Navigation Links
Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter

ed in experimental samples.

Quench Correction
Figure 2 shows the 32P quench curve prepared with yellow food coloring as the quench agent. Quench monitoring was performed with tSIS (a spectrum based quench indicating parameter exclusive to the TopCount). Figure 2 shows that tSIS is capable of accurately monitoring quenching due to color in samples that are Cerenkov counted.

Kinase Assay
Figure 3 shows a plot comparing the Cerenkov counting results from the kinase assay on both the TopCount and LSC. Data was plotted as % of the maximum binding on the respective instrument. As mentioned earlier, specific levels of kinase activity were measured on TopCount using FlexiFilter. The individual filter spots were removed from the FlexiFilter and Cerenkov counted in a Packard Tri-Carb 2500TR LSC for comparison. As seen in Figure 3, both the LSC and TopCount plots are nearly identical which indicates that equivalent final results will be obtained for the assay.

Conclusions

Cerenkov counting is an attractive alternative for various TopCount applications using 32P.

An important advantage of Cerenkov counting on the TopCount is that it provides the ability to measure 32P labels directly in the microplate without cocktail. This makes stripping and reprobing of 32P labeled dot blots possible after counting. In addition, Cerenkov counting provides the ability to perform other molecular biology applications such as PCR product screening and labeling verification in a rapid and easy manner. Since cocktail addition is not required, throughput is increased because samples are counted directly.

For most microplate applications, the TopCount Cerenkov efficiency gives quantitative results and greatly reduces labor compared to Cerenkov co
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. Counting Cytostar-T scintillating microplates on the MicroBeta JET
3. Counting FlashPlates on the Wallac MicroBeta
4. Direct Counting of Millipore MultiScreen Filtration Plates.
5. Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter
6. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting
7. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
8. Comparing Fidelity and Performance of Proofreading PCR Enzymes
9. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
10. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
11. Performance Comparisons of Commercial RT-PCR Systems
Post Your Comments:
(Date:7/10/2014)... 10, 2014 Terascala, the industry leader ... Alan Swahn, a former vice president of product management ... vice president of marketing. Terascala’s software when combined with ... create the highest performance and most reliable solutions for ... guide Terascala’s channel expansion and broaden its product portfolio. ...
(Date:7/10/2014)... OMICS Group’s 5th International conference and exhibition ... at Double Tree by Hilton Beijing, China is all ... & Bioanalytical research methods only to facilitate improved living ... Dr. Srinubabu Gedela, MD of OMICS Group Inc has ... one in bringing a unique and international mix of ...
(Date:7/10/2014)... , July 10, 2014  Franciscan St. ... of capnography for respiratory monitoring outside the ... healthcare leaders in embracing state-of-the-art patient safety ... patients are breathing and can alert medical ... measuring the amount of carbon dioxide the ...
(Date:7/10/2014)... PROVO, Utah , July 10, 2014 ... genome annotation and interpretation, today announced an ... sequencing (NGS) analytics for Lineagen,s NextStep Dx ... evaluation of neurodevelopmental and neurological disorders, currently ... chromosomal microarray (CMA) testing service used by ...
Breaking Biology Technology:Terascala Expands Executive Team 2Terascala Expands Executive Team 3A Critical Review of Analytical Techniques for Improved Living Standards 2A Critical Review of Analytical Techniques for Improved Living Standards 3A Critical Review of Analytical Techniques for Improved Living Standards 4Franciscan St. Anthony Health-Crown Point Underscores Patient Safety Commitment Through Expanded Use of Capnography 2Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 2Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 3Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 4
... of the Telecommunications Professionals of Wisconsin is consolidating ... networking and business support organization focused on information ... "reflects the increasingly blurred line between the telecommunications ... in companies of all sizes and in every ...
... Green Bay soil-technology company that uses an all-encompassing method of ... Small Business Technology Transfer Award and the $5,000 prize that ... manufactures and distributes Advanced Soil Technology products, was presented the ... Early-Stage Symposium presented by the Wisconsin Technology Council ...
... dual role as director of information systems and chief financial ... Kathi Christian holds positions that sometimes are in conflict. , ... the natural tendency to evaluate the cost-benefit and return on ... an exercise in frustration. ROI can be difficult to measure ...
Cached Biology Technology:Accelerate Madison to merge with Telecommunications Professionals 2Green Bay's ENCAP wins tech transfer award 2CIO Leadership Series: Kathi Christian, QTI Group 2CIO Leadership Series: Kathi Christian, QTI Group 3CIO Leadership Series: Kathi Christian, QTI Group 4
(Date:7/11/2014)... including scientists from the Max Planck Institute for Medical ... step in understanding photosynthesis, the process by which the ... its atmosphere and which is therefore crucial for all ... researchers report the first direct visualization of a crucial ... which a specific protein complex, photosystem II, splits water ...
(Date:7/11/2014)... the hippocampus, the marginal division of the striatum ... is the impact degree of substance P in ... function? Yan Yu and his team, College of ... immunofluorescence staining, that substance P receptor, neurokinin 1 ... marginal division of normal rats. Unilateral or bilateral ...
(Date:7/11/2014)... Rio de Janeiro, Brazil- In the brains of ... mechanism that allows an electric or chemical signal ... another. Chemical synapses, which are the most abundant ... inhibitory. Synapse formation is crucial for learning, memory, ... and inhibitory synapses critical for brain function. ...
Breaking Biology News(10 mins):A first direct glimpse of photosynthesis in action 2Blame it on the astrocytes 2
... Boston, Mass.Researchers at Boston Children,s Hospital have found that ... could potentially protect the fragile lungs of premature babies ... by inflammation. The findings explain earlier research ... called mesenchymal stem cells (MSCs) could help reduce lung ...
... fast. That,s an essential characteristic of what makes them cancer ... continuously growing and dividing, far outstripping our normal cells. To ... CSHL Professor Adrian Krainer and his team have found ... in the process specifically kill cancer cells. Nearly 90 ...
... as regenerating damaged tissues or organs have generated a ... types of applications are exciting, it is a long journey ... cells on human health will most likely come from their ... to generate stem cells by reprogramming cells from patients, skin ...
Cached Biology News:Microscopic packets of stem cell factors could be key to preventing lung disease in babies 2Microscopic packets of stem cell factors could be key to preventing lung disease in babies 3Team uses antisense technology that exploits gene splicing mechanism to kill cancer cells 2Team uses antisense technology that exploits gene splicing mechanism to kill cancer cells 3The role of stem cells in developing new drugs 2
Mouse monoclonal antibody to ANAPC2 - anaphase promoting complex subunit 2...
...
... long and outstanding history in the ... ago we became aware that our ... modified to inject Xenopus oocytes. We ... microdispenser to eliminate any tip movement ...
The Programmable MP-3500P-F Power Supply can be applied for any Microfluidic application....
Biology Products: