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Choose the Right Transfection Agent for Your RNAi Experiment

I>Silencer Express (Mouse U6) siRNA Expression Cassette Kit and then transfected into HepG2, COS-7, MCF-7, HeLa, and DU145 cells with siPORT XP-1. Gene silencing effects were monitored by Northern blot 72 hours after transfection. RNA was isolated with the RNAqueous Kit, and Northern blots were performed using the NorthernMax-Gly Kit (Ambion).


Transfecting siRNAs into a Broad Range of Cell Types -- siPORT Amine and siPORT Lipid
In contrast to the DNA based expression systems for siRNA described above, many researchers perform transient RNAi experiments by direct transfection of siRNAs into cells. siPORT Amine (a polyamine mixture) and siPORT Lipid (a mixture of cationic and neutral lipids) are the transfection agents of choice for introducing siRNAs into a wide variety of mammalian cultured cells (Figure 4). We have found that different cell types are preferentially transfected with one or the other transfection agent. For instance, siPORT Lipid provides higher transfection efficiencies in HeLa cells, whereas siPORT Amine gives higher transfection efficiencies in 293 cells. Both agents demonstrate low cytotoxicity and are ideal for introducing individual siRNAs prepared by chemical synthesis or in vitro transcription, as well as siRNA populations prepared by digestion of long dsRNA by RNase III or Dicer.
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