Navigation Links
Cell lines expressing recombinant aequorin and a G-protein coupled receptor for functional screening

using the MicroLumat single-detector, singleinjector luminometer.

CHO-CCR5-Gα16-apoaequorin cells were injected onto increasing (from column 1 to 12) concentrations of RANTES (rows A and B, same dilutions in duplicate), MIP1α (rows C and D, duplicates), MIP1β (rows E and F, duplicates), and another analog of RANTES (rows G and H, duplicates). The emitted light was recorded for 30 seconds and the intensity of the emitted light was plotted as a function of time. The scaling is the same for all the graphs presented here.

Agonist-induced light emission in CHO cells expressing the CCR5 receptor

Human chemokine CCR3 receptor:

A K562 cell line expressing the chemokine CCR3 receptor, the Gα16 coupling protein and apoaequorin was established. Dose-response curves were obtained as described above and results are shown in figure 4.

Human chemokine CCR8 receptor:

CHO cells expressing the chemokine CCR8 receptor were transfected with a plasmid encoding the Gα16 coupling protein and apoaequorin and stable transfectants were selected with antibiotic. The mix of clones obtained from this transfection was used to generate a dose-response curve with I-309 as described above. Results are shown in figure 5.

Human serotonin 5HT2B receptor:

A CHO cell line expressing the serotonin 5HT2B receptor, the Gα16 coupling protein and apoaequorin was established.

A. Detection of agonists

Dose-response curves for agonists of the 5HT2B receptor were obtained as described above and results are shown in figure 6A.

