Navigation Links
Challenge the Performance of Your Hot-Start PCRs,,,with FastStart Taq DNA Polymerase and,,,the Novel FastStart High Fidelity PCR System

The FastStart Taq DNA Polymerase

FastStart Taq DNA Polymerase, a chemically modified version of Taq DNA Polymerase for hot start PCR, was introduced in 2000. Based on the feedback from many customers, FastStart Taq DNA Polymerase has proven to be: The most robust enzyme preparation currently available
Even the most challenging PCRs are no longer a problem. In applications where other polymerases stop working, FastStart Taq DNA Polymerase gives effective results (Figures 1, 2, and 3).

The most versatile enzyme preparation currently available

FastStart Taq DNA Polymerase is also the enzyme of choice for real-time quantitative PCR, e.g., as applied on the LightCycler Instrument. It is also included in most LightCycler System reagent kits.

The FastStart High Fidelity PCR System
The novel FastStart High Fidelity PCR System contains the new FastStart High Fidelity Enzyme Blend: a new blend of FastStart Taq DNA Polymerase and a thermostable proofreading protein that mediates proofreading activity, but has no polymerase activity itself.
This enzyme blend is inactive at temperatures below 75C and does not elongate nonspecific primer-template hybrids that may form during PCR setup. After activation via a two-minute incubation step at 95C, the processivity of FastStart Taq DNA Polymerase, and the ability of the proofreading protein to recognize and excise incorrectly incorporated nucleotides, combine to provide hot-start amplification of a variety of DNA and cDNA targets up to 5 kb with

    high specificity,
    high sensitivity and yield, and
    approximately fourfold higher fidelity compared to Taq DNA Polymerase and FastStart Taq DNA Polymerase.
Therefore, the FastStart High Fidelity Enzyme Blend is of particular use when

    larger fragments (up to 5 kb) need to be amplified with the highest possible specificity, sensitivity, and yield (Figure 4).
    proofreading polymerases or chemical modifications thereof fail to amplify fragments up to 5 kb.
    the application is difficult and challenging (Figures 5 and 6).

Both FastStart Taq DNA Polymerase and the FastStart High Fidelity PCR System allow you to

Improve the efficiency of your lab
    Develop and establish new assays quickly; testing many different enzyme preparations is no longer required.
    Save time by reducing cumbersome optimization steps.
    Improve existing assays to get the most out of the PCR technology.
Save money
    Reduce the number of different enzymes in your laboratory enzyme stock.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. The Best Enzyme for the Toughest PCR Challenges Improved with New Hot-Start Feature
2. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
3. Comparing Fidelity and Performance of Proofreading PCR Enzymes
4. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
5. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
6. Performance Comparisons of Commercial RT-PCR Systems
7. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
8. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Highly Sensitive microRNA Array Performance
11. High Throughput RNA Isolation for High Performance Microarray Analysis
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/12/2016)... SAN DIEGO , Feb. 12, 2016 Biocom, ... life science community, took a group of San ... as part of its 2016 Precision Medicine Advocacy Fly-In. ... at the Food and Drug Administration (FDA), the Centers for ... (NIH), as well as San Diego U.S. Representatives Susan ...
(Date:2/11/2016)... , February 11, 2016 Non-profit Consortium ... Genetic Understanding to Support Research and Discovery ... today announced an ambitious plan to sequence 100,000 individuals. It ... countries and at least 7 of North and East Asian ... the first phase, the project will focus on creating phased ...
(Date:2/11/2016)... 2016  Spectra BioPharma Selling Solutions (Spectra) is ... biopharma companies the experience, expertise, operational delivery and ... sales teams. Created in concert with industry leading ... strategic and tactical needs of its clients by ... both personal and non-personal promotion. ...
(Date:2/11/2016)...   BioInformant announces the February 2016 release ... Opportunities, Tools, and Technologies – Market Size, Segments, Trends, ... The first and only market ... BioInformant has more than a decade of historical information ... stem cell type. This powerful 175 page global strategic ...
Breaking Biology Technology:
(Date:2/3/2016)... , February 4, 2016 --> ... SEK 1,351.5 M (105.0), up 1,187% compared with fourth quarter of 2014. ... to SEK 517.6 M (loss: 30.0). Earnings per share increased ... was SEK 537.4 M (neg: 74.7). , ... Revenues amounted to SEK 2,900.5 M (233.6), up 1,142% compared with 2014. ...
(Date:2/3/2016)... , Feb. 3, 2016 Vigilant Solutions announces ... Department in Missouri solved two ... reader (LPR) data from Vigilant Solutions. Brian ... in which the victim was walking out of a convenience store and witnessed ... next to his vehicle, striking his vehicle and leaving ...
(Date:2/2/2016)... Feb. 2, 2016  BioMEMS devices deployed ... focused on medical screening and diagnostic applications, ... Wearable devices that facilitate and assure continuous ... movement are being bolstered through new opportunities ... signal acquisition coupled with wireless connectivity and ...
Breaking Biology News(10 mins):