Navigation Links
Coated Plate Immunoassay on the MicroBeta - eg. TSH - IRMA

COATED PLATE ASSAYS

The MicroBeta is and ideal instrument for the counting of radioimmunoassays based on coated microtitration plates. The following method describes a typical assay protocol for a TSH assay. The data presented compare the standard immunoassay microtitration plates with those manufactured by PerkinElmer Life Sciences.

The basic assay procedure is straightforward:

(1) Coat the wells according to the standard method described* or use commercially coated plates.

(2) Carry out the immunoassay procedure as described** - eg react the antigen to the coated antibody.

(3) Add Optiphase HiSafe II into each well and place on a plateshaker for 5 minutes (or if the plate is dried, add Meltilex using the Meltipipette 1495-034/035, and heat in oven until liquified. Allow to reset and count).

(4) Place into the MicroBeta cassette (1450- 105) and count in the MicroBeta (remember to correct for crosstalk prior to counting the sample plates).

(5) Collect and analyze the data with MultiCalc.

As a comparison two other commercially available plates (Labsystems-Polysorp and Nunc-Maxisorp) were also assayed in parallel to the Wallac (1450-401) plate to show any observable differences in plate performance.

RESULTS

The graphs (Fig.1) clearly illustrate that the MicroBeta can be used in clinical chemistry laboratories, the sensitivity required for this assay can be obtained by the MicroBeta. Additionally there appears to be little difference between using the Wallac plate to using specially surface treated plates from other companies.

*COATING PROCEDURE

1. Coating Buffer formulation:
50 mM carbonate buffer, pH 9.6
22.5 mM Na2CO3
27.5 mM NaHCO3

'"/>

Source:


Page: All 1 2

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. Isolation of Low Molecular Weight Digestion Products of the Human Platelet Thromboxane A2 Receptor, Rev A
3. Counting FlashPlates on the Wallac MicroBeta
4. Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor
5. Use of Novel FlashPlate Technology to Measure cAMP Accumulation in Chinese Hamster Ovary Cells Expressing Human -2 Adrenoreceptors
6. Use of FlashPlate Technology for In Vitro Measurement of 125I-Labeled TGF-1 Binding on Chimeric Extracellular Domain of Type II Transforming Growth Factor Receptor
7. In Vitro Measurement of the Second Messenger cAMP: RIA vs. FlashPlate
8. Direct Counting of Millipore MultiScreen Filtration Plates.
9. Evaluation of FlashPlate in a Helicase Assay
10. 96 Well Pipetting Precision and Accuracy Using the RapidPlate 96/384 Workstation
11. Sciclone ALH 3000 Liquid Handler: Functional Characterization of the High Volume Head - Part 1: Plate-to-Plate Precision Testing
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Coated Plate Immunoassay the MicroBeta TSH IRMA

(Date:5/1/2015)... BETHESDA, Md. , May 1, 2015  Northwest ... biotechnology company developing DCVax® personalized immune therapies for solid ... on the $28.5 million second tranche of a $40 ... ("Woodford"). Woodford first invested $25 million in ... 2015, Woodford entered into an agreement with the Company ...
(Date:5/1/2015)... /CNW/ - Softchoice, a leading North American IT solutions and managed ... of two Cisco® Partner Summit Country awards. ... Security Partner of the Year for Canada ... in Montreal . "We ... awards at this year,s conference," said David MacDonald , ...
(Date:5/1/2015)... 1, 2015 According to ... Product (Equipment, Consumables), by Type (Monoclonal, Polyclonal), by ... Research Institutes) - Analysis & Global Forecast to ... global Antibody Production Market for the forecast period ... to reach $2.572 Billion by 2019 from $1.425 ...
(Date:4/30/2015)... DIEGO , April 30, 2015   Tamir ... announced today an update on its Ebola antiviral therapy ... Tamir,s leading candidates, TMR004, is currently being evaluated and ... of the battle against Ebola. During the first quarter ... And Leadership Meeting To Accelerate The Evaluation of Potential ...
Breaking Biology Technology:NW Bio Closes On $28.5 Million Second Tranche Of Woodford $40 Million Financing 2NW Bio Closes On $28.5 Million Second Tranche Of Woodford $40 Million Financing 3Softchoice recognized with two Country Awards at Cisco Partner Summit 2015 2Antibody Production Market worth $2.572 Billion by 2019 2Antibody Production Market worth $2.572 Billion by 2019 3Antibody Production Market worth $2.572 Billion by 2019 4Tamir Biotechnology Provides Update on TMR004 Development Against Mutating Ebola 2Tamir Biotechnology Provides Update on TMR004 Development Against Mutating Ebola 3Tamir Biotechnology Provides Update on TMR004 Development Against Mutating Ebola 4
... Better Option for Humans, EGG HARBOR TOWNSHIP, ... first time, The British Institute of Homeopathy, U.S.A. ... U.S. medical and,homeopathic practitioners for a low-introductory cost ... therapy is more advantageous because,it does not carry ...
... Inc. (Nasdaq and SWX: BMRN) announced today the ... Research. Former Vice President of,Research and Development for ... biotechnology patents and the recipient of a,collection of ... Year Awards from Intellectual Property Owners, Inc., ...
... (Nasdaq: KNDL ), a,leading, global full-service clinical ... Officer Candace Kendle, PharmD,will participate in the Wachovia ... the Langham Hotel in Boston, Jan. 29-31, 2008. ... 1:30 p.m. Eastern Time., (Logo: http://www.newscom.com/cgi-bin/prnh/20030429/KNDLLOGO ...
Cached Biology Technology:BioMarin Appoints Dr. Gordon Vehar, Vice President of Research 2BioMarin Appoints Dr. Gordon Vehar, Vice President of Research 3Kendle to Present at the Wachovia 2008 Healthcare Conference 2
(Date:3/31/2015)... , March 31, 2015   Guidepoint , ... launch of the Post-Surgical Pain Management TRACKER, part of ... tracking usage trends in the medical device and therapeutics ... to data including treatment volumes, market share, and adoption ... pain associated with joint surgery. The Post-Surgical ...
(Date:3/24/2015)... Research and Markets ( http://www.researchandmarkets.com/research/kvdtcw/face_and_voice ) has announced ... Strategic Business Report" report to their offering. This ... US$ Thousands by the following Segments: Face Biometrics, and Voice Biometrics. ... Canada , Japan , Europe ... & Africa , and ...
(Date:3/23/2015)... , March 23, 2015   HOYOS Labs ... management company, today announced that the Company will demonstrate ... both enterprise and consumers at Connect:ID on March 23 ... HOYOS Labs will highlight the IEEE Biometric Open ... ; and enterprise access control system. BOPS was ...
Breaking Biology News(10 mins):Guidepoint Launches Post-Surgical Pain Management TRACKER 2Guidepoint Launches Post-Surgical Pain Management TRACKER 3Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 2Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 3Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 4Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 5Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 6HOYOS Labs to Showcase Host of Biometrics Technologies at Connect:ID in Washington, D.C. 2HOYOS Labs to Showcase Host of Biometrics Technologies at Connect:ID in Washington, D.C. 3
... found at Dinosaur Cove in Victoria, Australia, were likely ... the oldest known bird tracks in Australia. The ... the footprints led by Anthony Martin, a paleontologist at ... which include tracks, burrows and nests. The study was ...
... sandpipers ( Calidris pusilla ) feeding during low tide ... Beauchamp, an ornithologist and research officer at the University ... the periphery remained alert and used short pecks to ... the group relaxed their vigilance and fed on a ...
... This news release is available in French ... activity during a cognitive task even in some people who are ... University of Montreal and Boston,s Brigham and Women,s Hospital. The findings ... how quickly light impacts on cognition. "We were stunned to discover ...
Cached Biology News:Tell-tale toes point to oldest-known fossil bird tracks from Australia 2Tell-tale toes point to oldest-known fossil bird tracks from Australia 3Bird buffet requires surveillance 2Study with totally blind people shows how light helps activate the brain 2
... PCR System is the only PCR system ... and every time. It provides accuracy, consistency, ... source or the property of the DNA ... Enzyme Mix, a unique blend of thermostable ...
ID clarifier: With ethidium bromide (50 μg/ml)...
Extremely high efficiency packaging extracts useful for transfecting our EpiFOS™ or CopyControl™ Fosmids, or any DNA vector with cos sites. A tremendous value when conpared to the competition...
... Buffer is a convenient, premixed, ... samples and Perfect RNA Markers. ... no dilution and is suitable ... Simply mix equal volumes of ...
Biology Products: