Navigation Links
Cis-Reporting System Monitors Intracellular Calcium Mobilization

human T-cell line, or CHO cells were transfected with the pNFAT-Luc plasmid and treated with 60 ng/ml of PMA and 1g/ml of inomycin, luciferase activity increased by 13- and 16-fold, respectively (Figure 2). Therefore, the enhancer element in the pNFAT-Luc plasmid is responsive to calcium mobilization. Cells transfected with pNFAT-Luc and then treated with either PMA or ionomycin alone did not show a significant increase in luciferase expression (result not shown), which is consistent with previous reports.2

Fig.2

Cyclosporin is an immunosuppressive drug that was introduced in 1983 and revolutionized transplant surgery. It works primarily by inhibiting the activity of calcineurin, a protein phosphatase regulated by intracellular calcium mobilization. All the isoforms of NFAT protein contain a calcineurin-binding domain and are activated by calcineurin. Results in Figure 2 demonstrated that the luciferase expression from pNFAT-Luc in both Jurkat and CHO cells induced by PMA and ionomycin could be completely inhibited by cyclosporin. In another set of experiments, rat basophilic leukemia cells stably transfected with chemokin receptors were transfected with pNFAT-Luc and then treated with their respective ligands (data not shown). When both luciferase expression and calcium levels were monitored in these cells, luciferase expression correlated very well with calcium mobilization. Therefore, luciferase expression from pNFAT-Luc indeed reflects the activation of endogenous NFAT proteins by calcium immobilization.

Conclusions

The pNFAT-Luc plasmid is a new addition to Stratagenes PathDetect cis-reporting systems. Stratagene has developed
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Cis-Reporting System Monitors Intracellular Calcium Mobilization
2. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
3. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
4. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
5. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
6. Enhanced PCR Cloning System
7. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
8. Performance Comparisons of Commercial RT-PCR Systems
9. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
10. Yeast Protein Production System Features High Yields and One-Step Purification
11. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
Post Your Comments:
(Date:1/22/2015)... , Jan. 22, 2015  Transwestern | RBJ today announces the ... office space for Shire a leading biopharmaceutical company, at Two ... | RBJ,s Robert Richards , president, and Brian ... for the entire five-floor building at 95 Hayden Ave. ...
(Date:1/22/2015)... The laboratory information management systems market ... number of technological advancements due to factors such as ... to integrate healthcare systems, and increasing government support for ... integration. Key players in the market focus on technological ...
(Date:1/22/2015)... / Rootstown, OH (PRWEB) January 22, 2015 ... platform, announced today that it has received AOAC-PTM Certifications for ... O1O3, O111, O121, and O145; collectively referred to as STEC ... O157, at 1 colony forming unit (cfu) per 325 g ...
(Date:1/22/2015)... zFlo Motion , a US leader in biomechanics ... from a new partner. STT-Systems has developed an ... years, with offerings in the optical-motion capture, 3D scanning, and ... measurement unit), iSen, is opening eyes around the world. With ...
Breaking Biology Technology:Transwestern?RBJ Advises Shire in 202,000 SF Lease, Creating Boston's Largest Suburban Biotech Campus 2Laboratory Information Management Systems Market is Expected to Reach $1,323.6 Million by 2019 - New Research Report by MarketsandMarkets 2Laboratory Information Management Systems Market is Expected to Reach $1,323.6 Million by 2019 - New Research Report by MarketsandMarkets 3Laboratory Information Management Systems Market is Expected to Reach $1,323.6 Million by 2019 - New Research Report by MarketsandMarkets 4Crystal Diagnostics Awarded AOAC-PTM Accreditation for the Rapid Detection of “Big 6” E.coli Food Pathogens 23D Motion Capture Just Got Easy 2
... , EATONTOWN, N.J. , Jan. 22 Osteotech, ... of biologic products for regenerative healing, announced today that its Board ... designed to discourage coercive or unfair takeover tactics and to provide ... the event that an unsolicited offer is made to acquire the ...
... Twenty leading US research institutions, patient advocates and biomedical industry ... 2009 Recovery and Reinvestment Act , WASHINGTON , ... anniversary of the Recovery and Reinvestment Act,s enactment on ... patient advocates and biomedical industry have released a detailed report ...
... ... QCam imaging driver for QImaging cameras. This interface provides support for Microsoft,s 64-bit ... faster analysis. , ... (PRWEB) January 20, 2010 -- QImaging, a leading developer and manufacturer of high-performance, ...
Cached Biology Technology:Osteotech, Inc. Adopts Stockholder Rights Plan 2Osteotech, Inc. Adopts Stockholder Rights Plan 3A Recovery Act Success Story: NIH Funding of Biomedical Innovation Is Creating Jobs, Fueling Industry Growth, and Helping Reduce Long-term Health Care Costs 2A Recovery Act Success Story: NIH Funding of Biomedical Innovation Is Creating Jobs, Fueling Industry Growth, and Helping Reduce Long-term Health Care Costs 3QImaging Introduces 64-Bit QCam Driver 2
(Date:1/22/2015)...  A man-made form of insulin delivered by nasal spray ... adults with mild cognitive impairment and Alzheimer,s disease dementia, ... Wake Forest Baptist Medical Center. The study,s ... impairment (MCI) or mild to moderate Alzheimer,s dementia (AD). ...
(Date:1/22/2015)... , Europe,s most prestigious innovation prize ... present a video retrospective of the most exciting and innovative nominations  ... and Sophie Wilson   A different theme ... Starting on 22 January 2015 on   http://www.epo.org/european-inventor   ...
(Date:12/24/2014)... 2014  Since its launch in December 2014, the 1U™ ... pain of trying to remember their usernames and passwords through ... their smartphones. To assist people who have struggled to remember ... created 1U and focuses on redefining identity, announced today that ...
Breaking Biology News(10 mins):Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 2Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 3Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 2Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 31U Offers Best Solution to the Username / Password Dilemma: For FREE! 21U Offers Best Solution to the Username / Password Dilemma: For FREE! 3
... the genetic basis of bipolar disorder, a highly heritable ... depression. The research, published by Cell Press online February ... , identifies a previously unrecognized susceptibility factor for bipolar ... to systematically sort through all the DNA of many ...
... All night long, bats swoop over our landscape consuming ... view. Until recently, scientists have been unable to bring ... genetic techniques, researchers from the University of Bristol and Biodiversity ... environment supporting these elusive creatures. Working at three ...
... With the steep decline in populations of many animal species, scientists ... extinction like those that have occurred just five times during the ... three-quarters or more of all animal species go extinct. In ... Nature , researchers report on an assessment of where mammals ...
Cached Biology News:Scientists identify susceptibility factor for bipolar disorder 2Food forensics: DNA links habitat quality to bat diet 2Earth's sixth mass extinction: Is it almost here? 2Earth's sixth mass extinction: Is it almost here? 3Earth's sixth mass extinction: Is it almost here? 4
0.2ml Gradient Block MBSR. Robot compatible block ejection mechanism. Stackable. (96 well block - holds 1 plate 0.2ml x 96 well plate)...
A Highly Purified, Cost-Effective Taq DNA Polymerase...
... Synonym: Dinitro-5,5'-dithiodibenzoic acid , DTNB , ... 396.36 g/mol , *CAS-No.: 69-78-3 ... a product with a reactive grouping not present ... include a reagent which will react directly with ...
Cag-A (IgG) -Ab EIA Sample Size: 10 l...
Biology Products: