Navigation Links
Cell lines expressing recombinant aequorin and a G-protein coupled receptor for functional screening

using the MicroLumat single-detector, singleinjector luminometer.

CHO-CCR5-Gα16-apoaequorin cells were injected onto increasing (from column 1 to 12) concentrations of RANTES (rows A and B, same dilutions in duplicate), MIP1α (rows C and D, duplicates), MIP1β (rows E and F, duplicates), and another analog of RANTES (rows G and H, duplicates). The emitted light was recorded for 30 seconds and the intensity of the emitted light was plotted as a function of time. The scaling is the same for all the graphs presented here.

Agonist-induced light emission in CHO cells expressing the CCR5 receptor

Human chemokine CCR3 receptor:

A K562 cell line expressing the chemokine CCR3 receptor, the Gα16 coupling protein and apoaequorin was established. Dose-response curves were obtained as described above and results are shown in figure 4.

Human chemokine CCR8 receptor:

CHO cells expressing the chemokine CCR8 receptor were transfected with a plasmid encoding the Gα16 coupling protein and apoaequorin and stable transfectants were selected with antibiotic. The mix of clones obtained from this transfection was used to generate a dose-response curve with I-309 as described above. Results are shown in figure 5.

Human serotonin 5HT2B receptor:

A CHO cell line expressing the serotonin 5HT2B receptor, the Gα16 coupling protein and apoaequorin was established.

A. Detection of agonists

Dose-response curves for agonists of the 5HT2B receptor were obtained as described above and results are shown in figure 6A.

