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Clostridium botulinum

Homogenize by gently mixing with pipette several times. Transfer mixture into a prechilled cuvette. Keep on ice for 2 min.
  • Wipe moisture from the cuvette and insert the cuvette into the device.
  • Electroporation:

    Mode Prokaryotes O Voltage (V) 2,500 V Time constant (T) 5 ms
  • Dilute cells into 10 ml TPGY medium with 25 mM MgCl2 and incubate 5 hours at 34 C.
  • Plate cells on selective TPGY agar plates; incubate at 34 C for 2-3 days.
    • Expected Results: Transformation efficiency up to 3 x 103 transformants/g of DNA.


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