Navigation Links
Cells-to-cDNA II Applications

Quantitation of siRNA Target Gene Expression


RT-PCR is one of the easiest ways to confirm gene profiling and gene function analyses generated by methods such as array analysis and siRNA experiments, especially when there are many samples to analyze. However, there is one catch. For RT-PCR experiments, total RNA must be isolated first. Why not skip this step? Ambion scientists now demonstrate the use of the Cells-to-cDNA II Kit to produce real time RT-PCR data quickly, with minimal effort. Cells-to-cDNA cell lysates can be used for RT-PCR directly without RNA isolation. In this article, we show how the Cells-to-cDNA II Kit can be used to assess siRNA experiments for the development of an optimized RNA interference protocol. In addition to analyzing siRNA data, Cells-to-cDNA can be used to process FACS and LCM samples. The kit has also been adapted to 96 well plate automated processing. See "Automated Cells-to-cDNA Protocol" for the procedure.


Experimental Set-up: siRNA Transfection
HeLa cells were transfected with siRNA and an automated protocol for the Cells-to-cDNA II Kit was used to look at specificity of the siRNA effect and optimal cell concentration.

HeLa cells were examined under three experimental conditions: 1) cells that were transfected with a GAPDH siRNA, 2) cells that were transfected with a negative control siRNA and 3) untransfected cells. To optimize cell plating density fo
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Cells-to-cDNA II Using FACS Sorted Cells
2. Your Data: Detection of Oocyte-specific Gene Expression Using Ambions Cells-to-cDNA Kit
3. A Versatile Power Supply for All Electrophoresis Applications
4. Highest Possible Transformation Efficiencies for High-Throughput Applications
5. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
6. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
7. Optimizing Büchi® Rotary Evaporator Applications
8. Optimizing Büchi® Rotary Evaporator Applications
9. Optimizing Bchi Rotary Evaporator Applications
10. Uses and Applications of X-tremeGENE Q2 Transfection Reagent
11. Uses and Applications of FuGENE 6 Transfection Reagent

Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Cells cDNA Applications

(Date:11/5/2009)... LASIK Surgery and vision co...ons directory were actively involved at the recent...O) 2009 Annual Meeting in San Francisco, Californi...r, MD, Dr. Jonathan Davidorf, MD, Dr. Peter Rapoza... MD, Dr. R. Wayne Bowman, MD, Dr. Edward Manche, M...
(Date:11/4/2009)... This webinar, which will be...yone working in continuous flow chemistry processi...flow cell inline using various meso and micro scal...of over 670 publications has been recognized with ...ost recently the 2009 Tetrahedron Prize. , ...
(Date:11/4/2009)..., HUNTSVILLE, Ala., Nov. 4 DIATHE...X.com ), today presented at the Michigan Security...rence in Dearborn, Mich. Dennis Grimaud, Chairman ...d the impact of the company,s advanced molecular d...sponse, infectious disease diagnosis and military ...
(Date:11/4/2009)..., ,, ROCKVILLE, Md., Nov. 4 /PRNewswire/ -... company focused on next-generation antibody drug ...10 million in Series A funding from New Enterprise...ure capital firms. The capital will be used to cre...that address two or more biological targets with a...
Breaking Biology Technology:Leading Vision Correction Experts in Trusted LASIK Surgeons Directory Educate Fellow Doctors at AAO Annual Meeting 2Leading Vision Correction Experts in Trusted LASIK Surgeons Directory Educate Fellow Doctors at AAO Annual Meeting 3Leading Vision Correction Experts in Trusted LASIK Surgeons Directory Educate Fellow Doctors at AAO Annual Meeting 4Leading Vision Correction Experts in Trusted LASIK Surgeons Directory Educate Fellow Doctors at AAO Annual Meeting 5Leading Vision Correction Experts in Trusted LASIK Surgeons Directory Educate Fellow Doctors at AAO Annual Meeting 6Leading Vision Correction Experts in Trusted LASIK Surgeons Directory Educate Fellow Doctors at AAO Annual Meeting 7Leading Vision Correction Experts in Trusted LASIK Surgeons Directory Educate Fellow Doctors at AAO Annual Meeting 8Leading Vision Correction Experts in Trusted LASIK Surgeons Directory Educate Fellow Doctors at AAO Annual Meeting 9Professor Steven Ley of University of Cambridge will Present Free Webinar, 'Application of the ReactIR Flow Cell to Continuous Processing Technology' 2Professor Steven Ley of University of Cambridge will Present Free Webinar, 'Application of the ReactIR Flow Cell to Continuous Processing Technology' 3DIATHERIX Laboratories Presents at Homeland Security Conference 2Zyngenia, Inc. Raises $10 Million in Series A Funding 2Zyngenia, Inc. Raises $10 Million in Series A Funding 3Zyngenia, Inc. Raises $10 Million in Series A Funding 4
...pril 24 iGenix, Inc, today launched QTrait, a,per...beginning as low as,$99 per DNA test. iGenix co-fo...oped QTrait to create affordable DNA testing for t...privacy procedures in the genetics,industry. QTrai... leading,consumer privacy group that helps consume...
...of Cost Savings Initiatives Quantified - - ...e -, HOPKINTON, Mass., April 24 Caliper Life,Sci...f tools and services for,drug discovery and life s...d a plan to consolidate its west coast business op...ove productivity and effectiveness of,its research...
...N.J., April 24 Amicus Therapeutics,Inc. (Nasdaq: ...sident and,Chief Executive Officer, will present a...lthcare Unplugged Conference on Wednesday, April 3...ey Biscayne, Florida., The presentation will be w..., website at http://www.amicustherapeutics.com . ...
Other Biology Technology:Genetic Testing for All: QTrait Offers Industry's Least Expensive, Most Secure DNA Tests With TRUSTe Certification 2Genetic Testing for All: QTrait Offers Industry's Least Expensive, Most Secure DNA Tests With TRUSTe Certification 3Genetic Testing for All: QTrait Offers Industry's Least Expensive, Most Secure DNA Tests With TRUSTe Certification 4Caliper Life Sciences Reports West Coast Consolidation of Operations 2Caliper Life Sciences Reports West Coast Consolidation of Operations 3Caliper Life Sciences Reports West Coast Consolidation of Operations 4
(Date:11/4/2009)...PRNewswire-FirstCall/ -- BIO-key International, In...based biometric identification software is showcas...BB TXPO conference being held at the New Orleans C...will provide hands-on product demonstrations of it...oth #424. Also, at 5 PM on Monday, October 26, Pet...
(Date:11/3/2009)... 2009 -- The world,s greatest wines are the resul...n input, and something called the "terroir" -- a t...climate. , Experts will discuss terroir with a...sday, Nov. 4 in Pittsburgh. "Terroir: Winegrapes ...s at the 2009 Annual Meetings of the American Soci...
(Date:11/3/2009)...e women are as much as 28 percent less likely to b...cording to research that earned a Michigan State U...ings by Barbara Luke, a researcher in the MSU Coll...Gynecology and Reproductive Biology, focused on da...ive technology. , Luke,s findings, which also ca...
Breaking Biology News(10 mins):BIO-key(R) International Exhibiting at AABB TXPO 2Great wines come from great soils 2MSU researcher: Obesity significantly cuts odds of successful pregnancy 2MasterControl and Datafarm Launch GxP2eCTD at the DIA 45th Annual Meeting 12748 1MasterControl and Datafarm Launch GxP2eCTD at the DIA 45th Annual Meeting 12748 2MasterControl and Datafarm Launch GxP2eCTD at the DIA 45th Annual Meeting 12748 3MasterControl and Datafarm Launch GxP2eCTD at the DIA 45th Annual Meeting 12748 4ASAP Systems Launches New Depreciation Feature Enabling its Customers to Develop Custom Formulas to Calculate Depreciation Of Their Fixed Assets Inven 49762 1ASAP Systems Launches New Depreciation Feature Enabling its Customers to Develop Custom Formulas to Calculate Depreciation Of Their Fixed Assets Inven 49762 2Dr Susan Blumenthal Meridian International Centers Global Health Chair Named Health Leader of the Year 49759 1Dr Susan Blumenthal Meridian International Centers Global Health Chair Named Health Leader of the Year 49759 2Dr Susan Blumenthal Meridian International Centers Global Health Chair Named Health Leader of the Year 49759 3
...d the occasion of the dedication of the Robert J. ...niversitys 1604 Campus to announce an $822,000 gif...wo Texans. , For some time, we have wanted to cre...ilanthropy, said Romo. So, several months ago, we...exas System to approve the naming of one of our mo...
...atement was,issued today by the U.S. Department of...n. I want to thank Interior Minister Schauble and ...ing our visit and for their,dedication to maintain...es,and Germany., The cooperation between our two ...d counterterrorism has been substantial, and citiz...
...nt she?) respond to the mating call of her suitors...souri, one species of katydid may owe its ecologic...ty of male katydids to adjust their mating calls t... Neoconocephalus triops, which can be found from P...for mating that change with the seasons. The males...
Other Biology News:UTSA receives $822,000 from Kleberg Foundation to enhance scientific research 2Remarks Prepared for Delivery By Attorney General Michael B. Mukasey At the Ministerial Press Conference 2Female katydids prefer mates 'cool' in winter and 'hot' in summer 2
ElectroZap from Ambion
...ders combines reagent dispensing with fluorescence...dable, easy-to-use platform for the complete range...design and adaptability of the Infinite 200 series...ator (M200) or filter (F200) system and allows you...
...ality, Functionally-Validated, Ion Channel Cell Li...itical role in nerve and muscle function and conse...eart. Drugs that modulate ion channels have been i...hic pain, cardiac arrhythmia, hypertension, local ...
Mouse Anti-Human ARHGEF11 Monoclonal Antibody, Unconjugated, Clone 2F4 from Abnova Corporation
Biology Products: