Navigation Links
Choose the Right Transfection Agent for Your RNAi Experiment

I>Silencer Express (Mouse U6) siRNA Expression Cassette Kit and then transfected into HepG2, COS-7, MCF-7, HeLa, and DU145 cells with siPORT XP-1. Gene silencing effects were monitored by Northern blot 72 hours after transfection. RNA was isolated with the RNAqueous Kit, and Northern blots were performed using the NorthernMax-Gly Kit (Ambion).


Transfecting siRNAs into a Broad Range of Cell Types -- siPORT Amine and siPORT Lipid
In contrast to the DNA based expression systems for siRNA described above, many researchers perform transient RNAi experiments by direct transfection of siRNAs into cells. siPORT Amine (a polyamine mixture) and siPORT Lipid (a mixture of cationic and neutral lipids) are the transfection agents of choice for introducing siRNAs into a wide variety of mammalian cultured cells (Figure 4). We have found that different cell types are preferentially transfected with one or the other transfection agent. For instance, siPORT Lipid provides higher transfection efficiencies in HeLa cells, whereas siPORT Amine gives higher transfection efficiencies in 293 cells. Both agents demonstrate low cytotoxicity and are ideal for introducing individual siRNAs prepared by chemical synthesis or in vitro transcription, as well as siRNA populations prepared by digestion of long dsRNA by RNase III or Dicer.
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Multichannel Pipetting: How to Choose the Correct Pipettor
2. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
3. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
4. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
5. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
6. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
7. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
8. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
9. High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
10. Efficient Transfection of Neurospora Crassa
11. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Choose the Right Transfection Agent for Your RNAi Experiment

(Date:1/23/2015)... 23, 2015 Portal Solutions , a ... on Microsoft Office 365 and SharePoint platforms , today ... program for being one of the region’s fastest-growing mid-sized companies ... growth. , “To be recognized a second year in ...
(Date:1/22/2015)... , Jan. 22, 2015  Transwestern | RBJ today announces ... of office space for Shire a leading biopharmaceutical company, at ... Transwestern | RBJ,s Robert Richards , president, and ... lease for the entire five-floor building at 95 Hayden Ave. ...
(Date:1/22/2015)... January 22, 2015 Pipette.com has added ... to their comprehensive portfolio of Eppendorf products. , The ... purchase a Centrifuge 5424/5424 R and receive the following:, ... Eppendorf Research plus or Eppendorf Reference 2 , ...
(Date:1/22/2015)... (PRWEB) January 22, 2015 Diagenode, ... such as the Bioruptor® and complete solutions for ... for chromatin immunoprecipitation, alleviating the need for manual ... controls needed for ChIP of histones or transcription ...
Breaking Biology Technology:Washington SmartCEO Announces Portal Solutions as 2015 Future 50 Award Winner Second Year in a Row 2Washington SmartCEO Announces Portal Solutions as 2015 Future 50 Award Winner Second Year in a Row 3Transwestern?RBJ Advises Shire in 202,000 SF Lease, Creating Boston's Largest Suburban Biotech Campus 2Eppendorf Announces Promotional Bundle Which Includes: Eppendorf Centrifuge 5424, 3-Pack of Pipettes, and Tips - Available Now at Pipette.com 2Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2
... The new discovery by Aalto University can have major ... photo detectors and drug delivery. In bulk size, ... In nanoparticle size, the same material can be compressed ... discovery was made by an international research group led ...
... 2011 For pharmaceutical manufacturers, delays in starting a clinical ... trial material (CTM) and the associated fill/finish and ... wince in pain. A new, free white paper recommends a ... ( CMO ) to deliver all the required processes ...
... SILVER SPRING, Md., Aug. 24, 2011 United ... today the completion of its FREEDOM-C(2) Phase 3 ... sustained release oral formulation of treprostinil, a stable ... arterial hypertension (PAH).  Preliminary analysis demonstrates that the ...
Cached Biology Technology:New White Paper Examines Clinical Trial Material Production & Testing: What Your Contract Manufacturing Organization is Not Telling You 2New White Paper Examines Clinical Trial Material Production & Testing: What Your Contract Manufacturing Organization is Not Telling You 3FREEDOM-C(2) Trial of Oral Treprostinil in Pulmonary Arterial Hypertension Does Not Meet Primary Endpoint 2FREEDOM-C(2) Trial of Oral Treprostinil in Pulmonary Arterial Hypertension Does Not Meet Primary Endpoint 3FREEDOM-C(2) Trial of Oral Treprostinil in Pulmonary Arterial Hypertension Does Not Meet Primary Endpoint 4
(Date:1/22/2015)... Wis. , Jan. 15, 2015  BellBrook Labs, ... drug discovery, announced the launch of a TR-FRET ... the company,s Transcreener UDP Assay, a high throughput ... The new assay will allow for sensitive detection ...
(Date:1/22/2015)... , January 22, 2015 , ... tenth year  The European Patent Office to present a ... Two British nominations to be featured: Christofer Toumazou and ... featuring former finalists and winners of the Award   ...
(Date:12/22/2014)... 2014  NXT-ID, Inc. (NASDAQ: NXTD and NXTDW) ... the growing mobile commerce market, announces it has retained famous ... the 2015 International CES debut of the Wocket™ biometric smart ... at the NXT-ID booth January 6th and 7 th , ...
Breaking Biology News(10 mins):BellBrook Labs Launches a High Throughput Screening Assay for Glycosyltransferases with TR-FRET Readout 2Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 2Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 3NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 2NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 3NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 4
... Sea cucumbers are the champions of organ regrowth because ... organs, according to research published in the online open ... glaberrima uses similar cellular mechanisms during wound healing and ... to repair our own wounds and, perhaps eventually, how ...
... Laurette Dub, James McGill Chair in Consumer and ... Health Challenge Think Tank based on the firm ... comprehensive, effective way demands a massive mobilization across ... end, this years event will draw a Whos ...
... they have just confirmed a controversial theory of evolution. ... the origin of new species., Biologists have argued for ... animalsplays a special role in the process of speciation. ... Daven Presgraves, professor of biology at the University of ...
Cached Biology News:Sea cucumbers fast track organ regrowth by healing their wounds 2Changing the global dietary environment 2X-effect: female chromosome confirmed a prime driver of speciation 2
Cyan Standard is 7-hydroxy-4-trifluoromethylcoumarin...
6-(2,4-dinitrophenyl)aminohexanoic acid, succinimidyl ester (DNP-X, SE)...
Endothelin-converting enzyme 1 (EC 3.4.24.71) (ECE-1). [Source:Uniprot/SWISSPROT;Acc:P42892] Antigen: Recombinant Protein Epitope Signature Tag (PrEST)....
...
Biology Products: