Navigation Links
Cis-Reporting System Monitors Intracellular Calcium Mobilization

orting system includes the transfection-ready pNFAT-Luc reporter plasmid and the pCIS-CK negative control plasmid.

Construction of the pNFAT-Luc Plasmid

Fig.1

The backbone of the 5749-base-pair pNFAT-Luc plasmid (Figure 1) is the pFR-Luc reporter plasmid of the PathDetect trans-reporting system. To this backbone, we replaced the GAL4 binding element with four direct repeats of the NFAT binding sequence (286 to 257) from the IL-2 gene promoter, the most studied and widely used NFAT binding sequence.1,2,10,11 In addition to a polyadenylation signal downstream of the luciferase gene, the pNFAT-Luc plasmid contains another polyadenylation signal in front of the NFAT binding sites and TATA box. This design minimizes transcription read through from upstream sequences and background luciferase expression. For all reporter plasmids of the PathDetect cis-reporting systems, activation of the luciferase gene indicates interaction of uncharacterized gene products, extracellular stimuli, growth factors, or drug candidates with specific enhancer elements. When a plasmid expressing the gene of interest is cotransfected into mammalian cells along with a cis-reporter plasmid, luciferase activity indicates transcription activation.

Testing the pNFAT-Luc Plasmid in Jurkat Cells

Under natural cellular conditions, NFAT proteins are activated by antigen stimulation of the T-cell receptor.1 Pharmacology studies have established that NFAT proteins can be activated by the protein kinase C activator phorbol ester (PMA) in combination with the calcium ionophore ionomycin, reagents that raise free intracellular calcium.2,12 When Jurkat cells, a mature
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Cis-Reporting System Monitors Intracellular Calcium Mobilization
2. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
3. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
4. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
5. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
6. Enhanced PCR Cloning System
7. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
8. Performance Comparisons of Commercial RT-PCR Systems
9. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
10. Yeast Protein Production System Features High Yields and One-Step Purification
11. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
Post Your Comments:
(Date:9/15/2014)... 15, 2014 Applied Silicone Corporation, ... for the medical device and critical healthcare market, ... the implantable medical device market. The Dual ... delivering a silicone gel with improved fracture resistance, ... technology consists of two crosslinked networks working in ...
(Date:9/15/2014)... 15 septembre 2014 La deuxième édition ... (semaine internationale sur la sensibilisation au plasma) ... conjointe de la Plasma Protein Therapeutics Association ... , l,IPAW vise à : , ... la collecte du plasma-aphérèse , Reconnaître la ...
(Date:9/15/2014)... -- The second annual International Plasma Awareness Week (IPAW) ... of the Plasma Protein Therapeutics Association (PPTA) ... to: , Raise global awareness about source ... donors in saving and improving lives , Increase ... Member companies are planning a variety ...
(Date:9/14/2014)... UMBC’s Biotechnology Graduate Programs’ ... 21, 2014, from 12:00 to 1:00 p.m. EDT. The ... curriculum and practice-oriented instruction, designed for working professionals. Participants ... advance their career in the biotechnology industry. , If ... , About UMBC’s Biotechnology Graduate Programs, UMBC offers a ...
Breaking Biology Technology:Applied Silicone Launches Silicone Gel System with Dual Matrix Technology 2L' International Plasma Awareness Week célébrera les donneurs et braquera les projecteurs sur les maladies rares 2L' International Plasma Awareness Week célébrera les donneurs et braquera les projecteurs sur les maladies rares 3International Plasma Awareness Week To Celebrate Donors And Spotlight Rare Diseases 2International Plasma Awareness Week To Celebrate Donors And Spotlight Rare Diseases 3UMBC Biotechnology Graduate Programs’ Virtual Info Session, October 21, 2014 2
