Navigation Links
Clostridium botulinum

Multiporator / Electroporator 2510 Transformation Protocol Protocol No. 4308 915.509 12/2001 Microorganism Clostridium botulinum Cell type Bacteria, gram positive Molecules injected Plasmid DNA (pGK12 in water) Growth medium TPGY medium Washing solution 10% PEG 8000 Electroporation solution 10% PEG 8000 Outgrowth medium TPGY medium with 25 mM MgCl2 Cuvette 4 mm gap width Reference Zhou Y. and Johnson E. A. 1993 Biotechnology Letters 15, No. 2 121-126 Making electrocompetent cells:

1. Grow cells in TPGY medium at 37 C to an O.D.660 of 0.8. 2. Harvest by centrifugation. Wash in 0.8 volumes of PEG at 4 C, keep cells cold during the entire procedure. 3. Resuspend cells in 0.8 ml 10% PEG (number of cells: 4 x 108 cells/ml), keep on ice.

Electroporation of cells:

  1. Add 1 g plasmid DNA to 800 l of electrocompetent cells.
    '"/>


Source:


Page: All 1 2

Related biology technology :

1. Clostridium botulinum
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Clostridium botulinum

(Date:1/14/2014)... and BETHESDA, Md. , ... joining together with two institutes from the National Institutes ... tools for bringing safer, more effective treatments to patients ... National Center for Advancing Translational Sciences (NCATS) and the ...
(Date:1/14/2014)... , Jan. 14, 2014  RXi Pharmaceuticals Corporation (OTCQX: ... and commercializing innovative therapies addressing major unmet medical ... received the Notice of Allowance from the United ... self-delivering RNAi compounds (sd-rxRNA®), for the treatment of ...
(Date:1/14/2014)... USA, and Cardiff, UK (PRWEB) January 13, 2014 ... technology development leader with more than 20 years in ... for optics and photonics . Hainsey will serve as ... delighted to have Dr. Hainsey join SPIE as our ...
(Date:1/14/2014)... (PRWEB) January 14, 2014 Independent publisher ... California, and Montreal, Canada, has big ideas for 2014 ... The press's goals are to produce high-quality, low-cost, DRM-free ... support local businesses. , The first major development ...
Breaking Biology Technology:Organovo Announces Collaboration with National Institutes of Health 2Organovo Announces Collaboration with National Institutes of Health 3RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 2RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 3Photonics R&D Leader Bob Hainsey Joins SPIE Technical Staff 2Bitingduck Press Looks Forward to 2014 With Multimedia E-books 2
... Report the Use of Haptoglobin Typing,to Predict Cardiovascular ... MONTVALE, N.J., Nov. 10 Synvista,Therapeutics, Inc. ... Annual Scientific,Sessions of the American Heart Association, underway ... determine haptoglobin (Hp) genotype utilizing,intellectual property licensed from ...
... Nov. 10 SenesTech, Inc. has launched,the regulatory ... the first,ContraPest(R) product with the government of Australia. ... rice field rat species, registration,of ContraPest(R) bait will ... management product to the agricultural ministries of Southeast,Asia. ...
... with more than $500 million in ... cash - Conference ... ... ISIS ) today announced its financial results,for the third quarter ended September 30, 2008. Isis, ...
Cached Biology Technology:Synvista Therapeutics Announces Availability of Haptoglobin Genotyping Technology at The Annual Scientific Sessions of the American Heart Association 2Synvista Therapeutics Announces Availability of Haptoglobin Genotyping Technology at The Annual Scientific Sessions of the American Heart Association 3Synvista Therapeutics Announces Availability of Haptoglobin Genotyping Technology at The Annual Scientific Sessions of the American Heart Association 4Synvista Therapeutics Announces Availability of Haptoglobin Genotyping Technology at The Annual Scientific Sessions of the American Heart Association 5SenesTech, Inc. Commences Product Registration Process With Australian Government 2Isis Reports Financial Results and Highlights for Third Quarter of 2008 2Isis Reports Financial Results and Highlights for Third Quarter of 2008 3Isis Reports Financial Results and Highlights for Third Quarter of 2008 4Isis Reports Financial Results and Highlights for Third Quarter of 2008 5Isis Reports Financial Results and Highlights for Third Quarter of 2008 6Isis Reports Financial Results and Highlights for Third Quarter of 2008 7Isis Reports Financial Results and Highlights for Third Quarter of 2008 8Isis Reports Financial Results and Highlights for Third Quarter of 2008 9Isis Reports Financial Results and Highlights for Third Quarter of 2008 10Isis Reports Financial Results and Highlights for Third Quarter of 2008 11Isis Reports Financial Results and Highlights for Third Quarter of 2008 12Isis Reports Financial Results and Highlights for Third Quarter of 2008 13Isis Reports Financial Results and Highlights for Third Quarter of 2008 14Isis Reports Financial Results and Highlights for Third Quarter of 2008 15
(Date:4/17/2014)... Current Biology on April 17 have discovered ... Brazilian insects, which represent four distinct but related species ... example of an animal with sex-reversed genitalia. , ... animals, Neotrogla is the only example in ... Yoshizawa from Hokkaido University in Japan. , During copulation, ...
(Date:4/17/2014)... is the process whereby the genetic information of ... as proteins, which have numerous different functions in ... important intermediary during gene expression, by relating the ... involved in manufacturing proteins. , By examining ... present in an organism at a given time, ...
(Date:4/17/2014)... Pine forests are chock full of wild animals and ... populations of fungi are churning away in the soil, ... , Despite the vital role these fungi play in ... A Stanford-led team of scientists has generated a genetic ... North America. The work was published this week in ...
Breaking Biology News(10 mins):In sex-reversed cave insects, females have the penises 2Rapid and accurate mRNA detection in plant tissues 2Stanford biologists help solve fungal mysteries 2Stanford biologists help solve fungal mysteries 3
... Southwestern Medical Center challenge one of the established views ... Every time we move, feel emotions, think or remember, ... messages to one another via chemical signals called neurotransmitters. ... sequester neurotransmitters and release them when needed into the ...
... all in the packaging. How nature wraps and tags genes ... from Harvard and the Massachusetts Institute of Technology (M.I.T.). They ... protein structure that surrounds the human genome. , Their findings ... switched on during development of the human body and in ...
... older are as,inevitable and obvious as “going gray,?yet ... of this usually unwelcome transformation.,In a report posted ... Science,researchers from Dana-Farber Cancer Institute and Children’s Hospital,Boston ... graying hair while,investigating the origins of malignant melanoma, ...
Cached Biology News:New research questions basic tenet of neuron function 2Protein Packages Found To Activate Genes; May Be What Regulates Development And Disease 2Protein Packages Found To Activate Genes; May Be What Regulates Development And Disease 3Weizmann Institute scientists develop a new approach for directing treatment to metastasized prostate cancer in the bones. 2Weizmann Institute scientists develop a new approach for directing treatment to metastasized prostate cancer in the bones. 3
... RC DC protein assay reagents package is used ... reducing agents and detergents. The RC DC protein ... reagents sufficient for 500 standard assays: 250 ml ... 250 ml alkaline copper tartrate solution, 2 L ...
... ProteoExtract™ Protein Precipitation Kit is designed ... proteins from aqueous samples. Proteomics sample ... presence of non-protein impurities such as ... protein sample that interfere with electrophoretic ...
... Pierce offers maleimide activated carrier proteins ... conjugates. Imject Maleimide Activated Carrier Proteins ... been preactivated with a heterobifunctional cross-linker ... stable, sulfhydryl-reactive carrier protein. Thus ...
... This kit allows for rapid, convenient, and ... simple bind/wash/elute process utilizes a spin column ... beads for concentration of proteins which were ... are strong ion exchangers, offering high binding ...
Biology Products: