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Cell Proliferation Kit Outperforms the Competition in the Range of Cells,,,Typically Counted

culture dishes. Since CHO cells are adherent cells and typically form a monolayer, this test demonstrates the minimum number of cells that will cover the well floor in a 96-well culture dish. As Table 1 shows, even these cells will exceed 50,000 in cell culture dish formats larger than 96-well. Many other common cell lines, such as COS-1, form multiple layers of cells and grow to over 100,000 cells per well in 96-well culture dishes (data not shown). Additionally, suspension cell types typically grow to densities of 1,000,000 cells/ml, resulting in 200,000 cells per 200 l sample.

Table 1
Monolayer Adherent Cells at Approximate 100% Confluency

Number of Cells in Various Dish Formats

Format:

96 well

48 well

24 well

Cells:

50,000

100,000

350,000

The number of CHO cells required for 100% confluency in 96-well format culture dishes were determined by adding serial dilutions, allowing cells to attach over four hours, then rinsing
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