... (Nasdaq: ONXX ) today announced that it ... on Thursday, June 26, at,10:45 a.m. Eastern Time. ... presentation on our website at:, http://www.onyx-pharm.com/wt/page/event_calendar ... on 15 minutes early in order to,register and ...
... in London on Friday, June 20, 2008, NEW ... of the,leading plaintiffs, litigation law firms in America, filed ... a New York City Fire,Department Emergency Medical Technician whose ... EMT Ernest Armstead, was one,of the many brave responders ...
... Calif., June 19 The,Fifth Annual Healthcare Unbound ... Innovation (TCBI), will feature the coming together of,consumer ... help manage,diseases. The Conference will take place at ... will include a diverse representation of the many,business ...
Cached Biology Technology:Weitz & Luxenberg Files Seroquel Diabetes Lawsuit Against AstraZeneca 2Fifth Annual Healthcare Unbound Conference to Focus on Remote Monitoring, Home Telehealth & eHealth for Disease Management & Wellness Promotion 2Fifth Annual Healthcare Unbound Conference to Focus on Remote Monitoring, Home Telehealth & eHealth for Disease Management & Wellness Promotion 3
(Date:9/15/2014)... the subsequent rapid decrease in the water level of ... square kilometres of new, fertile land in eastern Finland. ... researchers has studied the role that the decrease in ... and humans. After dramatic shifts in the waterways, human ... rise to a new, innovative culture. This stemmed from ...
(Date:9/15/2014)... weighing as much as 170 tons, the blue whale is ... heaviest living thing ever seen on Earth. So there,s no ... fish larvae, which measure millimeters in length and tip the ... Not so fast, says L. Mahadevan, the Lola England de ... and of Physics. , Using simple hydrodynamics, a team ...
(Date:9/15/2014)... cardiovascular disease (CVD) claims 17 million lives each year, ... 9.4 million. 1 New research presented by international ... Montpellier, France on September 15, 2014, suggests that milk ... , At the Milk and Dairy Products in ... risk for hypertension and CVD was examined by Dr. ...
Breaking Biology News(10 mins):The creation of the Vuoksi River preceded a significant cultural shift 2The science behind swimming 2The science behind swimming 3Dairy consumption linked to lower blood pressure and cardiovascular disease risk 2
... research into cheetah cub survival has refuted the theory that ... cannot coexist in conservation areas. The study, published in the ... Kgalagadi Transfrontier Park were seven times more likely to survive ... found to be the cubs, main predatory threat. Previously, ...
... MA -- Researchers at MIT and Brigham and Women,s ... of intestinal stem cells, then stimulate them to develop ... intestinal cells. Using these cells, scientists could develop and ... colitis. The small intestine, like most other body ...
... approach to mapping how proteins interact with each other, ... aid in the design of new drugs for diseases ... artificial amino acids, the Salk scientists determine the detailed ... the molecule that turns it on. The switch--corticotrophin releasing ...
Cached Biology News:Researchers unlock a new means of growing intestinal stem cells 2Researchers unlock a new means of growing intestinal stem cells 3Salk scientists crack riddle of important drug target 2Salk scientists crack riddle of important drug target 3
... Prostaglandin F2α isopropyl ester (15(R)-17-phenyl trinor ... isomer containing both a double bond ... hydroxyl group at C-15. Similar to ... is a potential impurity in most ...
ACS Reagent Grade C 19 H 14 O 5 S Formula Weight: 354.38 PRODUCT SPECIFICATIONS Form: Rust/red crystalline powder Clarity of Solution: Passes test Visual Transition Interval: pH 6.8 - Yellow pH 8.2...
... Mouse Anti Nop1p/Fibrillarin (monoclonal) ... nucleolar protein of bakers yeast, Saccharomyces ... is essential for yeast viability and ... human homologue of Nop1p is fibrillarin ...
... G bind specifically to Fc regions of ... G conjugates are commonly used as affinity ... subtypes from serum, hybridoma ascites fluids, tissue ... reagents are also commonly used to capture ...
Biology Products: