Navigation Links
Characterization of Protein Oligosaccharides Using MSn

l integrity of the m/z 1087.8 ion is maintained regardless of its pathway of formation.


Figure 5. MS2-7 for stepwise removal of terminal GlcNAc residues and formation of the more stable GlcNAc2Man3(OH)6 core substrate, m/z 1087.7.



Figure 6. Determination of GlcNAc8Man3 glycan branching patterns by MS6.

Conclusions

Electrospray ion trap mass spectrometry is a particularly effective method for detailed characterization of protein oligosaccharides. The MSn capabilities of the LCQ Series overcome the significant limitations often encountered with traditional triple quadrupole techniques. By exploiting unique saccharide losses, MSn enables the isolation and analysis of individual glycan species, even in the presence of an isobaric contaminant. The removal of especially labile substituents in early stages of MSn produces stable ion substrates which, upon further MSn analysis, yield spectra that are far more structurally informative than those obtained by MS/MS.

References

1. Reinhold, V.N., Reinhold, B.B., Chan, S., Methods Enzymol., 271, 377, (1996).
2. Weiskopf, A.S.; Vouros, P.; Harvey, D.J. Rapid Commun. Mass Spectrom., 11, 1493,(1997).
3. Ciucanu, I.; Kerek, F. Carbohydr. Res., 131, 209, (1984).
4. Nomenclature system from Domon, B.; Costello C.E. Glycoconj, J. 5, 397, (1988).




'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing
2. Isolation and Characterization of Anti-Viral Nucleoside Compounds from the Chinese Herb Taraxacum Mongolicum
3. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
4. Characterization of tooth decay
5. Sciclone ALH 3000 Liquid Handler: Functional Characterization of the High Volume Head - Part 1: Plate-to-Plate Precision Testing
6. A New C-Terminal GST Vector for Protein Production in S. pombe
7. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
8. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
9. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
10. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
11. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Characterization Protein Oligosaccharides Using MSn

(Date:7/24/2014)... CA (PRWEB) July 24, 2014 ... reporting of molecular diagnostic test results. It also ... necessary for the validation and reporting of high ... Molecular diagnostics is a rapidly growing area of ... disease, genetic predisposition, cancer, and companion diagnostics in ...
(Date:7/23/2014)... July 23, 2014  Sangamo BioSciences, Inc. (NASDAQ: ... financial results and accomplishments. For the ... consolidated net loss of $7.0 million, or $0.10 per ... or $0.10 per share, for the same period in ... cash, cash equivalents, marketable securities and interest receivable of ...
(Date:7/23/2014)... Helmholtz-Zentrum Dresden-Rossendorf (HZDR), the Vienna University of Technology ... in embedding nearly perfect semiconductor crystals into a ... hybrid nanowires, very fast and multi-functional processing units ... the future. The research results will be published ... Nano-optoelectronics are considered the cornerstone of future chip ...
(Date:7/23/2014)... July 23, 2014 Shimadzu Scientific ... amino acid analysis system developed jointly with Ajinomoto ... Station can analyze 38 amino acids in nine ... Station achieves high-speed analysis utilizing a fast application-specific ... provides accurate analytical results even under conditions with ...
Breaking Biology Technology:New DNA-barcode Patent Virtually Eliminates False Test Results and Sample Mix-up 2Sangamo BioSciences Reports Second Quarter 2014 Financial Results 2Sangamo BioSciences Reports Second Quarter 2014 Financial Results 3Sangamo BioSciences Reports Second Quarter 2014 Financial Results 4Sangamo BioSciences Reports Second Quarter 2014 Financial Results 5Sangamo BioSciences Reports Second Quarter 2014 Financial Results 6Sangamo BioSciences Reports Second Quarter 2014 Financial Results 7Sangamo BioSciences Reports Second Quarter 2014 Financial Results 8Sangamo BioSciences Reports Second Quarter 2014 Financial Results 9A crystal wedding in the nanocosmos 2Shimadzu's New UF-Amino Station Provides High-Speed, Multi-Component Analysis of Amino Acids in Food & Beverage and Pharmaceutical Testing 2
... RICHMOND, Va. (Sept. 1, 2009) An international team ... that acts as a semiconductor and could help material ... microchips. The team of researchers from Virginia Commonwealth ... of Science in Shanghai, China; and Tohoku University in ...
... , , ... Transgenomic, Inc. (OTC Bulletin Board: TBIO) and Power3 Medical Products, ... on-line publication of a clinical research paper entitled "Abnormal Serum ... Biochemical and Biophysical Research Communications. The study demonstrates the usefulness ...
... , , , BOULDER, ... LENA Autism Screen (LAS) to 91 percent for children 24 to 48 ... is now as accurate or more accurate than other autism screens currently ... http://www.newscom.com/cgi-bin/prnh/20090210/LA69114 ) , , "We,re thrilled with this leap ...
Cached Biology Technology:Researchers design new graphene-based, nano-material with magnetic properties 2Transgenomic and Power3 Medical Report Identification of Abnormal Serum Proteins in Parkinson's Disease 2Transgenomic and Power3 Medical Report Identification of Abnormal Serum Proteins in Parkinson's Disease 3Transgenomic and Power3 Medical Report Identification of Abnormal Serum Proteins in Parkinson's Disease 4Advancement Made in Automatic Autism Screen Increases Accuracy to 91%, Scheduled for Release This Month 2Advancement Made in Automatic Autism Screen Increases Accuracy to 91%, Scheduled for Release This Month 3
(Date:7/24/2014)... -- To reduce fire hazard in the United States, ... cutting woody shrubs and suppressed trees (ladder fuels). ... during periods of low fire danger in order to ... minimize hazardous air pollutants, managers often cover all or ... commonly referred to as agricultural plastic, in order to ...
(Date:7/24/2014)... BRUNSWICK, N.J. A yearlong study funded by the ... the Edward J. Bloustein School of Planning and Public ... owners in Sandy-affected towns are skeptical about the likelihood ... online survey respondents, 45 percent indicated they were "pessimistic" ... affected by Superstorm Sandy would be rebuilt better than ...
(Date:7/24/2014)... the primordial soup in which life arose have been maintained ... of East Anglia. , Research published today in the ... yeast and very likely also in animals still perform ancient ... life some four billion years ago. , The primordial ... ocean as a result of the combination of metals, gases ...
Breaking Biology News(10 mins):Study gives new perspective on agricultural plastic, debris burning, and air quality 2Rutgers study explores attitudes, preferences toward post-Sandy rebuilding 2
... dozens of shark species living in the coastal waters off ... the species, but a survey begun nearly 25 years ago ... populations and their role in marine ecosystems. NOAA scientists ... R.I., recently conducted their ninth coastal shark survey from Florida ...
... colorful leaves adorning the trees are a delight to the ... the United States and East Asia boast lustrous red foliage. ... autumnal hues around the world? A new theory provided by ... at the University of Haifa-Oranim and Prof. Jarmo Holopainen of ...
... team of researchers from Boston University, Harvard Medical School ... that changes a long held paradigm about how bacteria ... that causes human stomach ulcers uses a clever biochemical ... allowing it to move and survive and further colonize ...
Cached Biology News:Scientists conduct shark survey off US East Coast 2Scientists conduct shark survey off US East Coast 3Why are autumn leaves red in America and yellow in Europe? 2Why are autumn leaves red in America and yellow in Europe? 3Uncovering the secrets of ulcer-causing bacteria 2
...
... cytostatic and anti-viral agent. Inhibits several important steps ... formation of HUVEC cells. Also reduces intracellular reactive ... IL-8 release of endothelial cells. ... 16 O 8 MolWeight: ...
... includes the pZErO-1 vector with Zeocin resistance ... vector utilizes positive selection to eliminate high ... resistance gene reduces the overall vector size ... kit contains all of the reagents you ...
NATIVE, ENZYME PORCINE TRYPSIN...
Biology Products: