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Choose the Right Transfection Agent for Your RNAi Experiment


The success of any RNA interference experiment depends on the efficient delivery of siRNA or siRNA expression vehicles (plasmids, PCR fragments) into cells. Many researchers use cationic liposomal and polyamine based reagents to deliver these molecules into cultured mammalian cells. The choice of reagent and the conditions under which it is used depend on the type of cells and the type of molecule being transfected. Reagents optimized for delivery of plasmid DNA are not optimal for delivering siRNA, and vice versa. Furthermore, few transfection agents intended for plasmid DNA transfection work well for transfecting PCR derived siRNA expression cassettes (SECs: PCR fragments expressing siRNAs).

Ambion has performed extensive research on the reagents and conditions needed for introducing siRNA, siRNA expression plasmids, and SECs into cells. Of all of the reagents tested, two for siRNAs and one for plasmids and SECs consistently gave the best transfection efficiencies for the widest number of cell lines tested. These reagents are now provided by Ambion for transfecting mammalian cultured cells (see below).


Efficient Delivery of Plasmids and SECs -- siPORT XP-1
siPORT XP-1 is an easy-to-use reagent that efficiently delivers both plasmid DNA and PCR products into a variety of mammalian cell types. Comprised of a proprietary formulation of polyamines, the reagent exhibits low toxicity and can be used in t
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