/2014)... researcher from the University of Texas Medical Branch at Galveston ... monoclonal antibodies can completely protect monkeys against a lethal dose ... a time when the disease is severe. , Thomas Geisbert, ... Nature discussing advances in Ebola treatment research. The ... the most deadly of pathogens, with fatality rates of up ...
(Date:8/29/2014)... the type and number of connections in transcription factor ... robustness in a study published in PLOS Computational ... the assortativity signature contributes to a network,s resilience against ... the out-out assortativity of TFN models has a greater ... three types of assortativity," said Dov A. Pechenick, PhD, ...
(Date:8/29/2014)... is available in German . ... between organisms is considered the oldest form of communication. ... conspecifics, for example, the sexual attraction between males and ... and to coordinate reproductive behavior in males and females. ... of the Algarve in Faro, Portugal, and at the ...
Breaking Biology News(10 mins):Leading Ebola researcher at UTMB says there's an effective treatment for Ebola 2Assortativity signatures of transcription factor networks contribute to robustness 2Ready for mating at the right time 2Ready for mating at the right time 3
... animal compares males to choose a mate, she can't order ... to rely on external cues that may indicate genetic quality. ... the peacock's tail, as cues that females might use. , ... between male genetic quality and ornament quality can be maintained. ...
... progenitor cells differentiate into cartilage cells when one master ... College of Medicine researchers in a report that appears ... of Sciences. , Skeletons are made of bone and ... stem cell, said Dr. Brendan Lee, associate professor of ...
... into brain cells on a new three-dimensional scaffold of ... living body than any other cell culture system. , ... invention-which may one day replace the ubiquitous Petri dish ... PLoS ONE. Shuguang Zhang, associate director of MIT's Center ...
Cached Biology News:Female pronghorns choose mate based on substance as well as show 2Signaling for cartilage 2MIT creates 3-D scaffold for growing stem cells 2MIT creates 3-D scaffold for growing stem cells 3
... Labsystems Wellmix is a high-quality four-place microplate ... The shaking speed is adjustable from 100 ... speed and a small orbit ensure effective ... time is quick and easy to set ...
Mouse monoclonal [AZ24] to Polo like kinase ( Abpromise for all tested applications)....
... The dotLab System brings together ... diffraction and immobilized capture surfaces. This ... simple technique for the detection of ... of fluorescent labels. , Protein-specific capture ...
... LabWorks Software provides a comprehensive experiment ... use drop down menus yet sophisticated and ... with dozens of CCD cameras for easy ... , Automated Digital Video Playback ...
Biology Products: