( WST-1 was addedand the cells were ...)Cell Proliferation and Viability Measurement

WST-1 was added, and the cells were reincubated for another 2 hours. Then, the formazan product was quantified at 450 nm with an absorbance reader (Figure 3). Subsequently, BrdU incorporation was determined using the Cell Proliferation ELISA, BrdU (colorimetric). The results show that Actinomycin D inhibits DNA synthesis, but it does not inhibit the metabolic activity of the cell. Thus, actinomycin D is cytostatic (inhibits DNA synthesis) but not cytotoxic (no inhibition of metabolic activity).

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