l integrity of the m/z 1087.8 ion is
maintained regardless of its pathway of formation.
Figure 5. MS2-7 for stepwise removal of terminal GlcNAc residues and formation of the more stable GlcNAc2Man3(OH)6 core substrate, m/z 1087.7.
Figure 6. Determination of GlcNAc8Man3 glycan branching patterns by MS6.
Electrospray ion trap mass spectrometry is a
particularly effective method for detailed characterization
of protein oligosaccharides. The MSn
capabilities of the LCQ Series overcome the
significant limitations often encountered with
traditional triple quadrupole techniques. By
exploiting unique saccharide losses, MSn
the isolation and analysis of individual glycan species, even in the presence of an isobaric
contaminant. The removal of especially labile
substituents in early stages of MSn
ion substrates which, upon further MSn
yield spectra that are far more structurally informative
than those obtained by MS/MS.
1. Reinhold, V.N., Reinhold, B.B., Chan, S., Methods Enzymol., 271, 377, (1996).
2. Weiskopf, A.S.; Vouros, P.; Harvey, D.J. Rapid Commun. Mass Spectrom., 11, 1493,(1997).
3. Ciucanu, I.; Kerek, F. Carbohydr. Res., 131, 209, (1984).
4. Nomenclature system from Domon, B.; Costello C.E. Glycoconj, J. 5, 397, (1988).
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