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Cenix-Designed siRNAs for 95% of Human and Mouse Genomes


siRNA designs now available for >95% of all human and mouse genes listed in RefSeq

Design algorithm developed by Cenix results in highly effective siRNAs

High quality custom siRNA synthesis from The RNA Company

Flexible options -- order individual, pre-designed siRNAs with your choice of synthesis options


Premium Quality siRNA Synthesis
Ambion's custom siRNA service provides the premium quality siRNAs you need for your research. Each siRNA is synthesized in our state-of-the-art chemical synthesis facility to meet the highest quality standards. Our standard purification procedure includes deprotection and column purification, and yields siRNA that is typically 90% pure (guaranteed >80% pure). For the most demanding applications, HPLC and PAGE purification is available, yielding siRNA that is guaranteed >97% pure.

To ensure that we are providing the highest quality siRNA, all HPLC and PAGE purified RNA oligonucleotides are analyzed for purity by capillary electrophoresis (CE) or HPLC. Furthermore, MALDI-TOF (matrix-assisted laser desorption ionization time-of-flight) mass spectrometry is used to analyze the molecular weight of each oligonucleotide (Figure 1). Finally, each annealed siRNA is analyzed by gel or capillary electrophoresis to ensure that the strands annealed properly.

Figure 1. MALDI-TOF Mass Spectrometry Analysis of an HPLC Purified Custom siRNA.


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Related biology technology :

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4. IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!
5. Optimizing Chemical Transfection and Electroporation of siRNAs
6. Delivering siRNAs to Difficult Cell Types
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