Navigation Links
Codon Bias-Adjusted BL21 Derivatives for Protein Expression


High-level expression of difficult heterologous proteins

Carsten-Peter Carstens Anna Waesche
Stratagene

Stratagene has created variants of the Epicurian Coli BL21-Gold cells * called the BL21-CodonPlus -RIL series** of strains, which contain extra copies of the E. coli argU, ileY, and leuW tRNA genes. This modification allows for a high-level expression of proteins that are difficult to express in conventional E. coli hosts due to the codon bias of the gene of interest. The BL21-CodonPlus-RIL strains can eliminate the need to move the gene of interest into eukaryotic expression systems.

BL21-Gold(DE3) cells are the strain of choice for high-level production of heterologous proteins expressed from the T7-promoter. These strains have been engineered to contain an inducible T7-RNA polymerase. They are also EndA- to allow high-quality miniprep DNA preparation.1 BL21 competent cells are derivatives of E. coliB and lack the Lon protease and the ompT protease genes that may cause instability and degradation of the recombinant protein.

Table 1
Rarest Codons (from E. coli) Preferred by Selected Organisms

Codon frequencies are expressed as codons used per 1000 codons encountered. A complete compilation of codon usage of the sequences placed in the gene bank database can be found at http://www.dna.affrc.go.jp/~nakamura/codon.html Codon frequencies of more than 15 codons/1000 codons are shown in bold to help identify a codon bias that may cause problems for high-level expression in E. coli. The arginine codons AGG and AGA are recognized by the same tRNA (pr
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
2. New BL21-CodonPlus Cells Correct Codon Bias in GC-Rich Genomes
3. High-efficiency Derivatives of the BL21 Series for Protein Expression
4. A New C-Terminal GST Vector for Protein Production in S. pombe
5. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
6. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
7. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
8. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
9. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
10. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
11. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Codon Bias Adjusted Derivatives for Protein Expression