B. Dectection of antagonists

For t
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. siRNA Design Guidelines
2. Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3, 5 monophosphate and its validation for pharmacological screening at G protein-coupled receptors
3. Gq-coupled Receptor Assays Using FLEXstation
4. Gq-coupled receptor assays using FLEXstation: M1 muscarinic receptor
5. Gq-coupled receptor assays: a comparison of FLEXstation and FLIPR
6. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
7. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
8. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
9. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
10. Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000
11. The use of CypHer5 for receptor internalization studies in both a range of GPCRs and a non-GPCR
Post Your Comments:
(Date:9/8/2008)...ieve undetectable HCV RNA levels, following 4 week...R) plus Copegus(R) -,...dministered with Pegasys ...wswire-FirstCall/ -- Pharmasset, Inc.,(Nasdaq: VR...th cohort of a,4-week Phase 1 proof-of-concept cli...
(Date:9/8/2008)...ement Consulting announced that it has appointed A... Charlotte, NC (PRWEB) September 8, 2008...ment Consulting] announced that it has appointed A...len joins Symphony from Wellesley Information Serv... developing, and producing national educational co...
(Date:9/6/2008)...elps visitors to electronica 2008 in Munich and co...ctronica information available on the visitor hotl...nning a trip, online registration benefits, a summ...ludes an extensive list of web links to travel, ho... Munich, Germany (PRWEB) September 6, 2008 -...
(Date:9/5/2008)...stitute of Standards and Technology (NIST) and Seo...weak a new class of polymer-based semiconductors t... components of the blend. Their recent resultshow ...gn of practical, large-scale manufacturing techniq...onic displays and other devices.* , Organic semi...
Breaking Biology Technology:Pharmasset Reports Preliminary Results of a 4-week Proof-of-Concept Combination Study of R7128 for the Treatment of Chronic Hepatitis C in Genotype 2 or 3 Non-Responders 2Pharmasset Reports Preliminary Results of a 4-week Proof-of-Concept Combination Study of R7128 for the Treatment of Chronic Hepatitis C in Genotype 2 or 3 Non-Responders 3Pharmasset Reports Preliminary Results of a 4-week Proof-of-Concept Combination Study of R7128 for the Treatment of Chronic Hepatitis C in Genotype 2 or 3 Non-Responders 4Pharmasset Reports Preliminary Results of a 4-week Proof-of-Concept Combination Study of R7128 for the Treatment of Chronic Hepatitis C in Genotype 2 or 3 Non-Responders 5Pharmasset Reports Preliminary Results of a 4-week Proof-of-Concept Combination Study of R7128 for the Treatment of Chronic Hepatitis C in Genotype 2 or 3 Non-Responders 6Symphony Managemnt Consulting Hires New Solutions Director 2Free White Paper Now Available: How to get the most from your visit to electronica 2008 in Munich 2Free White Paper Now Available: How to get the most from your visit to electronica 2008 in Munich 3Bottoms up: Better organic semiconductors for printable electronics 2
... chalk outlines of a body, but companies need to p...emedies, said a lawyer who spoke at the Fusion 20...aro, a partner with Michael Best & Friedrich , sa...romised by Internet fraudsters or disgruntled empl...nforcement. , ,"You get to ride on the backs of ex...
...essionals might know technology, but business exec...expert panel at the Fusion 2005 CEO-CIO Symposium...speak for executives and examined ways a chief inf... ,Good IT converts data into dollars and knowledge...director of the Mason Wells investment firm. , ,...
...talled its first volume computed tomography scanne...used exclusively by a cardiologist. , ,Round Rock ...o quickly scan patients, hearts and nearby arterie...tbeats. By rotating and quickly creating 64 images... ,The scanner entered general clinical use at Fro...
...he Madison area has developed a research-based eco...on has been a massive, unified engine for technolo... have generally been smart enough to capitalize on...n,t so fortunate as to have one of the nation,s la...has UW-Milwaukee, the Medical College of Wisconsin...
Other Biology Technology:After a successful attack on your computer systems, then what? 2After a successful attack on your computer systems, then what? 3CIOs learn boardroom-speak 2CIOs learn boardroom-speak 3Milwaukee could boom with explosion of information technology in medical care 2Milwaukee could boom with explosion of information technology in medical care 3
(Date:9/7/2008)...lated plants produce their own natural chemical co...al plant,s specific habitat. Comparing anti-fungal...the Salk Institute for Biological Studies discover...nough to shift the whole output to an entirely new...xture of henbane and tobacco-specific molecules an...
(Date:9/5/2008)... Society is pleased to announce the recipients of ... receive their awards at the Society,s 53rd Annual...nvention and Exhibitions Center in Massachusetts. ...sity of California, San Francisco, will receive th...ing contributions to transmembrane biology, and pi...
(Date:9/5/2008)...g a sophisticated computer model pioneered at Prin...found that many governments worldwide are recommen...ite the availability of many drugs and therapies t... recommend using what is known as a single first-l...with many patients. Writing in the Sept. 16 issue ...
(Date:9/5/2008)...vative work on a new type of heart stent sensor is...f mechanical engineering at Virginia Tech, a Natio...pment (CAREER) award of $400,000. , The focus o...ific models and experiments to describe what happe...s a unique type of in situ polymer strain-sensing ...
Breaking Biology News(10 mins):How plants fine tune their natural chemical defenses 2How plants fine tune their natural chemical defenses 3Biophysical Society names 2009 award recipients 2Best way to treat malaria: Avoid using same drug for everyone, scientists say 2Best way to treat malaria: Avoid using same drug for everyone, scientists say 3Goulbourne earns NSF award to research heart stent sensors 2Goulbourne earns NSF award to research heart stent sensors 3Best code for disease detection bar none 3677 1New discoveries from Harvard and Baylor get to the heart of cardiovascular disease 3675 1New discoveries from Harvard and Baylor get to the heart of cardiovascular disease 3675 2New discoveries from Harvard and Baylor get to the heart of cardiovascular disease 3675 3Advance towards early Alzheimers diagnosis 22166 1Advance towards early Alzheimers diagnosis 22166 2Premier Brumby announces plans to build largest life sciences supercomputer facility 22164 1Premier Brumby announces plans to build largest life sciences supercomputer facility 22164 2Premier Brumby announces plans to build largest life sciences supercomputer facility 22164 3
... the species known as social amoebas has been plot...provide important clues to the evolution of life o...logist Professor Sandie Baldauf, of the University... of the University of Dundee, have produced the fi...species of social amoeba. , Using this family t...
... haphazardly flail about looking for a host but se...d identify potential hosts by their emissions, acc.... , "We are interested in how plants respond to th...nteractions," says Dr. Consuelo M. De Moraes, assi... to see how little was available on how above-grou...
...lebee will carry its pollen next, you have to lite...raging style that,s been successful, they are more...n learning something new," says Rebecca Flanagan, ...Milwaukee (UWM). , Flanigan and her professor, J...as they gather pollen ?which plant species the bee...
...ntified a key enzyme responsible for triggering a ... according to new study findings published online ...archers from Virginia Commonwealth University, the...dical College in New York sets the stage for devel... control allergic disease ?the sixth leading cause...
Other Biology News:Scientists' cell discovery unearths evolutionary clues 2Scientists' cell discovery unearths evolutionary clues 3Parasitic plants sniff out hosts 2Parasitic plants sniff out hosts 3Researchers find that bumblebees' flower choice matters 2Researchers find that bumblebees' flower choice matters 3Researchers find that bumblebees' flower choice matters 4Key molecular signaling switch involved in allergic disease identified 2
...it Polyclonal to Human CELSR3 CELSR3 is an Orpha...ene is expressed during development at sites of ac...ain, spinal cord, dorsal root ganglion, and eye. E...is, blood, brain, colon, heart/melanocyte/uterus, ...
...ination inline heater plus multi-channel focal dru...ug wash-out with a high-flow bath line while quick...e Perfusion Pencil.® Steady 37°C at 5 ml/...ip. Advanced auto-tuning temperature lock Desig...
Immunogen: purified recombinant human angiogenin expressed in E. coli . Physical form: Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline. Titertest method: ELISA (c).
CKB Antibody (N-term) Antigen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected within aa 1~100 of human CKB. Molecular Weight: 42644 Da
Biology Products: