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Characterization of Protein Oligosaccharides Using MSn

l integrity of the m/z 1087.8 ion is maintained regardless of its pathway of formation.


Figure 5. MS2-7 for stepwise removal of terminal GlcNAc residues and formation of the more stable GlcNAc2Man3(OH)6 core substrate, m/z 1087.7.



Figure 6. Determination of GlcNAc8Man3 glycan branching patterns by MS6.

Conclusions

Electrospray ion trap mass spectrometry is a particularly effective method for detailed characterization of protein oligosaccharides. The MSn capabilities of the LCQ Series overcome the significant limitations often encountered with traditional triple quadrupole techniques. By exploiting unique saccharide losses, MSn enables the isolation and analysis of individual glycan species, even in the presence of an isobaric contaminant. The removal of especially labile substituents in early stages of MSn produces stable ion substrates which, upon further MSn analysis, yield spectra that are far more structurally informative than those obtained by MS/MS.

References

1. Reinhold, V.N., Reinhold, B.B., Chan, S., Methods Enzymol., 271, 377, (1996).
2. Weiskopf, A.S.; Vouros, P.; Harvey, D.J. Rapid Commun. Mass Spectrom., 11, 1493,(1997).
3. Ciucanu, I.; Kerek, F. Carbohydr. Res., 131, 209, (1984).
4. Nomenclature system from Domon, B.; Costello C.E. Glycoconj, J. 5, 397, (1988).




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