(Date:5/16/2008)...ulti-center registry presented at conference, NAT...n Scientific,Corporation (NYSE: BSX ) today annou...ter patient registry documenting the safety and cl...tion System will be presented at,Digestive Disease...iego,CA. Registry results will be announced in th...
(Date:5/16/2008)...Company,s partner Aqua Bounty to scale back ...gram - TSX symbol: SBS,...ys Genetics Inc.,(TSX:SBS), a biotechnology compan...or metabolic and cardiovascular diseases, today an...ImmunoSphere(TM) shrimp feed additive,program, Aqu...
(Date:5/16/2008)...6 /- Highlighting the,compa...rrent product,pipeline, Michael V. Novinski, Presi...aq: EMIS ), was featured in an extensive intervie... (TWST). Established more than 40,years ago, TWST ...ding,CEOs, analysts and money managers, and publis...
(Date:5/16/2008)...wire/ -- Alba Therapeutics Corporation,announced t... patient in a,twelve-week Phase IIb trial with ora...arrier dysfunction, for the treatment of patients ...center, randomized, double-blind,placebo-controll...al,efficacy, safety and tolerability of larazotide...
Breaking Biology Technology:Boston Scientific's SpyGlass(R) Direct Visualization System to Be Prominently Featured at Digestive Disease Week(R) 2Boston Scientific's SpyGlass(R) Direct Visualization System to Be Prominently Featured at Digestive Disease Week(R) 3Boston Scientific's SpyGlass(R) Direct Visualization System to Be Prominently Featured at Digestive Disease Week(R) 4Boston Scientific's SpyGlass(R) Direct Visualization System to Be Prominently Featured at Digestive Disease Week(R) 5SemBioSys provides update on shrimp feed additive, Immunosphere(TM) 2SemBioSys provides update on shrimp feed additive, Immunosphere(TM) 3Michael V. Novinski, Emisphere President/CEO Featured in the Wall Street Transcript 2Michael V. Novinski, Emisphere President/CEO Featured in the Wall Street Transcript 3Alba Therapeutics Announces the Enrollment and Randomization of the First Patient in the Phase IIb Clinical Trial for Larazotide Acetate (AT-1001) for the Treatment of Active Celiac Disease 2Alba Therapeutics Announces the Enrollment and Randomization of the First Patient in the Phase IIb Clinical Trial for Larazotide Acetate (AT-1001) for the Treatment of Active Celiac Disease 3
...on Cassettes , siRNA expres...pportunities to study gene silencing. Unfortunatel...ectors is labor and time intensive. , ...ssion , Cassette Kits provide a... siRNA expression cassettes (SECs) that ca...
...ption Kit For One Step qRT-PCR , ... II and other RNase H MMLV reverse transcri...argets , over a 106-fold range , ... as 500 fg total RNA 1/20th of a single cell! ... complete in 15 min , ,...
...d cell sorting (FACS) is a , tool for mea...rties of , individual cells using fluores..., associated with the cell. This technology... given population down to the level of a single ce...ation from 1-1000 Cells. , Dr. Daniel Dou...
...malian RNA from , complex mixtures of...procedure takes less than 2 hours , ... Seamless integration with , MICR..., Enables microarray expression an...from host cells , Ambion,s new ,...
Other Biology Technology:GENE SILENCING WITH PCR PRODUCTS 2GENE SILENCING WITH PCR PRODUCTS 3GENE SILENCING WITH PCR PRODUCTS 4GENE SILENCING WITH PCR PRODUCTS 5GENE SILENCING WITH PCR PRODUCTS 6GENE SILENCING WITH PCR PRODUCTS 7GENE SILENCING WITH PCR PRODUCTS 8HIGH SENSITIVITY qRT-PCR 2HIGH SENSITIVITY qRT-PCR 3HIGH SENSITIVITY qRT-PCR 4HIGH SENSITIVITY qRT-PCR 5Cells-to-cDNA II Using FACS Sorted Cells 2Bacterial RNA Isolation From Infected Eukaryotic Hosts 2Bacterial RNA Isolation From Infected Eukaryotic Hosts 3Bacterial RNA Isolation From Infected Eukaryotic Hosts 4Bacterial RNA Isolation From Infected Eukaryotic Hosts 5Bacterial RNA Isolation From Infected Eukaryotic Hosts 6
(Date:5/15/2008)...he human race was divided into two separate groups...e, says a Tel Aviv University mathematician. Clima...itions may have caused and maintained the separati...thematical Sciences at Tel Aviv University, worked...cal Center to analyze African DNA. Their goal was ...
(Date:5/15/2008)..., Ill. (May 15, 2008) Argonne National Laboratory...th Savannah River National Laboratory (SRNL) to co...gement research projects in support of critical U....nt national priorities. , "The MOU allows Argonn...Argonne director Robert Rosner said. "Argonne has ...
(Date:5/15/2008)... Institute researchers Karen Staehling-Hampton, Ph...livier Pourqui, Ph.D., Investigator, collaborated ... genes known to cause spinal mutations in chick an...in human patients with congenital vertebral abnorm... the Web site of the American Journal of Human Gen...
(Date:5/15/2008)... business practice usually reserved for boosting p...ucing their energy use? Writing in the Internation...(IJSSCA), researchers in India provide an answer. ...shik of NC College of Engineering in Haryana, Indi...t the forefront of company efforts. In a global ec...
Breaking Biology News(10 mins):Long lost sisters 2Argonne-SRNL agreement supports critical DOE, national priorities 2Stowers Institute researchers identify gene linked to vertebral defects in patient populations 2Adding up business and energy 2CardioDynamics Ranked in Deloittes Technology Fast 50 Program for San Diego 1284 1CardioDynamics Ranked in Deloittes Technology Fast 50 Program for San Diego 1284 2CardioDynamics Ranked in Deloittes Technology Fast 50 Program for San Diego 1284 3CardioDynamics Ranked in Deloittes Technology Fast 50 Program for San Diego 1284 4Data from Three Clinical Trials for Perforomist 28TM 29 Inhalation Solution to be Presented at CHEST 2007 on October 24 2007 531 1Data from Three Clinical Trials for Perforomist 28TM 29 Inhalation Solution to be Presented at CHEST 2007 on October 24 2007 531 2Data from Three Clinical Trials for Perforomist 28TM 29 Inhalation Solution to be Presented at CHEST 2007 on October 24 2007 531 3Data from Three Clinical Trials for Perforomist 28TM 29 Inhalation Solution to be Presented at CHEST 2007 on October 24 2007 531 4Data from Three Clinical Trials for Perforomist 28TM 29 Inhalation Solution to be Presented at CHEST 2007 on October 24 2007 531 5IASP declares the Global Year Against Pain in Women 3775 1IASP declares the Global Year Against Pain in Women 3775 2IASP declares the Global Year Against Pain in Women 3775 3UD named a regional research participant in National Children 26 23146 3Bs Study 819 1UD named a regional research participant in National Children 26 23146 3Bs Study 819 2
...sue throughout the body have an undesirable side e.... To overcome this hurdle, Johns Hopkins researche...hemical that helps them slip through this sticky b...particles, the researchers also discovered that mu...thought, providing a doorway that should allow lar...
...idney tumors through percutaneous cryoablation sho...s for surgery. Their early findings showing short...patients are published in this month’s issue of Ra...s is surgery, providing a high likelihood of a lon... option, and Mayo’s urologists and radiologists co...
...tory of all 4,500 mammals on Earth shows that they... dinosaurs, says new research published in Nature ...entists at Imperial College London and the Zoologi...reviously accepted theory that the Mass Extinction...on years ago prompted the rapid rise of the mammal...
...so realistic that the virtual forests of diverse m...ation patterns that precisely mimic their real-wor...first to reflect the vast diversity of the invisib...d green plants that dominate the ocean and produce...so in a way that is consistent with the way real-w...
Other Biology News:Coated nanoparticles solve sticky drug-delivery problem 2Coated nanoparticles solve sticky drug-delivery problem 3Cryoablation -- A new treatment option for some kidney tumor patients 2Cryoablation -- A new treatment option for some kidney tumor patients 3Dinosaur extinction didn't cause the rise of present-day mammals, claim researchers 2MIT's ocean model precisely mimics microbes' life cycles 2MIT's ocean model precisely mimics microbes' life cycles 3
Mouse polyclonal antibody raised against a partial recombinant XAGE2. Immunogen: XAGE2 (NP_570133, 44 a.a. ~ 112 a.a) partial recombinant protein with GST tag. Accession Number: NM_130777
...urate Results Laser Capture Microdissection (LCM)...ity and accuracy of molecular assays by starting w...llular structures isolated from whole tissue or cy...ction-developed in 1996 at the National Institutes...
Mouse polyclonal antibody raised against a partial recombinant ZFHX4. Immunogen: ZFHX4 (NP_078997, 2 a.a. ~ 94 a.a) partial recombinant protein with GST tag. Accession Number: NM_024721 Pr
EPA II Buffer is a diluent formulated to stabilize the activity of Chemicon horseradish peroxidase (HRP)-antibody conjugates at high conjugate dilution for extended periods.
Biology Products: