( ameters: metabolic activity or cell mem...)Cell Proliferation and Viability Measurement

ameters: metabolic activity or cell membrane integrity of healthy cells. Usually the metabolic activity is measured in cell populations via incubation with a tetrazolium salt (e.g., MTT, XTT, WST-1) that is cleaved into a colored formazan product by metabolically active cells.

The ATP status of cells can also be analyzed and gives an indication for cellular energy capacity and thus viability.
The second type of assay also termed dye-exclusion assay takes advantage of the ability of healthy cells with uncompromised cytoplasmic membrane integrity to exclude dyes such as trypan blue. Dead cells will be stained, and healthy cells can be counted directly. In cytotoxicity studies, this ability is also analyzed; however, in these assays, molecules are released from dying cells with leaky cytoplasmic membranes, and are measured in cell populations (e.g., lactate dehydrogenase or [51Cr] release).

Assay Procedures for Cell Proliferation and Viability Kits

Roche Applied Science offers a broad range of reliable and easy-to-use products for the analysis of cell proliferation and viability. Selected examples are given in Figures 1 and 2. The assay procedures for microplate tests, which are particularly useful for routine applications in high-volume labs, are summarized in Figure 1. Figure 2 shows the workflow for an application on individual cells.

Simulateous measurement of cell proliferation and cell viability

A549 cells (1 x 104/well in 100 ml) were incubated in the presence of varying amounts of actinomycin D for 20 hours. After labeling of the cells with BrdU for 2 hours, Cell Proliferation Reagent
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