Challenge the Performance of Your Hot-Start PCRs,,,with FastStart Taq DNA Polymerase and,,,the Novel FastStart High Fidelity PCR System
The FastStart Taq DNA Polymerase
FastStart Taq DNA Polymerase, a chemically modified version
of Taq DNA Polymerase for hot start PCR, was introduced
in 2000. Based on the feedback from many customers,
FastStart Taq DNA Polymerase has proven to be: The most robust enzyme preparation
Even the most challenging PCRs are no longer a problem.
In applications where other polymerases stop working,
FastStart Taq DNA Polymerase gives effective results
(Figures 1, 2, and 3).
The most versatile enzyme preparation
FastStart Taq DNA Polymerase is also the enzyme of
choice for real-time quantitative PCR, e.g., as applied on
the LightCycler Instrument. It is also included in most
LightCycler System reagent kits.
The FastStart High Fidelity PCR System
The novel FastStart High Fidelity PCR System contains
the new FastStart High Fidelity Enzyme Blend: a new
blend of FastStart Taq DNA Polymerase and a
thermostable proofreading protein that mediates proofreading
activity, but has no polymerase activity itself.
This enzyme blend is inactive at temperatures below
75C and does not elongate nonspecific primer-template
hybrids that may form during PCR setup. After activation
via a two-minute incubation step at 95C, the processivity
of FastStart Taq DNA Polymerase, and the ability
of the proofreading protein to recognize and excise
incorrectly incorporated nucleotides, combine to provide
hot-start amplification of a variety of DNA and cDNA
targets up to 5 kb with
high sensitivity and yield, and
approximately fourfold higher fidelity compared to
Taq DNA Polymerase and FastStart Taq DNA
Therefore, the FastStart High Fidelity Enzyme Blend is of
particular use when
larger fragments (up to 5 kb) need to be amplified
with the highest possible specificity, sensitivity, and
yield (Figure 4).
proofreading polymerases or chemical modifications
thereof fail to amplify fragments up to 5 kb.
the application is difficult and challenging (Figures 5
Both FastStart Taq DNA Polymerase and the FastStart High Fidelity PCR System allow you to
Improve the efficiency of your lab
Develop and establish new assays quickly; testing many different enzyme preparations is no longer required.
Save time by reducing cumbersome optimization steps.
Improve existing assays to get the most out of the PCR technology.
Reduce the number of different enzymes in your laboratory enzyme stock.
Source:Page: All 1 2 Related biology technology :1
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