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Cell Proliferation Kit Outperforms the Competition in the Range of Cells,,,Typically Counted

and quantifying cell number. The number of attached cells plateau at 50,000 cells, regardless of how many additional cells are added; this indicates the approximate maximum number of monolayer cells that can attach on a 96-well format. The number of cells required for approximate 100% confluency in 48-well and 24-well formats were estimated using a relative well-floor surface area.

Therefore, suspension cell cultures can significantly exceed the 50,000 cells per well in 96-well culture dishes. These findings suggest that the number of cells per well can easily exceed 50,000 in many cell lines, even in 96-well culture dishes.

Conclusions

To quickly and accurately measure cells at numbers that can exceed 50,000 cells per well, the Quantos cell proliferation assay kit is superior to other commercially available products. The kit accurately quantitates cell number by providing a linear relationship of cell number versus fluorescence units, in the cell range typically found in common tissue culture well formats; thus, counting cell number is more accurate.


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