B. Dectection of antagonists

For t
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. siRNA Design Guidelines
2. Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3, 5 monophosphate and its validation for pharmacological screening at G protein-coupled receptors
3. Gq-coupled Receptor Assays Using FLEXstation
4. Gq-coupled receptor assays using FLEXstation: M1 muscarinic receptor
5. Gq-coupled receptor assays: a comparison of FLEXstation and FLIPR
6. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
7. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
8. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
9. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
10. Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000
11. The use of CypHer5 for receptor internalization studies in both a range of GPCRs and a non-GPCR
Post Your Comments:
(Date:9/18/2014)... (PRWEB) September 18, 2014 Leatherwood ... FM-4910 listed material, to stay competitive in the ... These high-performance materials ultimately impact product quality and ... and owner. , Established in 1980 in Lewisville, ... as many fabricators have left the business. Much ...
(Date:9/18/2014)... , Sept. 18, 2014 /PRNewswire/ - Oncothyreon Inc. ... of previously announced concurrent but separate underwritten offerings of ... to the public of $2.00 per share, for expected ... its Series A Convertible Preferred Stock at a price ... gross proceeds of $20 million. Each share of Series ...
(Date:9/18/2014)... September 18, 2014 SeqLL Inc., ... (tSMS™) technology, announced today the closing of a ... led by Genomic Diagnostic Technologies, will increase the ... applications. , Founded in March 2013 by Daniel ... Analyst at Helicos BioSciences, SeqLL has continued to ...
(Date:9/18/2014)... developed a new microscopy technology that allows them ... higher-than-ever resolution. , Dubbed "Complementation Activated Light Microscopy" ... are an order of magnitude finer than conventional ... of biomolecules at the nanometer scale. , In ... Nature Communications , the researchers behind CALM used ...
Breaking Biology Technology:Leatherwood Plastics Selects Vycom’s Flametec PVC-C Material to Stay Competitive in Semiconductor and Lab Equipment Industries 2Oncothyreon Prices $40 Million Concurrent Public Offerings of Common Stock and Preferred Stock 2Oncothyreon Prices $40 Million Concurrent Public Offerings of Common Stock and Preferred Stock 3SeqLL Closes Series-A Funding Round for Expansion of Single Molecule Sequencing 2Scientists pioneer microscopy technique that yields fresh data on muscular dystrophy 2
... of revenue growth ... 2007 Highlights: * Product ... SET pulse oximeter units increase 20% to 116,300 units, IRVINE, Calif., Feb. ... Measure-Through,Motion & Low Perfusion pulse oximetry, today announced its financial,results for both the ...
... Replidyne, Inc.,(Nasdaq: RDYN ) today announced its financial results ... Replidyne reported a net loss of $16.9 million for ... per basic and diluted common share,of $0.63 per share, compared ... and diluted common share, for the quarter ended December 31, ...
... Market Research,a global provider of pharmaceutical competitive intelligence ... critical strategic insight,for pharma and biotech companies with ... therapies., In this report, entitled MedPredict Thought ... global Thought Leader Panel,debates tough questions facing drug ...
Cached Biology Technology:Masimo Reports Fourth Quarter 2007 and Full Year 2007 Financial Results 2Masimo Reports Fourth Quarter 2007 and Full Year 2007 Financial Results 3Masimo Reports Fourth Quarter 2007 and Full Year 2007 Financial Results 4Masimo Reports Fourth Quarter 2007 and Full Year 2007 Financial Results 5Masimo Reports Fourth Quarter 2007 and Full Year 2007 Financial Results 6Masimo Reports Fourth Quarter 2007 and Full Year 2007 Financial Results 7Masimo Reports Fourth Quarter 2007 and Full Year 2007 Financial Results 8Masimo Reports Fourth Quarter 2007 and Full Year 2007 Financial Results 9Masimo Reports Fourth Quarter 2007 and Full Year 2007 Financial Results 10Masimo Reports Fourth Quarter 2007 and Full Year 2007 Financial Results 11Masimo Reports Fourth Quarter 2007 and Full Year 2007 Financial Results 12Replidyne Announces 2007 Fourth Quarter and Full Year Results 2Replidyne Announces 2007 Fourth Quarter and Full Year Results 3Replidyne Announces 2007 Fourth Quarter and Full Year Results 4Replidyne Announces 2007 Fourth Quarter and Full Year Results 5Replidyne Announces 2007 Fourth Quarter and Full Year Results 6Replidyne Announces 2007 Fourth Quarter and Full Year Results 7Unmet Needs Provide Challenges and Opportunities for Rheumatoid Arthritis Drug Developers According to MedPredict Report 2
(Date:9/18/2014)... icy moon of Uranus, is one of the most ... Despite its relatively small size, Miranda appears to have ... the formation of at least three remarkable and unique ... These coronae are visible in Miranda,s southern hemisphere, and ... corona, the largest, has ridges and troughs with up ...
(Date:9/18/2014)... University and the Quebec government have discovered microplastics ... Journal of Fisheries and Aquatic Sciences . , ... or industrial cleansers, to which they are commonly ... and buoyancy, they may readily pass through sewage ... in the world,s oceans, but have only recently ...
(Date:9/18/2014)... The fall foliage season that prompts millions of Americans to ... later and possibly last a little longer within a century, ... leaf peeping in some areas of the United States as ... report in the journal Global Ecology and Biogeography . ... that is the state tree of New Hampshire could ...
Breaking Biology News(10 mins):Miranda: An icy moon deformed by tidal heating 2Miranda: An icy moon deformed by tidal heating 3Miranda: An icy moon deformed by tidal heating 4Miranda: An icy moon deformed by tidal heating 5Miranda: An icy moon deformed by tidal heating 6Miranda: An icy moon deformed by tidal heating 7Microplastic pollution discovered in St. Lawrence River sediments 2Fall foliage season may be later, but longer on warmer Earth 2Fall foliage season may be later, but longer on warmer Earth 3Fall foliage season may be later, but longer on warmer Earth 4
... the laboratory of Assistant Professor Frank J. Slack at Yale ... is regulated in lung cancer, presenting new possibilities for diagnosis ... the journals Cell and Developmental Cell. , The oncogene Ras ... where it is over-expressed or activated by mutation. According to ...
... prevented allergic reactions in laboratory mice, as well as ... California, Los Angeles (UCLA) researchers report in the April ... future, the investigators say, these promising results could lead ... allergies, but also possibly for severe food allergies such ...
... dead bone of a transplanted skeletal graft into living tissue ... therapy to stimulate the body into treating the foreign splint ... of cancer and trauma patients each year who suffer with ... Feb. 13 and will appear in the March 1 issue ...
Cached Biology News:Yale Scientists Find MicroRNA Regulates Ras Cancer Gene 2Novel Therapy Tested in Mice Could Chase Away Cat Allergies 2Novel Therapy Tested in Mice Could Chase Away Cat Allergies 3Gene therapy converts dead bone graft to new, living tissue 2Gene therapy converts dead bone graft to new, living tissue 3Gene therapy converts dead bone graft to new, living tissue 4
Mouse monoclonal antibody raised against a partial recombinant PRKG1. NCBI Entrez Gene ID = PRKG1...
... polyclonal antibody raised against a ... Immunogen: PPP4C (NP_002711, ... partial recombinant protein with GST ... Number: NM_002720 ...
Mouse monoclonal antibody raised against a full length recombinant GPT. NCBI Entrez Gene ID = GPT...
Mouse monoclonal antibody raised against a partial recombinant CAPG. NCBI Entrez Gene ID = CAPG...
Biology Products: