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Cell Proliferation and Viability Measurement

The quantification of cellular growth, including proliferation and viability, has become an essential tool in any laboratory working on cell-based studies. Such techniques enable not only the optimization of cell culture conditions, but also the determination of growth factor and cytokine activity. Even more importantly, the efficacy of therapeutic agents in drug screening, the cytostatic potential of anticancer compounds in toxicology testing, and cell-mediated toxicity can be assessed when quantifying cell growth.
The first step in any experiment is the decision whether to test for cell proliferation or viability parameters, depending on the objective of a study. These parameters are measured by assaying for vital functions that are characteristic for healthy or growing cells.

But how are the terms cell proliferation and viability defined?

Cell viability measurements assess healthy cells in a sample. This can be accomplished either by directly counting the number of healthy cells or by measuring an indicator for healthy cells in cell populations (e.g., in a microplate assay). Whether the cells are actively dividing or quiescent is not distinguished. An increase in cell viability indicates cell growth, while a decrease in viability can be interpreted as the result of either toxic effects of compounds/agents or suboptimal culture conditions.
In contrast to cell viability analysis, cell proliferation assessment is defined as the measurement of actively dividing cells in a sample. It can be expressed either as the actual number or proportion of proliferating cells in cell culture, tissues, or as relative values in assays for cell populations. Quiescent nongrowing he
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Related biology technology :

1. Cell Proliferation Kit Outperforms the Competition in the Range of Cells Typically Counted
2. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
3. measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay
4. Cell Proliferation Assay Using the Benchmark Plus Microplate Spectrophotometer
5. Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000
6. Cell Proliferation Assays
7. LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)
8. Promegas Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar
9. Promegas Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar
10. LabVIEW for Measurement and Data Analysis
11. Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor
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