Navigation Links
Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter

es.

Quench Correction
An experiment was performed to investigate the ability to correct for color quenching. A quench curve containing 151,000 DPM of 32P per well was prepared in a 96-well OptiPlate using yellow food coloring as the quench agent as described in TopCount Topics, TCA-015.5 The total volume of water per well was 200 μL.

Kinase Assays
A kinase assay was performed with the FlexiFilter to test the applicability of the Cerenkov counting technique with TopCount in a routinely performed filter based assay. A protein kinase C (PKC) assay kit (RPN.77, Amersham) was used in the experiment. This assay measures the amount of PKC in a sample by the degree of phosphorylation of a specific peptide with [32P]-ATP. The assay was performed essentially as described in TopCount Topics, TCA-018.4 Serial dilutions of PKC (P-8289, Sigma) were used to generate several levels of specific kinase activity ranging from 125 to 7.8 units of activity per well. Slight modifications were made in this assay compared to the one described in TCA-018. A 25 minute incubation instead of 15 minutes was performed; the stop reagent volume was reduced to 40 μL; and 15 μL instead of 10 μL of activity was spotted onto a precut sheet of phosphocellulose binding paper in a filtration manifold. After the assay was counted on TopCount, the individual sample spots were cut out from the filter and placed with 3 mL of water in glass 7 mL LSC vials and Cerenkov counted on a Packard Tri-Carb 2500TR liquid scintillation analyzer for comparison.

Results and Discussion

96- and 24-Well Microplates and Filter Plates
Table 1 shows the counting efficiency, energy crosstalk, and background CPM for a variety of microplate formats. Note that the crosstalk is not opt
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. Counting Cytostar-T scintillating microplates on the MicroBeta JET
3. Counting FlashPlates on the Wallac MicroBeta
4. Direct Counting of Millipore MultiScreen Filtration Plates.
5. Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter
6. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting
7. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
8. Comparing Fidelity and Performance of Proofreading PCR Enzymes
9. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
10. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
11. Performance Comparisons of Commercial RT-PCR Systems
Post Your Comments:
(Date:8/4/2015)... ... August 04, 2015 , ... On August 4, 2015, ... diverse selection of entrepreneurs from across the United States to talk about their experiences ... honored to be chosen to be among the attendees. , One of the day’s ...
(Date:8/4/2015)... Aug. 4, 2015   California Cryobank Stem Cell ... of LifeLine Cryogenics, an east coast cord blood and ... York tristate area. LifeLine clients will maintain ... contracts will be honored. Since 1991, LifeLine ... storage of sperm, eggs, embryos, and cord blood stem ...
(Date:8/4/2015)... Biotech Ltd. (Nasdaq: SVA ), a leading provider ... today announced that it will release its unaudited financial ... after market close on Wednesday, August 12, 2015. The Company ... 2015, at 8:00 a.m. EDT (Thursday, August 13, 2015 ... Company,s financial results and provide an update on recent ...
(Date:8/3/2015)... , Aug. 3, 2015  Halozyme Therapeutics, Inc. (NASDAQ: ... Call for the second quarter 2015 on Monday, August ... Helen Torley , president and chief executive officer, ... Halozyme will release financial results for the second quarter ... through the "Investors" section of Halozyme,s corporate website and ...
Breaking Biology Technology:White House Invites uBiome CEO Jessica Richman to First-Ever Startup Demo Day 2FamilyCord Announces Acquisition of LifeLine Cryogenics 2Sinovac to Host Conference Call to Report Second Quarter 2015 Unaudited Financial Results 2Halozyme to Host Second Quarter 2015 Financial Results Conference Call 2
... Isolation from E. coli Using the Eppendorf<sup , ... Plasmid Mini , Kit , , ... Joel Lopez, Melissa Higgins, Chere Griffin and Vince Prezioso, PhD , ... Introduction , Isolation and purification of plasmid DNA from bacteria ...
... or pathogens , Gabriela M. Tobal ,Stratagene ... Stratagene is introducing PCR primer sets ... these primers have been published previously,and are well ... use PCR technology to identify the presence of ...
... New PathDetect reporting system for ... Matthew Hsu Mary Buchanan Chao-Feng Zheng ... plasmid,the pNFAT-Luc reporter plasmid, is designed for studying ... (NFAT) transcription factors. These,interesting proteins, which play an ...
Cached Biology Technology:Comparison of Growth Techniques and Media for the Purpose of Plasmid , Isolation from E. coli Using the Eppendorf , Perfectprep Plasmid Mini , Kit 2Comparison of Growth Techniques and Media for the Purpose of Plasmid , Isolation from E. coli Using the Eppendorf , Perfectprep Plasmid Mini , Kit 3Comparison of Growth Techniques and Media for the Purpose of Plasmid , Isolation from E. coli Using the Eppendorf , Perfectprep Plasmid Mini , Kit 4New PCR Primers for Human Diseases 2New PCR Primers for Human Diseases 3New PCR Primers for Human Diseases 4Cis-Reporting System Monitors Intracellular Calcium Mobilization 2Cis-Reporting System Monitors Intracellular Calcium Mobilization 3Cis-Reporting System Monitors Intracellular Calcium Mobilization 4Cis-Reporting System Monitors Intracellular Calcium Mobilization 5
(Date:8/3/2015)... Calif. , Aug. 3, 2015 Synaptics, ... human interface solutions, today announced that members of the ... at the Pacific Crest Global Technology Leadership Forum on ... The conference will be held at the Sonnenalp Resort ... The presentation may include forward-looking information. An audio ...
(Date:7/31/2015)... 31. juli 2015 Den 10. internationale konference ... BGI fra den 22. - 25. oktober i ... sin 10-års fødselsdag i år. Siden starten i 2006 ... møder på ,omik,-feltet, og er en af de mest ... ICG-10 fokuserer på nylige gennembrud og fremskridt inden for ...
(Date:7/31/2015)... de 2015 BGI llevará a cabo la 10ª ... al 25 de octubre de 2015, en ... su décimo aniversario este año. Desde su inauguración en ... reuniones anuales más influyentes del mundo en los campos ... científicas más dinámicas, entusiastas y amenas. ICG-10 ...
Breaking Biology News(10 mins):Den 10. internationale konference om Genomics (ICG-10) åbner i oktober 2La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 2La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 3
... An international team of scientists has discovered striking similarities between ... a computer chip. The finding is reported in the journal ... compared to computers, but apart from the trivial fact that ... a physical space, it has been unclear whether this is ...
... AVIV, Israel , April 22, 2010 ... has co-authored a paper that brings together a myriad of ... kinetics. "Geometry-Controlled Kinetics," published in Nature Chemistry on April ... of a particular medium affects the motion of molecules or ...
... range of techniques for establishing crops in the previous ... surface. The principal benefits of conservation tillage are improved ... benefits can include reduced fuel consumption, planting and harvesting ... published in HortScience finds promise in a ...
Cached Biology News:Brains, worms and computer chips have striking similarities 2Tel Aviv University President Co-authors Important Paper Unraveling the Effect of Spatial Organization on Intracellular Chemistry 2Tel Aviv University President Co-authors Important Paper Unraveling the Effect of Spatial Organization on Intracellular Chemistry 3Cover crop mulches tested for no-till organic onions 2
Mouse monoclonal antibody raised against a partial recombinant CENTB2. NCBI Entrez Gene ID = CENTB2...
... The tubing prep station, 100-120 V, is ... with DNA-coated gold particles to create the source ... helium discharge with the Helios gene gun. The ... O-rings, tubing prep unit, 12 foot Nalgene nitrogen ...
Mouse polyclonal antibody to NR2F2 - nuclear receptor subfamily 2, group F, member 2...
The Discovery Series PDQuest 2-D analysis software is used for imaging, analyzing, and databasing two-dimensional electrophoretic gels. The 10-user network license entitles ten users to access data f...
Biology Products: