Navigation Links
Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter

ical but is due to the highly penetrating radiation of 32P beta particle interacting with neighboring wells. The data also indicate that the type of filter medium influences counting efficiency. The Cerenkov counting efficiencies on TopCount for UniFilter and FlexiFilter are about 50% of that obtained by Cerenkov counting on an LSC. Higher counting efficiencies on the TopCount can be achieved at the expense of a slightly higher background by adjusting the lower discriminator to a lower value.

Microcentrifuge Tube Counting
Average counting efficiency for 0.4 mL tubes was approximately 13% for all volumes of water added from 10 μL to 400 μL. Backgrounds are less than 300 CPM. A fairly constant counting efficiency over the sample volume range of 10 to 1500 μL was observed for the 1.5 mL tubes. The lowest observed was 13% (1500 μL) and the highest was 17% (500 μL volume). Background for the 6-60 chosen window will be less than 300 CPM. Higher counting efficiency can be obtained at the expense of background by raising the lower discriminator setting.

Volume Effect
Table 2 shows the TopCount Cerenkov counting efficiency performance as a function of volume of water sample in a 96- and a 24-well OptiPlate. The data shown are the mean of three replicates.

The data show that the volume of water influences the counting efficiency in a 96-well plate with maximum counting efficiency at the largest sample size. There does not seem to be a significant volume effect in the 24-well format.

Performance Linearity
The performance linearity data are shown in Figure 1 which is a plot of TopCount CPM versus DPM activity. The DPM was determined by counting the same activity on a Packard Tri-Carb 2500TR LSC. The figure shows that response is linear throughout the entire range of activities most likely encounter
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. Counting Cytostar-T scintillating microplates on the MicroBeta JET
3. Counting FlashPlates on the Wallac MicroBeta
4. Direct Counting of Millipore MultiScreen Filtration Plates.
5. Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter
6. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting
7. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
8. Comparing Fidelity and Performance of Proofreading PCR Enzymes
9. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
10. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
11. Performance Comparisons of Commercial RT-PCR Systems
Post Your Comments:
(Date:1/14/2014)... DC (PRWEB) January 14, 2014 Carahsoft ... for Thursday, January 23, 2014 at 2pm EST (11am ... Knowledge.” The topic focuses on how technology can turn ... critical decisions for government agencies. The online webinar will ...
(Date:1/14/2014)... January 14, 2014 EquitiesIQ, a leading ... (OTCQB: ALQA). Alliqua is an emerging biomedical company acquiring, ... wound care market. , Free report download: http://equitiesiq.com/reports/alliqua/ ... a seasoned management team and Board, which launched the ...
(Date:1/14/2014)... 14, 2014 In recent years, growing ... methods in product development and promotion has led to ... mistrust, fueled by concerns about the insidious impact of ... of spectacular fines to the world’s biggest pharmas for ...
(Date:1/14/2014)... UK (PRWEB) January 13, 2014 Bob ... with more than 20 years in the industry, has ... photonics . Hainsey will serve as the society’s Science ... Dr. Hainsey join SPIE as our Technology Strategist, further ...
Breaking Biology Technology:Webcast - Natural Language Processing: Converting Raw Data into Actionable Knowledge – Hosted by Carahsoft and CDS Federal Services 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 3The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 2The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 3Photonics R&D Leader Bob Hainsey Joins SPIE Technical Staff 2
... , , Catalog Number, Pack ... 100 g, , , , , , ... German , version - 42 K , ... Material Safety , Data Sheets, , MSDS , ...
... , , , , , , , ... , 1691104, Set, ... Package , Inserts/Product Instructions , , English , ... - 24 K , , US Material Safety , Data Sheets , ...
... , Here are some troubleshooting hints that we have gathered , regarding misincorporation or low fidelity of PCR ... , , , , ... , , <a , ... , , , ...
Cached Biology Technology:Trouble-shooting: Misincorporation or low fidelity 2Trouble-shooting: Misincorporation or low fidelity 3
(Date:4/17/2014)... trials to treat tuberculosis could be the basis for ... bacteria, fungal infections and parasites, yet evade resistance, according ... collaborators. , Led by U. of I. chemistry professor ... drug SQ109 attacks the tuberculosis bacterium, how the drug ... to malaria and how targeting multiple pathways reduces ...
(Date:4/17/2014)... 17, 2014The development of stem cell therapies to cure ... characterize stem cell populations based on cell surface markers. ... new marker that is highly expressed in a type ... which they describe in an article in BioResearch ... Liebert, Inc., publishers. The article is available free on ...
(Date:4/17/2014)... Marc Andr Gauthier and Professor Luca Razzari of ... been awarded large grants from the John R. ... Innovation (CFI) for the acquisition of state-of-the-art biotech ... added matching grants from the Ministre de l,Enseignement ... de la Technologie (MESRST). These new laboratories will ...
Breaking Biology News(10 mins):Multitarget TB drug could treat other diseases, evade resistance 2New state-of-the-art biotech and nanotech equipment for INRS 2
... the body, a virus such as HIV invades body cells ... Once inside the cell, the invading microbe's genetic material takes ... more copies of the virus, which then spill out to ... that once a virus begins fusing with a cell's membrane, ...
... in prospect with a clinical drug trial to begin in ... would open the way to relief in the 10% of ... over 500,000,000 people. , The Walter and Eliza Hall ... Guinea Institute of Medical Research (PNGIMR) and the University of ...
... Medical Center (SFVAMC) and the University of California, San ... bronchitis, which is thought to lead to obliterative bronchitis ... transplanted lungs. , The researchers hope their results will ... standard test for chronic lung rejection, as well as ...
Cached Biology News:Several minute intermediate stage in virus-cell fusion discovered; opportunity for drug development 2Several minute intermediate stage in virus-cell fusion discovered; opportunity for drug development 3Old drug, new tricks: Prospects for slashing the impact of malaria 2Researchers identify genes associated with lung transplant rejection 2
... Labsystems Nepheloskan Ascent is the ... measurement in microplates. Nepheloskan measures ... ultra-sensitive technique for particle quantification. ... solubility testing in drug discovery, ...
... Crotalus adamanteus venom Description: Phosphodiesterase ... used in studying nucleic acid ... It hydrolyzes 5'-mononucleotides from 3'-hydroxy-terminated ... Phosphodiesterase I cleaves ADP-ribosylated proteins ...
Economical and manually filled LN2 vacuum insulated storage container offers high capacity and low nitrogen consumption while offering convenient storage of samples....
... Useful for the determination of digitonin and digoxin. ... EDTA Na 4 and 0.06% NaCl Preparation ... al., Biochem. Biophys. Res. Commun., 19, 755 ... mole of inorganic phosphorus from ATP per min ...
Biology Products: