Navigation Links
Choose the Right Transfection Agent for Your RNAi Experiment

he presence or absence of serum. siPORT XP-1 is simple to use -- reagent/DNA complexes can be added directly to growing cells in serum-containing culture media, and the media can be left unchanged until the time of assay.

An example of the plasmid transfection efficiencies that can be obtained with siPORT XP-1 is shown in Figure 1. In this figure, a plasmid designed to express -galactosidase was transfected into HeLa cells using siPORT XP-1 and the cells were analyzed by microscopy. Transfection rates of 70-80% have been achieved with HeLa cells as shown by the blue staining.

Figure 1. siPORT XP-1 Efficiently Delivers Plasmid DNA. HeLa cells were transfected with a plasmid expressing -galactosidase using siPORT XP-1. 48 hours after transfection, the cells were treated with X-gal and analyzed by microscopy. The blue color indicates -galactosidase expression.


In gene silencing experiments, high transfection efficiencies are essential in order to observe the down regulation of genes by Northern or Western analysis, or any other technique that analyzes the entire cell population, containing both transfected and nontransfected cells. In Figure 2, siPORT XP-1 was used to transfect COS-7, HeLa, and DU145 cells with a plasmid, pSilencer 2.0-U6, engineered to express an siRNA to GAPDH. Silencing effects were analyzed at the mRNA level by Northern blot 72 hours after transfection. In all three cell lines, GAPDH mRNA levels were reduced >80%, demonstrating the effectiveness of plasmid transfections by siPORT XP-1 in these three cell lines.

Figure 2. Gene Silencing in Mult
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Multichannel Pipetting: How to Choose the Correct Pipettor
2. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
3. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
4. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
5. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
6. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
7. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
8. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
9. High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
10. Efficient Transfection of Neurospora Crassa
11. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Choose the Right Transfection Agent for Your RNAi Experiment

(Date:7/29/2014)... a new way of manufacturing microstructured surfaces that ... self-assembly of carbon nanotubes, could exhibit a variety ... and strength, or the ability to repel water ... that mechanical forces can be used to direct ... we can independently control the mechanical properties ...
(Date:7/29/2014)... 29, 2014  Discovery Laboratories, Inc. (Nasdaq: DSCO),  ... new standard of respiratory critical care, will report its ... 2014 before the open of the U.S. financial ... a live teleconference and webcast at 9:00 a.m. Eastern ... Discovery Labs, management will discuss the 2014 second ...
(Date:7/29/2014)... CO (PRWEB) July 29, 2014 TransVac ... soiled linen collection and transport solutions for hospitals, will ... for Healthcare Engineering (ASHE) Annual Conference & Technical Exhibition ... will present its technology for making hospitals cleaner, reducing ... costs over the hospital’s life cycle. Staff will ...
(Date:7/29/2014)... 2014 DisasterTalk is a texting app which ... phone masts or other transmitters are down. The app can ... without the need for any Wi-Fi, and can also use ... new app has launched as a housing report ... high risk of being affected by natural disasters. With the ...
Breaking Biology Technology:A new way to make microstructured surfaces 2Discovery Labs to Report 2014 Second Quarter Financial Results and Hold Conference Call on Tuesday, August 5, 2014 at 9:00 a.m. Eastern Time 2TransVac Presents Cost Saving Technology at ASHE Conference for Keeping Hospitals Cleaner 2DisasterTalk App Could Save Lives as Report Reveals 8% of US Homes at Very High Risk of Natural Disaster 2
... September 25 XTL,Biopharmaceuticals Ltd. (NASDAQ: XTLB ; ... Meeting ("AGM") and Extraordinary General Meeting,("EGM") was postponed for ... to the absence of a quorum. The postponed AGM ... Kiryat Weizmann Science Park,Rehovot, Israel 76100 at 4:00 pm ...
... REHOVOT, Israel, Sept. 25 ,Monsanto Company (NYSE: ... announced,a collaboration to improve nitrogen use efficiency in ... gains exclusive rights to,a number of genes discovered ... applications of nitrogen. Monsanto will work to evaluate ...
... Plc, a,private biopharmaceutical company focusing on first-in-class, ... appointed Dr.,Johnson Lau and Dr. David Sidransky ... Johnson Lau (MBBS, MD, FRCP) brings a ... corporate fields, having a distinguished,background in both ...
Cached Biology Technology:XTL Biopharmaceuticals Ltd. AGM / EGM Postponed Until October 2, 2007 2Monsanto and Evogene Collaborate on Nitrogen Use Efficiency Research 2Monsanto and Evogene Collaborate on Nitrogen Use Efficiency Research 3Morria Biopharmaceuticals Plc Appoints Dr. Johnson Lau and Dr. David Sidransky to Board of Directors 2
(Date:7/29/2014)... bacteria and algae, propel themselves using whip-like appendages ... a millimetre long, function like tiny oars, helping ... cilia are found on the surfaces of many ... liquids over the cell. , Flagella and ... expel pathogens from our airways, they establish the ...
(Date:7/28/2014)... by the University of Arizona has sequenced the complete ... enhance scientists, and agriculturalists, understanding of the growing patterns ... of new rice varieties that are better able to ... hunger challenges. , The paper, "The genome sequence of ... independent domestication," was published online in Nature Genetics ...
(Date:7/28/2014)... A study of juvenile rat brain cells suggests that ... the connections between brain cells are temporary. , The ... PLOS ONE , was conducted by biologists at ... Medical College in New York in response to concerns, ... decade, that exposing children to general anesthetics may increase ...
Breaking Biology News(10 mins):Microscopic rowing -- without a cox 2Microscopic rowing -- without a cox 3Generating a genome to feed the world: UA-led team sequences African rice 2Generating a genome to feed the world: UA-led team sequences African rice 3Generating a genome to feed the world: UA-led team sequences African rice 4Study suggests disruptive effects of anesthesia on brain cell connections are temporary 2Study suggests disruptive effects of anesthesia on brain cell connections are temporary 3
... Salk Institute Laboratory of Genetics and holder of the ... Neurodegenerative Diseases, has been named a 2011 recipient of ... Research Projects (T-R01) program. Only 17 T-R01 projects ... with a total of $15.9 million in funding. Gage, ...
... has been published in the journal Coral Reefs by Giacomo Bernardi, ... Santa Cruz. In the video, an orange-dotted tuskfish digs ... rock, and repeatedly throws the clam against the rock to crush ... "What the movie shows is very interesting. The animal excavates sand ...
... be linked to the decline of water calcium levels ... that helps keep blooms at bay. Daphniaalso known ... on algae and keeping it in check. However, without ... water calcium levels get low and Daphnia populations decrease ...
Cached Biology News:Video shows tool use by a fish 2Loss of 'lake lawnmowers' leads to algae blooms 2
... Bruker Daltonics and Bruker BioSpins ... for metabolism studies, and analysis of ... ESI-TOF system, an optional Avance NMR ... acquisition, evaluation and statistical analysis for ...
... Morris Water MazeRecords animal movements in a ... subjects at once in a predefined sequence of ... instruments all built around a video camera, frame ... track two fields at once and up to ...
... Records behavior directed toward examining a novel object ... family of instruments all built around a video ... that can track two fields at once and ... extracts animal positions and records them, but also ...
... Grade C 19 H 9 Br 4 ... SPECIFICATIONS Form: Powder Clarity of Solution: Passes ... - Yellow pH 4.6 - Blue ... agarose and acrylamide gel electrophoresis. Used in ...
Biology Products: