Navigation Links
Characterizing galvanizing baths

Automobile-parts production can be paralyzed by a defective electron-galvanizing (EV) bath line. Because a sensitive and accurate analysis of EV solutions is fundamental to productivity, quality, and identification of errors, a detailed analytical profile can help trace the source of error and contribute to selecting the best solution. Both downtime and material cost therefore can be minimized.

Fluorescence spectroscopy is an analytical technique with high sensitivity and selectivity. By characterizing the components in the EV-coat solution with fluorescence, a detailed analytical profile can be formed for each component. This can help trace the source of errors in the EV-coat solution, and contribute to selection of the best formulation. Quality-control tests can be preformed periodically to check for consistent bath composition.

The SPEX FLUOROMAX spectrofluorometer was used in this investigation. The system is compact, economical, and offers several automated accessories. The FLUOROMAX is noted for its outstanding sensitivity, speed, and easy-to-use software.
High sensitivity is achieved by means of all-reflective optics and photon-counting detection.
Data acquisition is fast because the system can slew at 160 nm s1. Coupling sensitivity and rapid data acquisition creates a system that can function as an efficient and productive laboratory instrument.
Complete experiments can be stored in memory and recalled by a technician for push-button, menu-driven operation.

Therefore, spectra can be acquired in seconds, and a time-based scan can be collected at 1 ms/data point. Simplicity is apparent in the automation, computer-controlled variable slits, software, and calibration. For convenience, all of the controls are located in the keyboard of a PC-compatible computer.

Experimental Procedures
Samples obtained for analysis were divided into six segments. Electron-galvanizing bath (EV) was a sample of the current bath causing problems for the operator. Sample A is the plating bath that would compose the ideal EV bath. Sample B is a bath that contains 0.6 mL of starter and 1.5 mL of brightener. Sample C is a bath that contains 2 mL of starter and 0.5 mL of brightener. Starter and brightener stock samples were obtained for comparison.

Results and Discussion
The six samples were characterized by their excitation and emission spectra shown in Figures 1 through 4. The fluorescence spectra could offer a possible method for correctly mixing the appropriate concentrations or proportion of starter and brightener solutions in the EV bath. For the coating process to work, a constant level of starter and brightener is necessary.

Figure 1 illustrates that sample A was 5 105 counts lower in fluorescence intensity than the EV bath. Sample C was 106 counts higher in intensity than the EV bath. Therefore, a component in sample C must have been the cause of the greater intensity. Sample C contained the largest amount of starter solution, the smallest volume of brightener solution. This indicates that the actual EV bath contained too much starter solution, based on the difference in fluorescence intensities.

The data were fitted to the following linear equation:

If =A [Brightener] +B [Starter] +C

where A, B, and C are constants, [Brightener] is the concentration of brightener, [Starter] is the concentration of starter, and If is the observed intensity of fluorescence. An empirical linear relationship thus was established between the fluorescence intensity and ra tio of brightener to starter:

This equation is merely a simple form of chemometrics. Larger, more complex experimental analyses can be handled with the use of a more elaborate form of chemometrics.

back to top


Page: All 1 2 3

Related biology technology :

1. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
2. Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer
3. Sensitive detection of tumor cells in peripheral blood of carcinoma patients by a reverse transcription PCR method
4. Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer
5. Mutation detection for the K- rasand P16 genes
6. Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager
7. The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists
8. TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization
9. cAMP DataFile: Homogenous Cyclic AMP detection using High Efficiency Fluorescence Polarization
10. Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis
11. Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.
Post Your Comments:

(Date:11/25/2015)... LOS ANGELES and HOLLISTON, Mass. ... Regenerative Technology, Inc. (Nasdaq: HART ), a biotechnology ... announced that CEO Jim McGorry will present ... Tuesday, December 1, 2015 at 2:30 p.m. PT. The ... (link below) for 30 days. Management will also be ...
(Date:11/25/2015)... ... November 25, 2015 , ... A long-standing ... Aerospace Professionals (OPBAP) has been formalized with the signing of a Memorandum of ... with OPBAP leaders Capt. Karl Minter and Capt. Albert Glenn Tuesday, November 24, ...
(Date:11/25/2015)... (PRWEB) , ... November 25, 2015 , ... ... company uBiome, were featured on AngelList early in their initial angel funding process. ... AngelList syndicate for individuals looking to make early stage investments in the microbiome ...
(Date:11/24/2015)... , Nov. 24, 2015 Cepheid (NASDAQ: ... speaking at the following conference, and invited investors to ... NY      Tuesday, December 1, 2015 at 11.00 ... NY      Tuesday, December 1, 2015 at 11.00 ... Conference, New York, NY      Tuesday, ...
Breaking Biology Technology:
(Date:11/26/2015)... 26, 2015 Research and Markets ( ... Fingerprint Sensors - Technology and Patent Infringement Risk Analysis" ... --> --> Fingerprint sensors using capacitive ... The fingerprint sensor vendor Idex forecasts an increase of ... mobile devices and of the fingerprint sensor market between ...
(Date:11/19/2015)... MOUNTAIN VIEW, Calif. , Nov. 19, 2015 /PRNewswire/ ... authentication market, Frost & Sullivan recognizes BIO-key with the ... Strategy Leadership. Each year, Frost & Sullivan presents this ... comprehensive product line catering to the needs of the ... which the product line meets and expands on customer ...
(Date:11/18/2015)... new scientific discoveries deepen our understanding of how cancer ... challenges in better using that knowledge to guide treatment ... children continue to survive pediatric cancer, that counseling may ... John M. Maris, M.D ., a pediatric oncologist ... --> John M. Maris, M.D ., ...
Breaking Biology News(10 mins):