Navigation Links
CleanSEQ on the ABI 3100


Removal of unincorporated dye-terminators is required prior to sequence delineation on capillary electrophoresis instrumentation. CleanSEQ has proven an efficient and highly effective dye-terminator removal system with the ABI 3700 and 3730xl sequencers. Samples run on the ABI 3100 are more prone to evaporation and exposure to air before machine injection which can have deleterious effects on sample integrity including the appearance of dye blobs. This application note will demonstrate the effective use of CleanSEQ for dye-terminator removal in combination with the ABI 3100.


Introduction
CleanSEQ has proven to outperform many other dye-terminator removal technologies when used in conjunction with the high throughput ABI PRISM 3700 and 3730xl sequencers. The ABI 3100 is a lower throughput instrument that unlike the 3700 and 3730xl systems, has historically given more reproducible results with formamide elution and injection. Formamide acts to reduce sample evaporation which helps maintain sample integrity. Formamide, however, is well known to significantly reduce signal strengths.

CleanSEQ is a magnetic bead dye-terminator removal system based on SPRI chemistry. Experimentation during CleanSEQ development indicated that samples eluted in water were of high quality and produced sequences with long Phred20 read lengths and much higher signal intensities than samples eluted in formamide. Our standard CleanSEQ protocol, therefore, recommends the elution of sequencing products in water (Figure 1). We believe the septa seals on the 3100 may not effectively prevent exposure to air, potentially s
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
2. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
3. Protocol for GenomePlex Whole Genome Amplification from Buccal Swab
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: CleanSEQ the ABI

(Date:1/15/2014)... 15, 2014 Freeslate, Inc ., ... announced that Lupin Limited, one of India’s top five ... Protégé PharmD System for high throughput solid form ... focused on a wide range of quality, affordable generic ...
(Date:1/14/2014)... Histogen, Inc., a regenerative medicine company ... under simulated embryonic conditions, today announced that they have ... Inc. for physician-dispensed aesthetic products containing Histogen’s proprietary multipotent ... is an amendment to the existing license between Histogen ...
(Date:1/14/2014)... BETHESDA, Md. , Jan. 14, 2014  Organovo ... institutes from the National Institutes of Health (NIH) to ... more effective treatments to patients on a faster timeline. ... Translational Sciences (NCATS) and the National Eye Institute (NEI) ...
(Date:1/14/2014)... Kinaxis ®, provider of RapidResponse ®, a cloud-based supply ... ( S&OP ) service, is proud to be a gold ... held at the Hilton San Diego Bayfront, January 30 – ... Kaszas , Director of Supply Chain at Amgen, as she ...
Breaking Biology Technology:Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 2Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 3Organovo Announces Collaboration with National Institutes of Health 2Organovo Announces Collaboration with National Institutes of Health 3Event Alert: Kinaxis Customer to Present at the Biomanufacturing Summit "Supply Chain: Improving Network Effectiveness" 2
... steps towards producing Influenza A (H1N1) VLP vaccine for animal ... Novavax, Inc. (Nasdaq: NVAX ) and the ... Institute of Allergy and Infectious Diseases (NIAID), National Institutes of ... evaluation of a virus-like particle (VLP) vaccine candidate against the ...
... June 4 EUCODIS Bioscience, a company,developing enzymes ... today that it has successfully launched its first,commercial ... EUCODIS,Bioscience and can be supplied in a GMP-compliant ... manufacturers to improve,quality control, to the diagnostics industry, ...
... SYDNEY, June 3 /PRNewswire-Asia/ -- Pharmaxis (ASX: ... had raised A$47 million through a placement to,Australian and ... company,s balance sheet as Pharmaxis moves toward,the European and ... , Pharmaxis will issue 20 ...
Cached Biology Technology:Novavax and the NIH Agree to Evaluate a Virus-like Particle (VLP) Vaccine Candidate Against the Novel Influenza A (H1N1) Virus 2Novavax and the NIH Agree to Evaluate a Virus-like Particle (VLP) Vaccine Candidate Against the Novel Influenza A (H1N1) Virus 3Novavax and the NIH Agree to Evaluate a Virus-like Particle (VLP) Vaccine Candidate Against the Novel Influenza A (H1N1) Virus 4EUCODIS Bioscience Launches Beta-Lactamase for Antibiotics and Diagnostics Industries 2Pharmaxis Announces Placement of $47 Million and Share Purchase Plan 2
(Date:4/24/2014)... Washington State University reveals how youth who work ... and the evidence lies in kids, saliva. ... Psychological Association,s Human-Animal Interaction Bulletin this ... a prevention perspective," said Patricia Pendry, a developmental ... under the skin" and the effects of prevention ...
(Date:4/24/2014)... University researchers are the first to sequence the genome ... eagle features that could lead to more effective conservation ... assumptions about golden eagle vision, indicating that the raptors ... previously thought. The genome also suggests that golden eagles ... realized. , Additionally, the genome provides thousands of ...
(Date:4/24/2014)... has been published in the Scientific Reports journal. ... Biology (a joint centre of CSIC and Pompeu Fabra University-UPF), ... decoded and we saw that it did not match with ... to sequence the complete genome and to make a functional ... belong to Louis XVI". , The functional genome analysis ...
Breaking Biology News(10 mins):'Horsing around' reduces stress hormones in youth 2'Horsing around' reduces stress hormones in youth 3Genome yields insights into golden eagle vision, smell 2The blood preserved in the pumpkin did not belong to Louis XVI 2
... 2010 Scientists at The Scripps Research Institute have solved the ... difficult to culture in the laboratory, providing scientists with useful ... when stem cells can be used for therapeutic purposes. ... Proceedings of the National Academy of Sciences (PNAS) during ...
... The world,s largest antimony mine has become the world,s ... antimony -- an element whose environmental and biological properties ... emergent contaminant," said IU Bloomington Ph.D. student Faye Liu, ... attention to it." Used in small quantities, antimony ...
... professor at NJIT, sees the use of terahertz rays ... bombers and other terrorist activities. Federici and his ... video camera invented in their laboratory that uses a ... could be used to scan airport passengers quickly and ...
Cached Biology News:Scripps Research scientists solve mystery of fragile stem cells 2Scripps Research scientists solve mystery of fragile stem cells 3A different kind of mine disaster 2A different kind of mine disaster 3NJIT physicist sees terahertz imaging as ultimate defense against terrorism 2NJIT physicist sees terahertz imaging as ultimate defense against terrorism 3
... On-Demand Cytometry The Guava ... blue laser based multiparameter cytometer with built-in ... you can screen plates of cells and ... of manipulating multiple tubes or having the ...
Methylation analysis of gene promoters using bilsulfite treatment of DNA and comparative DNA sequence analysis. Probe design and PCR included....
... Galaxy LIMS, Sophisticated Bioinformatics , Proprietary primer ... offer individual sample sequencing of PCR products, ... or double strand publication quality. Primer ... interest is 1.3 to 7 kilobytes long. ...
GLP-compliant, CLIA-certified DNA sequencing service. ASQ and SQA certified quality assurance staff. Auditors welcome....
Biology Products: