Navigation Links
Coated Plate Immunoassay on the MicroBeta - eg. TSH - IRMA

0.9% NaCl add water 90 ml

2. Add anti-TSH into coating buffer (antibody concentration 1.0 μg/200μL/well)

3. Dispense 200 μL/well

4. Incubate overnight in a moist chamber

5. Wash 3 x with the wash solution

6. Saturate 1 hour at room temperature with 200 μL of 50 mM TSA-buffer containing 0.5% BSA
Saturation Buffer formulation:
50 mM Tris = 0.606 g Tris (Sigma)
0.9% NaCl = 0.9 g
conc HCl 0.30 mL
7.5% BSA 6.66 mL
add 100 mL, pH 7.77

7. Aspirate to dryness

8. Cover with re-sealable seal and store at 4C

**ASSAY PROCEDURE OF TSH

Previously coated plates. Farmos 125I approx. 450 kBq/11 ml anti-TSH.

ASSAY PROCEDURE

1. Add 100 μL standard and samples in duplicates to the plates

2. Add 100 μL tracer

3. Incubate at room temperature for 2 hours (or overnight)

4. Wash 5 x with wash solution with a suitable plate washer

Wash solution formulation:
Tris-HCl 0.005 M (5 mM), pH 7.5
0.9% NaCl
0.005% Tween-20

5. Decant excess wash solution (& dry if using Meltilex)

6. Add scintillant to each well, shake for 5 minutes on a plate shaker

7. Count in MicroBeta


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. Isolation of Low Molecular Weight Digestion Products of the Human Platelet Thromboxane A2 Receptor, Rev A
3. Counting FlashPlates on the Wallac MicroBeta
4. Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor
5. Use of Novel FlashPlate Technology to Measure cAMP Accumulation in Chinese Hamster Ovary Cells Expressing Human -2 Adrenoreceptors
6. Use of FlashPlate Technology for In Vitro Measurement of 125I-Labeled TGF-1 Binding on Chimeric Extracellular Domain of Type II Transforming Growth Factor Receptor
7. In Vitro Measurement of the Second Messenger cAMP: RIA vs. FlashPlate
8. Direct Counting of Millipore MultiScreen Filtration Plates.
9. Evaluation of FlashPlate in a Helicase Assay
10. 96 Well Pipetting Precision and Accuracy Using the RapidPlate 96/384 Workstation
11. Sciclone ALH 3000 Liquid Handler: Functional Characterization of the High Volume Head - Part 1: Plate-to-Plate Precision Testing
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Coated Plate Immunoassay the MicroBeta TSH IRMA

(Date:9/19/2014)... 2014  An entire month is dedicated to one ... Charcot-Marie-Tooth disease (CMT) – a debilitating neuromuscular disease affecting ... atrophy, weakness, and foot and hand deformities. CMT affects ... CMT is a progressive disease, and over time patients ... devices such as leg braces, wheelchairs and scooters to ...
(Date:9/19/2014)... , September 19, 2014 BioTech ... developments, patents and results of studies and trials.  Companies ... and Company (NYSE: LLY ), Gilead Sciences ... CELG ) and Arena Pharmaceuticals Inc. (NASDAQ: ... in developing prodrug therapeutics for the treatment of cancer, ...
(Date:9/19/2014)... 2014 , The first grants ... Paediatric Endocrinology (ESPE) Meeting  Investment supports innovative ... in the field of growth  , EMD ... Germany , today announced the first recipients of ... awards were announced during a Satellite Symposium organized by ...
(Date:9/18/2014)... , Sept. 18, 2014 About ... testing, is performed outside a clinical laboratory. It ... in administrating appropriate treatments and medication. POCT is ... prevalence of lifestyle diseases such as diabetes, heart ... user-friendly. POCT devices are standardized to minimize errors ...
Breaking Biology Technology:September is Charcot-Marie-Tooth (CMT) Awareness Month - Scheduled events, activities and important partnerships announced throughout the month! 2Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 2Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 3Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 4Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 5Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 6Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 7EMD Serono Awards Grant for Growth Innovation (GGI) for the First Time 2EMD Serono Awards Grant for Growth Innovation (GGI) for the First Time 3POCT Market in China 2014-2018 2
... MA , July 21 /PRNewswire-FirstCall/ - SemBioSys Genetics ... proteins and oils utilizing its novel plant expression systems, today ... as President of US and International Operations. Mr. Pierce will ... will offer the Company a broader market to better exploit ...
... July 20 Caliper Life Sciences, Inc. (Nasdaq: CALP ... financial results at 9:00 a.m. ET on Tuesday, August ... McAree , Chief Financial Officer, will host the call. Management will discuss ... , , , ...
... EXTON, Pa. , July 20 Fibrocell Science, Inc. ... autologous cell therapies for aesthetic, medical and scientific applications, announced today that ... its board of directors. , , ... Dr. Korkos currently serves as President of both Plastic Surgery Associates and ...
Cached Biology Technology:SemBioSys Appoints President of US and International Operations and Announces the Appointments of Three Vice Presidents 2SemBioSys Appoints President of US and International Operations and Announces the Appointments of Three Vice Presidents 3SemBioSys Appoints President of US and International Operations and Announces the Appointments of Three Vice Presidents 4Caliper Life Sciences' Second Quarter 2010 Financial Results Conference Call Notice 2Fibrocell Science, Inc. Announces Addition of Dr. George J. Korkos to Board of Directors 2Fibrocell Science, Inc. Announces Addition of Dr. George J. Korkos to Board of Directors 3
(Date:9/18/2014)... stressed they are often grouchy, grumpy, nasty, distracted or ... EPFL have just highlighted a fundamental synaptic mechanism that ... of social skills and cognitive impairment. When triggered by ... the brain. This was revealed by a work published ... team went to look for answers in a region ...
(Date:9/18/2014)... research has found clownfish larvae can swim up to 400 ... able to cope with environmental change. , Clownfish spend their ... but as babies they must wander the open ocean, says ... Excellence for Coral Reef Studies (Coral CoE) at James Cook ... go, but now we,ve been given a rare glimpse into ...
(Date:9/17/2014)... creation of the Santa Ana Wildfire Threat Index ... of the powerful, hot, dry Santa Ana wind, which ... was introduced Sept. 17 by the U.S. Forest Service, ... Electric. , The index includes four classification levels ranging ... help fire agencies and other emergency responders, the media ...
Breaking Biology News(10 mins):How stress tears us apart 2Nemo's epic journey to find a new home 2UCLA scientists play key role in developing new Santa Ana Wildfire Threat Index 2
... Norwich scientists are on the trail of some of the ... targets are the parasitic water fungus that causes powdery mildew ... and tomatoes and downy mildew in cruciferous vegetables and other ... for a while," said Professor Sophien Kamoun, head of the ...
... original sweetener, manufactured by honey bees long before humans ... gatherers, as do ancient Egyptian reliefs. From Mesopotamia to ... every human culture and cuisine. "Honey is fascinating; ... unbelievable activity. It,s just an amazing substance," said University ...
... how a network of repair proteins enables bacteria to prioritise ... DNA molecules that carry the instructions necessary for living cells ... the University of Bristol and published in Molecular Cell ... the DNA repair systems of bacteria and humans than had ...
Cached Biology News:Gene hunters tackle crop diseases 22 books explore the history and delights of honey, bees and beehives 22 books explore the history and delights of honey, bees and beehives 32 books explore the history and delights of honey, bees and beehives 4New research reveals details of microbe's extraordinary maintenance and repair system 2
...
... deuterium atoms at the 9, 10, 12, and ... an internal standard for the quantification of 13(S)-HODE ... by incubation of linoleic acid with plant and ... the adhesion of tumor cells to the endothelium ...
... Cells and TriEx Insect Cell Medium are ... constructs. This cell/medium combination has been selected ... high protein expression levels observed specifically with ... note that our Ready-Plaque Sf9 Cells and ...
...
Biology Products: