Navigation Links
Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter

Abstract

The TopCount Microplate Scintillation and Luminescence Counter has been widely used in biopharmaceutical assays involving both radionuclide and luminescence measurement. A new feature allows the use of TopCount for measuring a high energy beta emitter such as 32P by Cerenkov counting, a well-known measurement technique that has the main advantage of not requiring the addition of scintillation cocktail. Since cocktail is not required for measurement, there is little or no sample preparation, no chemical quenching, and the cost and time requirement to add cocktail is avoided. These advantages make Cerenkov counting an attractive alternative to liquid scintillation counting of 32P in microplate based assays. Cerenkov counting performance in the TopCount is documented for a variety of sample matrices and assays. Typical TopCount efficiency, background, and crosstalk data are presented for both liquid and filter based assays. Quench correction for colored samples is also demonstrated.

Introduction

Cerenkov radiation occurs when charged particles traveling at or greater than the speed of light pass through a sample medium (usually liquid) where there is an exchange of energy from the charged particle to the molecules of the medium.1 β energies in excess of 1 MeV are more desirable since the counting efficiencies are higher and closer to those observed by liquid scintillation counting. The main advantage to Cerenkov counting is the fact that scintillation cocktail is not required for measurement. The popularity of this technique is well documented for a variety of applications where higher energy beta emitters are used. For example, 32P (Emax= 1710 keV) is used in molecular biology and enzyme activity assays because of the ease by which nucleotides and proteins can be labeled by phosphorylation reactions. Nucleoti
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. Counting Cytostar-T scintillating microplates on the MicroBeta JET
3. Counting FlashPlates on the Wallac MicroBeta
4. Direct Counting of Millipore MultiScreen Filtration Plates.
5. Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter
6. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting
7. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
8. Comparing Fidelity and Performance of Proofreading PCR Enzymes
9. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
10. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
11. Performance Comparisons of Commercial RT-PCR Systems
Post Your Comments:
(Date:7/10/2014)... According to the International Atomic Energy Agency (IAEA) ... terrorists acquiring sufficient quantities of plutonium or highly ... explosive device. The IAEA also notes that most ... quantities, which can be challenging to detect with ... study appearing this week in the Journal ...
(Date:7/10/2014)... today in the journal PNAS (Proceedings of the ... of day and sleep deprivation have a significant effect ... looking at the best time of day to test ... for administering medicines effectively. , Researchers from the University ... investigated the links between sleep deprivation, body clock disruption ...
(Date:7/10/2014)... Wash. The lithium-ion batteries that power our laptops ... longer on a single charge with the help of ... porous material to replace the graphite traditionally used in ... than 10 times the energy storage capacity of graphite. ... battery electrode was published today in Nature Communications ...
(Date:7/10/2014)... recent study by researchers at the University of ... physical mechanisms governing the interplay of spin and ... limits of ultrafast spintronic devices for data storage ... charge as well as spin-angular momentum. In a ... there is no spin current," explained David Cahill, ...
Breaking Biology Technology:Sensitive detection method may help impede illicit nuclear trafficking 2Time of day crucial to accurately test for diseases, new research finds 2Silicon sponge improves lithium-ion battery performance 2Silicon sponge improves lithium-ion battery performance 3University of Illinois study advances limits for ultrafast nano-devices 2
... ... stick that can be shipped from stock greatly minimizing lead times for Semiconductor tool ... (PRWEB) ... specializing in process controls for the delivery of critical fluids, introduces an innovation to ...
... , PLEASANTON, Calif., FRAMINGHAM, Mass. ... THOR ), a world leader in device-based mechanical circulatory support ... International, Inc. (Nasdaq: HTWR ; ASX: HIN), which ... the United States Federal Trade Commission ("FTC") has informed them ...
... , , BETHESDA, Md., July ... company developing novel, proprietary antibodies for the treatment of cancer, inflammation ... of its common stock in a public offering. Piper Jaffray & ... Capital Markets as co-manager in this offering. The offering is ...
Cached Biology Technology:Aztech Controls Introduces Standardized Gas Sticks for Semiconductor Tool Hook Up 2Aztech Controls Introduces Standardized Gas Sticks for Semiconductor Tool Hook Up 3U.S. Federal Trade Commission to Challenge Thoratec's Proposed Acquisition of HeartWare International 2U.S. Federal Trade Commission to Challenge Thoratec's Proposed Acquisition of HeartWare International 3U.S. Federal Trade Commission to Challenge Thoratec's Proposed Acquisition of HeartWare International 4Micromet Announces Public Offering of Common Stock 2Micromet Announces Public Offering of Common Stock 3Micromet Announces Public Offering of Common Stock 4
(Date:7/11/2014)... July 11, 2014 Researchers have pioneered a ... The new technology, called Virtual Finger, allows scientists ... like neurons and synapses using the flat surface ... makes 3D imaging studies orders of magnitude more ... unprecedented level across many areas of experimental biology. ...
(Date:7/11/2014)... hippocampus, the marginal division of the striatum is ... the impact degree of substance P in the ... Yan Yu and his team, College of Biophotonics, ... staining, that substance P receptor, neurokinin 1 was ... division of normal rats. Unilateral or bilateral injection ...
(Date:7/11/2014)... , July 11, 2014 Research and ... "International Biometrics Technology Market - Industry Analysis Size Share ... their offering. http://photos.prnewswire.com/prnh/20130307/600769 ... crimes has created a need for high level security ... for personnel authentication such as username and passwords, tokens ...
Breaking Biology News(10 mins):Virtual finger enables scientists to navigate and analyze complex 3D images 2International Biometrics Technology Market - Industry Analysis Size Share Growth Trends and Forecast to 2019 2
... Prof. Makoto Tominaga and Dr. Takaaki Sokabe (National Institute ... in skin keratinocytes is important for formation and maintenance ... reported in the Journal of Biological Chemistry. ... namely "thermoTRPs". It is expressed in skin, acting as ...
... -- Astronauts are known to have a higher risk of ... go with weightlessness, confined crew quarters, being away from family ... while not getting enough sleep - are known to wreak ... by immunobiologist Ty Lebsack at the University of Arizona has ...
... same digital technology that enables young people to play video ... a simulated coral reef environment where they participate in science ... Field Museum scientists will link students from Chicago,s Austin neighborhood ... the Pacific Ocean, engage in the scientific process, and participate ...
Cached Biology News:Immune system compromised during spaceflight, study finds 2Immune system compromised during spaceflight, study finds 3Field Museum technology project wins prestigious award 2
Cytidine 5'-Triphosphate, Sodium (CTP), 1 g. Category: Nucleotides & Enzymes & Biochemicals, Nucleotides, Additional Nucleotide Products....
biotin-4-fluorescein...
... Consists of the LVF-HL, LVF-CUVADPT, FHS-LVF, and ... high-pass and low-pass Linear Variable Filters to ... an adjustable center wavelength and adjustable bandpass. ... (~90%) and blocking band (99.8%). These ...
BBS/Glycerol/Casein Block and Diluent - 10X Concentrate, 1 L...
Biology Products: