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CleanSEQ on the ABI 3100

lary function. Samples loaded in formamide or in water overlaid with mineral oil had identical patterns of Phred20 read lengths over time (Figure 4). Thus, mineral oil had no detrimental effects on capillary performance.


Conclusions
We recommend eluting samples from CleanSEQ beads with 50l of water followed by application of 20l of mineral oil for use with ABI 3100 sequencers.
CleanSEQ samples eluted in water and overlaid with oil show no sample degradation after 6 days at room temperature, providing injection flexibility and eliminating the appearance of dye blobs.
Increased signal strengths using CleanSEQ and water elution allow increased dilution of BigDye Terminators resulting in substantial cost savings.
Injection of samples containing mineral oil does not adversely effect either short or long-term capillary performance.


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