Navigation Links
Choose the Right Transfection Agent for Your RNAi Experiment

iple Cell Lines with an siRNA Expression Plasmid Transfected with siPORT XP-1. pSilencer 2.0-U6 was engineered to express an siRNA to GAPDH and then transfected into COS-7, HeLa, and DU145 cells with siPORT XP-1. 72 hours after transfection, RNA was isolated with the RNAqueous Kit and GAPDH mRNA levels were analyzed by Northern blot using Ambion's NorthernMax-Gly Kit. NC=no construct, ie cells transfected with transfection agent alone.


Transfecting PCR products, such as those prepared using Ambion's Silencer Express siRNA Expression Cassette Kit, can be challenging as many plasmid transfection agents are not suitable for transfection of these shorter, linear dsDNAs. However, XP-1 is also efficient at introducing SECs and other PCR products into a range of mammalian cell types. Protocols for transfecting SECs are distinct from those for transfecting plasmid expression vectors and are outlined in a separate section of the siPORT XP-1 Protocol. Figure 3 shows the use of siPORT XP-1 for transfecting SECs targeting GAPDH into five different cell lines. GAPDH mRNA levels were reduced by >90% in three of the five cell lines, and >75% in the remaining two.

Figure 3. SECs Delivered with siPORT XP-1 Induce Gene Silencing. siRNA Expression Cassettes (SECs) were prepared with the <
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Multichannel Pipetting: How to Choose the Correct Pipettor
2. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
3. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
4. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
5. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
6. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
7. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
8. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
9. High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
10. Efficient Transfection of Neurospora Crassa
11. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Choose the Right Transfection Agent for Your RNAi Experiment

(Date:5/23/2013)... Calif. , May 23, 2013  Saddleback Memorial ... recently became the first hospital on the West ... technology. This new surgical option provides women with a ... Marc Winter , M.D., a ... and surgeon and medical director of minimally invasive surgery ...
(Date:5/23/2013)... , May 23, 2013 Z Trim Holdings, ... ingredients to a variety of industries, announced that Chef ... panel discussion at this year,s National Restaurant Association ("NRA") ... on behalf of the Research Chefs Association.  Chef Ryan ... The Good, the Bad and the Ugly," joining four ...
(Date:5/23/2013)... , May 23, 2013  Verenium Corporation (Nasdaq: ... on the development and commercialization of high-performance enzymes, today ... Financial Officer, will present at two upcoming conferences. ... will present at the Second Annual Marcum LLP Microcap ... Hyatt Hotel in New York City.  The presentation is ...
(Date:5/23/2013)... 23, 2013 African Ancestry ... for people of African descent, today announced new ... For people of all races, myDNAmix determines various ... distinct populations: Indigenous Americas (Native American), East Asia, ... , Originally launched in late 2011, ...
Breaking Biology Technology:Saddleback Memorial Surgeon First on the West Coast to Perform Single-Site Robotic Hysterectomy 2Saddleback Memorial Surgeon First on the West Coast to Perform Single-Site Robotic Hysterectomy 3Z Trim's Chef Erin Ryan sits on Expert Panel at NRA Show 2Z Trim's Chef Erin Ryan sits on Expert Panel at NRA Show 3Verenium To Present At Two Upcoming Conferences 2Verenium To Present At Two Upcoming Conferences 3AfricanAncestry.com Rolls Out Improved MyDNAMix Admixture Test for People of all Races; Enhancements Increase Accuracy in Estimation of Ancestral Makeup 2AfricanAncestry.com Rolls Out Improved MyDNAMix Admixture Test for People of all Races; Enhancements Increase Accuracy in Estimation of Ancestral Makeup 3
... Oct. 27 /Xinhua-PRNewswire/ -- Recently, China Green,Material ... Intellectual,Property Office for two patents for Industrialized ... and Industrialized Production,Method of Fully Biodegradable Edible ... applications have both obtained acceptance numbers,200810064592 and ...
... Trubion Pharmaceuticals Inc.,(Nasdaq: TRBN ) today ... study,demonstrating that repeat administration with TRU-015 continues ... rheumatoid arthritis,(RA) signs and symptoms based on ... announced positive results following preliminary,analyses from a ...
... Inc. ("Carna"),announced on October 27, 2008, the signing ... ("SUBC"). Under the terms of this,Agreement, SUBC will ... China., With China,s emergence as a strong ... companies have now established,research and production facilities in ...
Cached Biology Technology:Patent Technologies Keep CGMT in a Leading Position in the Industry 2Trubion Reports Re-treatment Results Following Phase 1/2a and Phase 2b Studies Evaluating TRU-015 for Rheumatoid Arthritis 2Trubion Reports Re-treatment Results Following Phase 1/2a and Phase 2b Studies Evaluating TRU-015 for Rheumatoid Arthritis 3Trubion Reports Re-treatment Results Following Phase 1/2a and Phase 2b Studies Evaluating TRU-015 for Rheumatoid Arthritis 4Trubion Reports Re-treatment Results Following Phase 1/2a and Phase 2b Studies Evaluating TRU-015 for Rheumatoid Arthritis 5Carna Biosciences Signs Distribution Agreement with Shanghai Universal Biotech Company 2
(Date:5/23/2013)... In an age when microbial pathogens are growing ... down infection, a team of Wisconsin scientists has synthesized ... the bacteria that cause staph infections. , Writing online ... , a group led by University of Wisconsin-Madison chemistry ... the "quorum sensing" behavior of Staphylococcus aureus , ...
(Date:5/22/2013)... join together in a major new effort to educate ... providing Earth,s people with clean water that looms ahead ... comment article in the current edition of Chemical ... American Chemical Society (ACS), the world,s largest scientific society. ... and Jerald L. Schnoor, Ph.D., explain that shortages of ...
(Date:5/22/2013)... feared Legionella pneumophila bacteria is responsible for ... In order to infect us, this pathogen has developed ... go unnoticed in our cells, thus avoiding these acting ... Basque biosciences research centre, CIC bioGUNE, in which teams ... USA and the National Supercomputation Centre in Barcelona (BSC) ...
Breaking Biology News(10 mins):University of Wisconsin chemists find new compounds to curb staph infection 2Mechanism discovered which aids Legionella to camouflage itself in the organism 2
... these larvae of tiger salamanders used as live bait ... hope to catch largemouth bass, channel catfish and other ... than they bargained for: salamanders in bait shops in ... and those in Arizona, with a chytrid fungus called ...
... BIO-key International,s MobileCop(R) and DaProSystems, Public Safety System ... a Link to DispatchersWALL, N.J., April 6 ... BKYI), a leader in finger-based biometric identification and ... of Roanoke, Virginia, a leading provider of Public ...
... Drilling Program (IODP), the world,s largest ocean research ... scientific support by welcoming Australia, India, and New ... New Zealand,s membership is agreed upon in a ... Lead Agencies: Japan,s Ministry of Education, Culture, Sports, ...
Cached Biology News:Spring fishing season arrives... and with it, amphibian diseases 2Spring fishing season arrives... and with it, amphibian diseases 3Nine Additional Virginia Law Enforcement Agencies Deploy BIO-key(R) and DaPro Systems Solution 2Nine Additional Virginia Law Enforcement Agencies Deploy BIO-key(R) and DaPro Systems Solution 3Nine Additional Virginia Law Enforcement Agencies Deploy BIO-key(R) and DaPro Systems Solution 4Australia, India, New Zealand join integrated ocean drilling program 2
... cells were cultured in Ham's F12K medium with ... phase of growth. In order to keep the ... in acetone-methanol. The cells are arrayed on a ... wells surface specifically treated to enhance cellular attachment ...
Mouse polyclonal antibody to REXO4 - XPMC2 prevents mitotic catastrophe 2 homolog (Xenopus laevis)...
... Microsphere/Microarray Stabilizer is an aqueous solution that ... non-toxic chemicals in a PBS buffer (phosphate ... This product contains a combination of 0.02% ... StabilCoat Plus Stabilizer provides optimum performance ...
... The mini incubation trays ... used to screen antigens that ... membrane. Each tray has eight ... channels that accommodate strips cut ...
Biology Products: