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Challenge the Performance of Your Hot-Start PCRs,,,with FastStart Taq DNA Polymerase and,,,the Novel FastStart High Fidelity PCR System

The FastStart Taq DNA Polymerase

FastStart Taq DNA Polymerase, a chemically modified version of Taq DNA Polymerase for hot start PCR, was introduced in 2000. Based on the feedback from many customers, FastStart Taq DNA Polymerase has proven to be: The most robust enzyme preparation currently available
Even the most challenging PCRs are no longer a problem. In applications where other polymerases stop working, FastStart Taq DNA Polymerase gives effective results (Figures 1, 2, and 3).

The most versatile enzyme preparation currently available

FastStart Taq DNA Polymerase is also the enzyme of choice for real-time quantitative PCR, e.g., as applied on the LightCycler Instrument. It is also included in most LightCycler System reagent kits.

The FastStart High Fidelity PCR System
The novel FastStart High Fidelity PCR System contains the new FastStart High Fidelity Enzyme Blend: a new blend of FastStart Taq DNA Polymerase and a thermostable proofreading protein that mediates proofreading activity, but has no polymerase activity itself.
This enzyme blend is inactive at temperatures below 75C and does not elongate nonspecific primer-template hybrids that may form during PCR setup. After activation via a two-minute incubation step at 95C, the processivity of FastStart Taq DNA Polymerase, and the ability of the proofreading protein to recognize and excise incorrectly incorporated nucleotides, combine to provide hot-start amplification of a variety of DNA and cDNA targets up to 5 kb with

    high specificity,
    high sensitivity and yield, and
    approximately fourfold higher fidelity compared to Taq DNA Polymerase and FastStart Taq DNA Polymerase.
Therefore, the FastStart High Fidelity Enzyme Blend is of particular use when

    larger fragments (up to 5 kb) need to be amplified with the highest possible specificity, sensitivity, and yield (Figure 4).
    proofreading polymerases or chemical modifications thereof fail to amplify fragments up to 5 kb.
    the application is difficult and challenging (Figures 5 and 6).

Both FastStart Taq DNA Polymerase and the FastStart High Fidelity PCR System allow you to

Improve the efficiency of your lab
    Develop and establish new assays quickly; testing many different enzyme preparations is no longer required.
    Save time by reducing cumbersome optimization steps.
    Improve existing assays to get the most out of the PCR technology.
Save money
    Reduce the number of different enzymes in your laboratory enzyme stock.



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